Aptamer-Directed Conjugation of DNA to Therapeutic Antibodies
A method for aptamer directed conjugation of DNA to therapeutic antibodies has been developed. In the method, an antibody selective aptamer binds to a specific site in the constant domain of human IgG1 antibodies and is used for both templated and direct conjugation to the antibodies. Through optimi...
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| Veröffentlicht in: | Bioconjugate chemistry 2019-08, Vol.30 (8), p.2127-2135 |
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| container_title | Bioconjugate chemistry |
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| creator | Skovsgaard, Mikkel B Mortensen, Michael R Palmfeldt, Johan Gothelf, Kurt V |
| description | A method for aptamer directed conjugation of DNA to therapeutic antibodies has been developed. In the method, an antibody selective aptamer binds to a specific site in the constant domain of human IgG1 antibodies and is used for both templated and direct conjugation to the antibodies. Through optimization of the design and reaction conditions, the antibody–DNA conjugates could be produced efficiently using equal stoichiometry of protein and DNA. Three different antibodies were evaluated, and the conjugates were characterized by anion exchange chromatography and SDS-PAGE. The conjugation sites for one of the antibodies were determined by MS/MS analysis of the digested conjugate. The antibody–DNA conjugate was also tested for receptor binding on cell surfaces showing retained binding. |
| doi_str_mv | 10.1021/acs.bioconjchem.9b00363 |
| format | Article |
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| ispartof | Bioconjugate chemistry, 2019-08, Vol.30 (8), p.2127-2135 |
| issn | 1043-1802 1520-4812 |
| language | eng |
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| source | ACS Journals: American Chemical Society Web Editions |
| subjects | Anion exchange Anion exchanging Antibodies Aptamers Binding Conjugates Conjugation Deoxyribonucleic acid Design optimization DNA Gel electrophoresis Immunoglobulin G Immunoglobulins Sodium lauryl sulfate Stoichiometry |
| title | Aptamer-Directed Conjugation of DNA to Therapeutic Antibodies |
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