Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells
Chronic inflammation plays a prominent role in cancer initiation and development. On the other hand, the Inflammation can be established by a number of factors such as viral infections. Parvovirus B19 (B19V) is a pathogen with widespread infection, which infects bone marrow erythroid progenitor cell...
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| Veröffentlicht in: | Biochemical genetics 2020-02, Vol.58 (1), p.63-73 |
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| creator | Amiri, Shahin Atashi, Amir Azad, Mehdi Elmi, Azin Abbaszade Dibavar, Mahnoosh Ajami, Monireh Ajami, Mansoureh Rassaei, Neda Mohammadihaji, Razieh Gheibi, Nematollah |
| description | Chronic inflammation plays a prominent role in cancer initiation and development. On the other hand, the Inflammation can be established by a number of factors such as viral infections. Parvovirus B19 (B19V) is a pathogen with widespread infection, which infects bone marrow erythroid progenitor cells. It has been shown that B19V can also enter human bone marrow mesenchymal stem cells (BM-MSCs). In this study, we hypothesized that BM-MSCs as the main cellular component of bone marrow niche may be induced to secret pro-inflammatory cytokines after B19V infection. BM-MSCs were cultured up to passage 3. The cells were then subjected to nucleofection to transfer a plasmid containing B19V genome. After 36 h, total RNA was extracted and the expression levels of IL-1β, IL-6, TNF-α and NF-κB genes were examined using qRT-PCR. Data analysis showed the significant increase in expression levels of all studied genes in the B19V-transfected cells (
P
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| doi_str_mv | 10.1007/s10528-019-09928-y |
| format | Article |
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P
< 0.05). Although further researches are required, our findings for the first time suggest the importance of B19V infection to establish an inflammatory microenvironment in the bone marrow and its involvement in inflammation-related diseases. Finally, based on our results, molecular assay to diagnose B19V infection of BM-MSCs prior to stem cell therapy is strongly recommended.</description><identifier>ISSN: 0006-2928</identifier><identifier>EISSN: 1573-4927</identifier><identifier>DOI: 10.1007/s10528-019-09928-y</identifier><identifier>PMID: 31250332</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Biotechnology ; Bone marrow ; Cell Line ; Cytokines ; Cytokines - genetics ; Data analysis ; Gene Expression ; Genes ; Genomes ; Human Genetics ; Humans ; IL-1β ; Infections ; Inflammation ; Inflammation - genetics ; Inflammation - virology ; Interleukin 6 ; Medical Microbiology ; Mesenchymal stem cells ; Mesenchymal Stem Cells - virology ; Mesenchyme ; NF-κB protein ; Original Article ; Osteoprogenitor cells ; Parvoviridae Infections - diagnosis ; Parvoviridae Infections - genetics ; Parvoviridae Infections - pathology ; Parvovirus B19, Human ; Parvoviruses ; Ribonucleic acid ; RNA ; Stem cells ; Transfection ; Tumor necrosis factor-α ; Up-Regulation ; Zoology</subject><ispartof>Biochemical genetics, 2020-02, Vol.58 (1), p.63-73</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Biochemical Genetics is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-57a858ac47abf0f922aef4b2df264a51d7d8cb4255a318bfc5d24d360f198e623</citedby><cites>FETCH-LOGICAL-c419t-57a858ac47abf0f922aef4b2df264a51d7d8cb4255a318bfc5d24d360f198e623</cites><orcidid>0000-0001-7503-0894</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10528-019-09928-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10528-019-09928-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31250332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amiri, Shahin</creatorcontrib><creatorcontrib>Atashi, Amir</creatorcontrib><creatorcontrib>Azad, Mehdi</creatorcontrib><creatorcontrib>Elmi, Azin</creatorcontrib><creatorcontrib>Abbaszade Dibavar, Mahnoosh</creatorcontrib><creatorcontrib>Ajami, Monireh</creatorcontrib><creatorcontrib>Ajami, Mansoureh</creatorcontrib><creatorcontrib>Rassaei, Neda</creatorcontrib><creatorcontrib>Mohammadihaji, Razieh</creatorcontrib><creatorcontrib>Gheibi, Nematollah</creatorcontrib><title>Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells</title><title>Biochemical genetics</title><addtitle>Biochem Genet</addtitle><addtitle>Biochem Genet</addtitle><description>Chronic inflammation plays a prominent role in cancer initiation and development. On the other hand, the Inflammation can be established by a number of factors such as viral infections. Parvovirus B19 (B19V) is a pathogen with widespread infection, which infects bone marrow erythroid progenitor cells. It has been shown that B19V can also enter human bone marrow mesenchymal stem cells (BM-MSCs). In this study, we hypothesized that BM-MSCs as the main cellular component of bone marrow niche may be induced to secret pro-inflammatory cytokines after B19V infection. BM-MSCs were cultured up to passage 3. The cells were then subjected to nucleofection to transfer a plasmid containing B19V genome. After 36 h, total RNA was extracted and the expression levels of IL-1β, IL-6, TNF-α and NF-κB genes were examined using qRT-PCR. Data analysis showed the significant increase in expression levels of all studied genes in the B19V-transfected cells (
P
< 0.05). Although further researches are required, our findings for the first time suggest the importance of B19V infection to establish an inflammatory microenvironment in the bone marrow and its involvement in inflammation-related diseases. Finally, based on our results, molecular assay to diagnose B19V infection of BM-MSCs prior to stem cell therapy is strongly recommended.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Cell Line</subject><subject>Cytokines</subject><subject>Cytokines - genetics</subject><subject>Data analysis</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genomes</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>IL-1β</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Inflammation - genetics</subject><subject>Inflammation - virology</subject><subject>Interleukin 6</subject><subject>Medical Microbiology</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - virology</subject><subject>Mesenchyme</subject><subject>NF-κB protein</subject><subject>Original Article</subject><subject>Osteoprogenitor cells</subject><subject>Parvoviridae Infections - diagnosis</subject><subject>Parvoviridae Infections - genetics</subject><subject>Parvoviridae Infections - pathology</subject><subject>Parvovirus B19, Human</subject><subject>Parvoviruses</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Stem cells</subject><subject>Transfection</subject><subject>Tumor necrosis factor-α</subject><subject>Up-Regulation</subject><subject>Zoology</subject><issn>0006-2928</issn><issn>1573-4927</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1LxDAQhoMouq7-AQ8S8OKlms-2OeriFyguqOeQtolWm2RNWqX_3uiuCh48TYZ55p3JvADsYXSEESqOI0aclBnCIkNCpNe4BiaYFzRjghTrYIIQyjOSKltgO8bnlArE2CbYophwRCmZAPuwCPpx6FTfege9gfPgs9aZTlmreh9GOBt7_9I6DS-00xFWI5yr8Obf2jBEeIoFbB28HKxy8NQn6kaF4N_hjY7a1U-jVR2867WFM911cQdsGNVFvbuKU_BwfnY_u8yuby-uZifXWc2w6DNeqJKXqmaFqgwyghClDatIY0jOFMdN0ZR1xQjniuKyMjVvCGtojgwWpc4JnYLDpe4i-NdBx17aNtZpA-W0H6Ik6fs5zYt0hCk4-IM--yG4tF2iWImJyHOaKLKk6uBjDNrIRWitCqPESH6aIZdmyGSG_DJDjqlpfyU9VFY3Py3f108AXQIxldyjDr-z_5H9ADqPlVI</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Amiri, Shahin</creator><creator>Atashi, Amir</creator><creator>Azad, Mehdi</creator><creator>Elmi, Azin</creator><creator>Abbaszade Dibavar, Mahnoosh</creator><creator>Ajami, Monireh</creator><creator>Ajami, Mansoureh</creator><creator>Rassaei, Neda</creator><creator>Mohammadihaji, Razieh</creator><creator>Gheibi, Nematollah</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7503-0894</orcidid></search><sort><creationdate>20200201</creationdate><title>Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells</title><author>Amiri, Shahin ; Atashi, Amir ; Azad, Mehdi ; Elmi, Azin ; Abbaszade Dibavar, Mahnoosh ; Ajami, Monireh ; Ajami, Mansoureh ; Rassaei, Neda ; Mohammadihaji, Razieh ; Gheibi, Nematollah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-57a858ac47abf0f922aef4b2df264a51d7d8cb4255a318bfc5d24d360f198e623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Cell Line</topic><topic>Cytokines</topic><topic>Cytokines - 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Academic</collection><jtitle>Biochemical genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amiri, Shahin</au><au>Atashi, Amir</au><au>Azad, Mehdi</au><au>Elmi, Azin</au><au>Abbaszade Dibavar, Mahnoosh</au><au>Ajami, Monireh</au><au>Ajami, Mansoureh</au><au>Rassaei, Neda</au><au>Mohammadihaji, Razieh</au><au>Gheibi, Nematollah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells</atitle><jtitle>Biochemical genetics</jtitle><stitle>Biochem Genet</stitle><addtitle>Biochem Genet</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>58</volume><issue>1</issue><spage>63</spage><epage>73</epage><pages>63-73</pages><issn>0006-2928</issn><eissn>1573-4927</eissn><abstract>Chronic inflammation plays a prominent role in cancer initiation and development. On the other hand, the Inflammation can be established by a number of factors such as viral infections. Parvovirus B19 (B19V) is a pathogen with widespread infection, which infects bone marrow erythroid progenitor cells. It has been shown that B19V can also enter human bone marrow mesenchymal stem cells (BM-MSCs). In this study, we hypothesized that BM-MSCs as the main cellular component of bone marrow niche may be induced to secret pro-inflammatory cytokines after B19V infection. BM-MSCs were cultured up to passage 3. The cells were then subjected to nucleofection to transfer a plasmid containing B19V genome. After 36 h, total RNA was extracted and the expression levels of IL-1β, IL-6, TNF-α and NF-κB genes were examined using qRT-PCR. Data analysis showed the significant increase in expression levels of all studied genes in the B19V-transfected cells (
P
< 0.05). Although further researches are required, our findings for the first time suggest the importance of B19V infection to establish an inflammatory microenvironment in the bone marrow and its involvement in inflammation-related diseases. Finally, based on our results, molecular assay to diagnose B19V infection of BM-MSCs prior to stem cell therapy is strongly recommended.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>31250332</pmid><doi>10.1007/s10528-019-09928-y</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-7503-0894</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Biotechnology Bone marrow Cell Line Cytokines Cytokines - genetics Data analysis Gene Expression Genes Genomes Human Genetics Humans IL-1β Infections Inflammation Inflammation - genetics Inflammation - virology Interleukin 6 Medical Microbiology Mesenchymal stem cells Mesenchymal Stem Cells - virology Mesenchyme NF-κB protein Original Article Osteoprogenitor cells Parvoviridae Infections - diagnosis Parvoviridae Infections - genetics Parvoviridae Infections - pathology Parvovirus B19, Human Parvoviruses Ribonucleic acid RNA Stem cells Transfection Tumor necrosis factor-α Up-Regulation Zoology |
| title | Upregulation of Pro-inflammatory Cytokine Genes by Parvovirus B19 in Human Bone Marrow Mesenchymal Stem Cells |
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