Molecular composition of the paralyzing venom of three solitary wasps (Hymenoptera: Pompilidae) collected in southeast Mexico
The chemical and biological characterization of peptide and protein components of the paralyzing venom from three Pompilidae solitary spider wasps (Pepsis mexicana, Pepsis terminata, and Anoplius nigritus) is described for the first time. The molecular masses of the most abundant peptides were deter...
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| Veröffentlicht in: | Toxicon (Oxford) 2019-10, Vol.168, p.98-102 |
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| container_title | Toxicon (Oxford) |
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| creator | Huicab-Uribe, M.A. Verdel-Aranda, K. Martínez-Hernández, A. Zamudio, F.Z. Jiménez-Vargas, J.M. Lara-Reyna, J. |
| description | The chemical and biological characterization of peptide and protein components of the paralyzing venom from three Pompilidae solitary spider wasps (Pepsis mexicana, Pepsis terminata, and Anoplius nigritus) is described for the first time. The molecular masses of the most abundant peptides were determined. The N-terminal sequences of two cysteine-rich peptides were obtained from Pepsis. Metalloproteinase and hyaluronidase activities were identified in the venom of P. mexicana. A novel non-lethal method to collect venom is described.
•The molecular characterization of venom from three Pompilidae is described for first time.•The peptide chromatographic profiles of the three species are different.•Peptides similar to Bradykinin or rich in cysteine were found.•P. mexicana venom presents metalloprotease and hyaluronidase activities.•A novel non-lethal method to collect spider wasp venom is described. |
| doi_str_mv | 10.1016/j.toxicon.2019.06.224 |
| format | Article |
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•The molecular characterization of venom from three Pompilidae is described for first time.•The peptide chromatographic profiles of the three species are different.•Peptides similar to Bradykinin or rich in cysteine were found.•P. mexicana venom presents metalloprotease and hyaluronidase activities.•A novel non-lethal method to collect spider wasp venom is described.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/j.toxicon.2019.06.224</identifier><identifier>PMID: 31251992</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Enzymatic activities ; Female ; Hyaluronoglucosaminidase - analysis ; Insect Proteins - chemistry ; Metalloproteases - analysis ; Mexico ; Peptide profile ; Pompilidae ; Solitary spider wasp ; Venom composition ; Wasp Venoms - chemistry ; Wasp Venoms - enzymology ; Wasps</subject><ispartof>Toxicon (Oxford), 2019-10, Vol.168, p.98-102</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-3afd023fea78ce736d62771489bbe8fd4cbccd58de4ada6ff94535ef43d6295f3</citedby><cites>FETCH-LOGICAL-c365t-3afd023fea78ce736d62771489bbe8fd4cbccd58de4ada6ff94535ef43d6295f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S004101011930399X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31251992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huicab-Uribe, M.A.</creatorcontrib><creatorcontrib>Verdel-Aranda, K.</creatorcontrib><creatorcontrib>Martínez-Hernández, A.</creatorcontrib><creatorcontrib>Zamudio, F.Z.</creatorcontrib><creatorcontrib>Jiménez-Vargas, J.M.</creatorcontrib><creatorcontrib>Lara-Reyna, J.</creatorcontrib><title>Molecular composition of the paralyzing venom of three solitary wasps (Hymenoptera: Pompilidae) collected in southeast Mexico</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>The chemical and biological characterization of peptide and protein components of the paralyzing venom from three Pompilidae solitary spider wasps (Pepsis mexicana, Pepsis terminata, and Anoplius nigritus) is described for the first time. The molecular masses of the most abundant peptides were determined. The N-terminal sequences of two cysteine-rich peptides were obtained from Pepsis. Metalloproteinase and hyaluronidase activities were identified in the venom of P. mexicana. A novel non-lethal method to collect venom is described.
•The molecular characterization of venom from three Pompilidae is described for first time.•The peptide chromatographic profiles of the three species are different.•Peptides similar to Bradykinin or rich in cysteine were found.•P. mexicana venom presents metalloprotease and hyaluronidase activities.•A novel non-lethal method to collect spider wasp venom is described.</description><subject>Animals</subject><subject>Enzymatic activities</subject><subject>Female</subject><subject>Hyaluronoglucosaminidase - analysis</subject><subject>Insect Proteins - chemistry</subject><subject>Metalloproteases - analysis</subject><subject>Mexico</subject><subject>Peptide profile</subject><subject>Pompilidae</subject><subject>Solitary spider wasp</subject><subject>Venom composition</subject><subject>Wasp Venoms - chemistry</subject><subject>Wasp Venoms - enzymology</subject><subject>Wasps</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v1DAQxS0EotvCRwD5WA4J_hM7CRdUVZQitYIDnC2vPQavnDjYTmGR-O54tQtXTiONfu-9mYfQC0paSqh8vWtL_OlNnFtG6NgS2TLWPUIbOvRjw6kgj9GGkI42pOJn6DznHSGED6N8is44ZYKOI9ug3_cxgFmDTtjEaYnZFx9nHB0u3wAvOumw_-Xnr_gB5jgd9wkA5xh80WmPf-i8ZHx5u58qsBRI-g3-VJ188FbDq-oaakABi_1cVWu11bngezgc_ww9cTpkeH6aF-jLzbvP17fN3cf3H66v7hrDpSgN184Sxh3ofjDQc2kl63vaDeN2C4OzndkaY8VgodNWS-fGTnABruMVHIXjF-jy6Luk-H2FXNTks4EQ9AxxzYoxQSSnlAwVFUfUpJhzAqeW5Kf6qaJEHZpXO3VqXh2aV0RWeVd1L08R63YC-0_1t-oKvD0CUB998JBUNh5mA9anWpCy0f8n4g-3xZtV</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Huicab-Uribe, M.A.</creator><creator>Verdel-Aranda, K.</creator><creator>Martínez-Hernández, A.</creator><creator>Zamudio, F.Z.</creator><creator>Jiménez-Vargas, J.M.</creator><creator>Lara-Reyna, J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201910</creationdate><title>Molecular composition of the paralyzing venom of three solitary wasps (Hymenoptera: Pompilidae) collected in southeast Mexico</title><author>Huicab-Uribe, M.A. ; Verdel-Aranda, K. ; Martínez-Hernández, A. ; Zamudio, F.Z. ; Jiménez-Vargas, J.M. ; Lara-Reyna, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-3afd023fea78ce736d62771489bbe8fd4cbccd58de4ada6ff94535ef43d6295f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Enzymatic activities</topic><topic>Female</topic><topic>Hyaluronoglucosaminidase - analysis</topic><topic>Insect Proteins - chemistry</topic><topic>Metalloproteases - analysis</topic><topic>Mexico</topic><topic>Peptide profile</topic><topic>Pompilidae</topic><topic>Solitary spider wasp</topic><topic>Venom composition</topic><topic>Wasp Venoms - chemistry</topic><topic>Wasp Venoms - enzymology</topic><topic>Wasps</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huicab-Uribe, M.A.</creatorcontrib><creatorcontrib>Verdel-Aranda, K.</creatorcontrib><creatorcontrib>Martínez-Hernández, A.</creatorcontrib><creatorcontrib>Zamudio, F.Z.</creatorcontrib><creatorcontrib>Jiménez-Vargas, J.M.</creatorcontrib><creatorcontrib>Lara-Reyna, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huicab-Uribe, M.A.</au><au>Verdel-Aranda, K.</au><au>Martínez-Hernández, A.</au><au>Zamudio, F.Z.</au><au>Jiménez-Vargas, J.M.</au><au>Lara-Reyna, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular composition of the paralyzing venom of three solitary wasps (Hymenoptera: Pompilidae) collected in southeast Mexico</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>2019-10</date><risdate>2019</risdate><volume>168</volume><spage>98</spage><epage>102</epage><pages>98-102</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><abstract>The chemical and biological characterization of peptide and protein components of the paralyzing venom from three Pompilidae solitary spider wasps (Pepsis mexicana, Pepsis terminata, and Anoplius nigritus) is described for the first time. The molecular masses of the most abundant peptides were determined. The N-terminal sequences of two cysteine-rich peptides were obtained from Pepsis. Metalloproteinase and hyaluronidase activities were identified in the venom of P. mexicana. A novel non-lethal method to collect venom is described.
•The molecular characterization of venom from three Pompilidae is described for first time.•The peptide chromatographic profiles of the three species are different.•Peptides similar to Bradykinin or rich in cysteine were found.•P. mexicana venom presents metalloprotease and hyaluronidase activities.•A novel non-lethal method to collect spider wasp venom is described.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31251992</pmid><doi>10.1016/j.toxicon.2019.06.224</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Enzymatic activities Female Hyaluronoglucosaminidase - analysis Insect Proteins - chemistry Metalloproteases - analysis Mexico Peptide profile Pompilidae Solitary spider wasp Venom composition Wasp Venoms - chemistry Wasp Venoms - enzymology Wasps |
| title | Molecular composition of the paralyzing venom of three solitary wasps (Hymenoptera: Pompilidae) collected in southeast Mexico |
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