Up-regulation of microRNA-136 induces apoptosis and radiosensitivity of esophageal squamous cell carcinoma cells by inhibiting the expression of MUC1
This present study is performed to figure out the role of microRNA-136 (miR-136) in radiosensitivity of esophageal squamous cell carcinoma (ESCC) cells through the regulation of MUC1. Seventy-four ESCC patients were divided into radiotherapy sensitive group and radiotherapy resistance group. Colony...
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| Veröffentlicht in: | Experimental and molecular pathology 2019-10, Vol.110, p.104278-104278, Article 104278 |
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| creator | Huang, Hua-Zhong Yin, Yi-Fa Wan, Wen-Jun Xia, Dan Wang, Rui Shen, Xue-Min |
| description | This present study is performed to figure out the role of microRNA-136 (miR-136) in radiosensitivity of esophageal squamous cell carcinoma (ESCC) cells through the regulation of MUC1.
Seventy-four ESCC patients were divided into radiotherapy sensitive group and radiotherapy resistance group. Colony formation assay and flow cytometry were used to test the radiosensitivity of radiotherapy resistant strain and parent strain. The expression of miR-136 between radiotherapy resistant strain and parent strain was detected by RT-qPCR, and the expression of miR-136 in Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells was detected after different doses of X-ray irradiation. Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells with overexpression of miR-136 or co-overexpression of miR-136 and MUC1 were constructed. Cell proliferation, colony formation and apoptosis was detected by CCK-8 assay, colony formation assay, and flow cytometry, respectively.
The expression of miR-136 in ESCC tissues was lower and MUC1 mRNA and protein expression was higher than that in adjacent normal tissues. The expression of miR-136 was negatively correlated with the expression of MUC1 mRNA in ESCC. Low expression of miR-136 and high expression of MUC1 were associated with tumor size, lymph node metastasis and distant metastasis. The expression of miR-136 increased while the expression of MUC1 decreased in the radiotherapy sensitive group of ESCC patients relative to the radiotherapy resistant group. The colony formation ability of radiation resistant cell line was stronger than that of parent cell line, and the apoptosis rate showed an opposite trend. Up-regulation of miR-136 reduced the survival rate, suppressed colony formation ability and induced apoptosis of ESCC cells under irradiation, which was reversed by upregulated MUC1.
This study demonstrates that up-regulation of miR-136 induces apoptosis and radiosensitivity of ESCC cells by inhibiting the expression of MUC1. |
| doi_str_mv | 10.1016/j.yexmp.2019.104278 |
| format | Article |
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Seventy-four ESCC patients were divided into radiotherapy sensitive group and radiotherapy resistance group. Colony formation assay and flow cytometry were used to test the radiosensitivity of radiotherapy resistant strain and parent strain. The expression of miR-136 between radiotherapy resistant strain and parent strain was detected by RT-qPCR, and the expression of miR-136 in Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells was detected after different doses of X-ray irradiation. Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells with overexpression of miR-136 or co-overexpression of miR-136 and MUC1 were constructed. Cell proliferation, colony formation and apoptosis was detected by CCK-8 assay, colony formation assay, and flow cytometry, respectively.
The expression of miR-136 in ESCC tissues was lower and MUC1 mRNA and protein expression was higher than that in adjacent normal tissues. The expression of miR-136 was negatively correlated with the expression of MUC1 mRNA in ESCC. Low expression of miR-136 and high expression of MUC1 were associated with tumor size, lymph node metastasis and distant metastasis. The expression of miR-136 increased while the expression of MUC1 decreased in the radiotherapy sensitive group of ESCC patients relative to the radiotherapy resistant group. The colony formation ability of radiation resistant cell line was stronger than that of parent cell line, and the apoptosis rate showed an opposite trend. Up-regulation of miR-136 reduced the survival rate, suppressed colony formation ability and induced apoptosis of ESCC cells under irradiation, which was reversed by upregulated MUC1.
This study demonstrates that up-regulation of miR-136 induces apoptosis and radiosensitivity of ESCC cells by inhibiting the expression of MUC1.</description><identifier>ISSN: 0014-4800</identifier><identifier>EISSN: 1096-0945</identifier><identifier>DOI: 10.1016/j.yexmp.2019.104278</identifier><identifier>PMID: 31247211</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Apoptosis ; Apoptosis - genetics ; Apoptosis - radiation effects ; Cell Line, Tumor ; Cell Proliferation - genetics ; Cell Proliferation - radiation effects ; Cell Survival - genetics ; Cell Survival - radiation effects ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - radiotherapy ; Esophageal squamous cell carcinoma ; Esophageal Squamous Cell Carcinoma - genetics ; Esophageal Squamous Cell Carcinoma - radiotherapy ; Female ; Gene Expression Regulation, Neoplastic - radiation effects ; Humans ; Male ; microRNA-136 ; MicroRNAs - genetics ; Middle Aged ; MUC1 ; Mucin-1 - genetics ; Radiation Tolerance - genetics ; Radiosensitivity ; Up-Regulation ; X-Rays</subject><ispartof>Experimental and molecular pathology, 2019-10, Vol.110, p.104278-104278, Article 104278</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-7137a96625800e63d5df11915e938791b98946b646ad8e29a164312f1cdf54183</citedby><cites>FETCH-LOGICAL-c359t-7137a96625800e63d5df11915e938791b98946b646ad8e29a164312f1cdf54183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexmp.2019.104278$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31247211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Hua-Zhong</creatorcontrib><creatorcontrib>Yin, Yi-Fa</creatorcontrib><creatorcontrib>Wan, Wen-Jun</creatorcontrib><creatorcontrib>Xia, Dan</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><creatorcontrib>Shen, Xue-Min</creatorcontrib><title>Up-regulation of microRNA-136 induces apoptosis and radiosensitivity of esophageal squamous cell carcinoma cells by inhibiting the expression of MUC1</title><title>Experimental and molecular pathology</title><addtitle>Exp Mol Pathol</addtitle><description>This present study is performed to figure out the role of microRNA-136 (miR-136) in radiosensitivity of esophageal squamous cell carcinoma (ESCC) cells through the regulation of MUC1.
Seventy-four ESCC patients were divided into radiotherapy sensitive group and radiotherapy resistance group. Colony formation assay and flow cytometry were used to test the radiosensitivity of radiotherapy resistant strain and parent strain. The expression of miR-136 between radiotherapy resistant strain and parent strain was detected by RT-qPCR, and the expression of miR-136 in Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells was detected after different doses of X-ray irradiation. Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells with overexpression of miR-136 or co-overexpression of miR-136 and MUC1 were constructed. Cell proliferation, colony formation and apoptosis was detected by CCK-8 assay, colony formation assay, and flow cytometry, respectively.
The expression of miR-136 in ESCC tissues was lower and MUC1 mRNA and protein expression was higher than that in adjacent normal tissues. The expression of miR-136 was negatively correlated with the expression of MUC1 mRNA in ESCC. Low expression of miR-136 and high expression of MUC1 were associated with tumor size, lymph node metastasis and distant metastasis. The expression of miR-136 increased while the expression of MUC1 decreased in the radiotherapy sensitive group of ESCC patients relative to the radiotherapy resistant group. The colony formation ability of radiation resistant cell line was stronger than that of parent cell line, and the apoptosis rate showed an opposite trend. Up-regulation of miR-136 reduced the survival rate, suppressed colony formation ability and induced apoptosis of ESCC cells under irradiation, which was reversed by upregulated MUC1.
This study demonstrates that up-regulation of miR-136 induces apoptosis and radiosensitivity of ESCC cells by inhibiting the expression of MUC1.</description><subject>Apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Apoptosis - radiation effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - genetics</subject><subject>Cell Proliferation - radiation effects</subject><subject>Cell Survival - genetics</subject><subject>Cell Survival - radiation effects</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - radiotherapy</subject><subject>Esophageal squamous cell carcinoma</subject><subject>Esophageal Squamous Cell Carcinoma - genetics</subject><subject>Esophageal Squamous Cell Carcinoma - radiotherapy</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic - radiation effects</subject><subject>Humans</subject><subject>Male</subject><subject>microRNA-136</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>MUC1</subject><subject>Mucin-1 - genetics</subject><subject>Radiation Tolerance - genetics</subject><subject>Radiosensitivity</subject><subject>Up-Regulation</subject><subject>X-Rays</subject><issn>0014-4800</issn><issn>1096-0945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2O0zAUhS0EYsrAEyAhL9mk-DqOEy9YjKrhRxpAQnRtOfZN6yqJM3Yymj4I74s7LSxZ-Ufn3OPjj5C3wNbAQH44rI_4OExrzkDlG8Hr5hlZAVOyYEpUz8mKMRCFaBi7Iq9SOjDGFAP-klyVwEXNAVbk93YqIu6W3sw-jDR0dPA2hp_fbwooJfWjWywmaqYwzSH5vBsdjcb5kHBMfvYPfj6ebJjCtDc7ND1N94sZwpKoxb6n1kTrxzCYp2Oi7TFP3fs2e8cdnfdI8XGKmNIl_9t2A6_Ji870Cd9c1muy_XT7a_OluPvx-evm5q6wZaXmooayNkpKXuWOKEtXuQ5AQYWqbGoFrWqUkK0U0rgGuTIgRa7egXVdJaApr8n789wphvsF06wHn07PNCPmAprzikkheAlZWp6l-XdSitjpKfrBxKMGpk889EE_8dAnHvrMI7veXQKWdkD3z_MXQBZ8PAsw13zwGHWyHkeLzke0s3bB_zfgD3t8nfE</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Huang, Hua-Zhong</creator><creator>Yin, Yi-Fa</creator><creator>Wan, Wen-Jun</creator><creator>Xia, Dan</creator><creator>Wang, Rui</creator><creator>Shen, Xue-Min</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201910</creationdate><title>Up-regulation of microRNA-136 induces apoptosis and radiosensitivity of esophageal squamous cell carcinoma cells by inhibiting the expression of MUC1</title><author>Huang, Hua-Zhong ; Yin, Yi-Fa ; Wan, Wen-Jun ; Xia, Dan ; Wang, Rui ; Shen, Xue-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-7137a96625800e63d5df11915e938791b98946b646ad8e29a164312f1cdf54183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Apoptosis - genetics</topic><topic>Apoptosis - radiation effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - genetics</topic><topic>Cell Proliferation - radiation effects</topic><topic>Cell Survival - genetics</topic><topic>Cell Survival - radiation effects</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Neoplasms - radiotherapy</topic><topic>Esophageal squamous cell carcinoma</topic><topic>Esophageal Squamous Cell Carcinoma - genetics</topic><topic>Esophageal Squamous Cell Carcinoma - radiotherapy</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic - radiation effects</topic><topic>Humans</topic><topic>Male</topic><topic>microRNA-136</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>MUC1</topic><topic>Mucin-1 - genetics</topic><topic>Radiation Tolerance - genetics</topic><topic>Radiosensitivity</topic><topic>Up-Regulation</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Hua-Zhong</creatorcontrib><creatorcontrib>Yin, Yi-Fa</creatorcontrib><creatorcontrib>Wan, Wen-Jun</creatorcontrib><creatorcontrib>Xia, Dan</creatorcontrib><creatorcontrib>Wang, Rui</creatorcontrib><creatorcontrib>Shen, Xue-Min</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental and molecular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Hua-Zhong</au><au>Yin, Yi-Fa</au><au>Wan, Wen-Jun</au><au>Xia, Dan</au><au>Wang, Rui</au><au>Shen, Xue-Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Up-regulation of microRNA-136 induces apoptosis and radiosensitivity of esophageal squamous cell carcinoma cells by inhibiting the expression of MUC1</atitle><jtitle>Experimental and molecular pathology</jtitle><addtitle>Exp Mol Pathol</addtitle><date>2019-10</date><risdate>2019</risdate><volume>110</volume><spage>104278</spage><epage>104278</epage><pages>104278-104278</pages><artnum>104278</artnum><issn>0014-4800</issn><eissn>1096-0945</eissn><abstract>This present study is performed to figure out the role of microRNA-136 (miR-136) in radiosensitivity of esophageal squamous cell carcinoma (ESCC) cells through the regulation of MUC1.
Seventy-four ESCC patients were divided into radiotherapy sensitive group and radiotherapy resistance group. Colony formation assay and flow cytometry were used to test the radiosensitivity of radiotherapy resistant strain and parent strain. The expression of miR-136 between radiotherapy resistant strain and parent strain was detected by RT-qPCR, and the expression of miR-136 in Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells was detected after different doses of X-ray irradiation. Eca109 and TE-1 cells as well as Eca109-R and TE-1-R cells with overexpression of miR-136 or co-overexpression of miR-136 and MUC1 were constructed. Cell proliferation, colony formation and apoptosis was detected by CCK-8 assay, colony formation assay, and flow cytometry, respectively.
The expression of miR-136 in ESCC tissues was lower and MUC1 mRNA and protein expression was higher than that in adjacent normal tissues. The expression of miR-136 was negatively correlated with the expression of MUC1 mRNA in ESCC. Low expression of miR-136 and high expression of MUC1 were associated with tumor size, lymph node metastasis and distant metastasis. The expression of miR-136 increased while the expression of MUC1 decreased in the radiotherapy sensitive group of ESCC patients relative to the radiotherapy resistant group. The colony formation ability of radiation resistant cell line was stronger than that of parent cell line, and the apoptosis rate showed an opposite trend. Up-regulation of miR-136 reduced the survival rate, suppressed colony formation ability and induced apoptosis of ESCC cells under irradiation, which was reversed by upregulated MUC1.
This study demonstrates that up-regulation of miR-136 induces apoptosis and radiosensitivity of ESCC cells by inhibiting the expression of MUC1.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>31247211</pmid><doi>10.1016/j.yexmp.2019.104278</doi><tpages>1</tpages></addata></record> |
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| subjects | Apoptosis Apoptosis - genetics Apoptosis - radiation effects Cell Line, Tumor Cell Proliferation - genetics Cell Proliferation - radiation effects Cell Survival - genetics Cell Survival - radiation effects Esophageal Neoplasms - genetics Esophageal Neoplasms - radiotherapy Esophageal squamous cell carcinoma Esophageal Squamous Cell Carcinoma - genetics Esophageal Squamous Cell Carcinoma - radiotherapy Female Gene Expression Regulation, Neoplastic - radiation effects Humans Male microRNA-136 MicroRNAs - genetics Middle Aged MUC1 Mucin-1 - genetics Radiation Tolerance - genetics Radiosensitivity Up-Regulation X-Rays |
| title | Up-regulation of microRNA-136 induces apoptosis and radiosensitivity of esophageal squamous cell carcinoma cells by inhibiting the expression of MUC1 |
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