Laminin‐derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells
Laminin peptides influence cancer biology. We investigated the role of a laminin‐derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane‐type matrix m...
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| Veröffentlicht in: | Journal of cellular physiology 2020-01, Vol.235 (1), p.587-598 |
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| creator | Caires‐dos‐Santos, Livia da Silva, Suély V. Smuczek, Basilio Siqueira, Adriane S. Cruz, Karen S. P. Barbuto, José Alexandre M. Augusto, Taize M. Freitas, Vanessa M. Carvalho, Hernandes F. Jaeger, Ruy G. |
| description | Laminin peptides influence cancer biology. We investigated the role of a laminin‐derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane‐type matrix metalloproteinase 1. Reactive oxygen species generation is also related to invadopodia formation. This prompted us to address whether C16 would induce reactive oxygen species generation in DU145 cells. Quantitative fluorescence and flow cytometry showed that the peptide C16 increased reactive oxygen species in DU145 cells. Furthermore, significant colocalization between Tks5 and reactive oxygen species was observed in C16‐treated cells. Results suggested that the peptide C16 increased Tks5 and reactive oxygen species in prostate cancer cells. The role of C16 increasing Tks and reactive oxygen species are novel findings on invadopodia activity.
C16 regulates Tks and ROS in cancer invadopodia |
| doi_str_mv | 10.1002/jcp.28997 |
| format | Article |
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C16 regulates Tks and ROS in cancer invadopodia</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.28997</identifier><identifier>PMID: 31254281</identifier><language>eng</language><publisher>HOBOKEN: Wiley</publisher><subject>Cell Biology ; extracellular matrix ; Flow cytometry ; Fluorescence ; invadopodia ; Laminin ; Life Sciences & Biomedicine ; Matrix metalloproteinase ; Matrix metalloproteinases ; Metalloproteinase ; Oxygen ; Peptides ; Physiology ; Prostate cancer ; Reactive oxygen species ; Science & Technology ; Tks4 ; Tks5</subject><ispartof>Journal of cellular physiology, 2020-01, Vol.235 (1), p.587-598</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>9</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000476131200001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c3537-e185d35bdaae8d2450e96c6949f35480c811295856b927b05e29241f47c5d2893</citedby><cites>FETCH-LOGICAL-c3537-e185d35bdaae8d2450e96c6949f35480c811295856b927b05e29241f47c5d2893</cites><orcidid>0000-0002-1564-0356 ; 0000-0002-3080-9447 ; 0000-0001-9613-8626 ; 0000-0001-9526-6781</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.28997$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.28997$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,28257,45583,45584</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31254281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caires‐dos‐Santos, Livia</creatorcontrib><creatorcontrib>da Silva, Suély V.</creatorcontrib><creatorcontrib>Smuczek, Basilio</creatorcontrib><creatorcontrib>Siqueira, Adriane S.</creatorcontrib><creatorcontrib>Cruz, Karen S. P.</creatorcontrib><creatorcontrib>Barbuto, José Alexandre M.</creatorcontrib><creatorcontrib>Augusto, Taize M.</creatorcontrib><creatorcontrib>Freitas, Vanessa M.</creatorcontrib><creatorcontrib>Carvalho, Hernandes F.</creatorcontrib><creatorcontrib>Jaeger, Ruy G.</creatorcontrib><title>Laminin‐derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells</title><title>Journal of cellular physiology</title><addtitle>J CELL PHYSIOL</addtitle><addtitle>J Cell Physiol</addtitle><description>Laminin peptides influence cancer biology. We investigated the role of a laminin‐derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane‐type matrix metalloproteinase 1. Reactive oxygen species generation is also related to invadopodia formation. This prompted us to address whether C16 would induce reactive oxygen species generation in DU145 cells. Quantitative fluorescence and flow cytometry showed that the peptide C16 increased reactive oxygen species in DU145 cells. Furthermore, significant colocalization between Tks5 and reactive oxygen species was observed in C16‐treated cells. Results suggested that the peptide C16 increased Tks5 and reactive oxygen species in prostate cancer cells. The role of C16 increasing Tks and reactive oxygen species are novel findings on invadopodia activity.
C16 regulates Tks and ROS in cancer invadopodia</description><subject>Cell Biology</subject><subject>extracellular matrix</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>invadopodia</subject><subject>Laminin</subject><subject>Life Sciences & Biomedicine</subject><subject>Matrix metalloproteinase</subject><subject>Matrix metalloproteinases</subject><subject>Metalloproteinase</subject><subject>Oxygen</subject><subject>Peptides</subject><subject>Physiology</subject><subject>Prostate cancer</subject><subject>Reactive oxygen species</subject><subject>Science & Technology</subject><subject>Tks4</subject><subject>Tks5</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><recordid>eNqNkc1u1DAUhS0EokNhwQsgS2xAKK1_k3hZReVPI8GirCPHuSkeEifYCe1ILHgEnpEn4U5n2kUlJFa-0v3O1fE5hDzn7IQzJk43bjoRpTHFA7LizBSZyrV4SFa445nRih-RJyltGGPGSPmYHEkutBIlX5Gfazv44MOfX79biP4HtHSCafYt0IrnNMLl0tsZEr34lihcTxFS8mOgNrS4tG5GCR2vt5cQaJrAeURxhmjnHeYD_boMNtApjmnGQ9TZ4CBSB32fnpJHne0TPDu8x-TL2_OL6n22_vTuQ3W2zpzUssiAl7qVummthbIVSjMwucuNMp3UqmSu5FwYXeq8MaJomAZhhOKdKpxuMRd5TF7t76KL7wukuR582jmwAcYl1UJolgtT6ALRl_fQzbjEgO5qIRlnWitZIvV6Tzn8VorQ1VP0g43bmrN6V0mNldQ3lSD74nBxaQZo78jbDhAo98AVNGOXMEJM6A7D0lSRc6RxYrzy802y1biEGaVv_l-K9OmB9j1s_225_lh93nv_CzKQttU</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Caires‐dos‐Santos, Livia</creator><creator>da Silva, Suély V.</creator><creator>Smuczek, Basilio</creator><creator>Siqueira, Adriane S.</creator><creator>Cruz, Karen S. P.</creator><creator>Barbuto, José Alexandre M.</creator><creator>Augusto, Taize M.</creator><creator>Freitas, Vanessa M.</creator><creator>Carvalho, Hernandes F.</creator><creator>Jaeger, Ruy G.</creator><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1564-0356</orcidid><orcidid>https://orcid.org/0000-0002-3080-9447</orcidid><orcidid>https://orcid.org/0000-0001-9613-8626</orcidid><orcidid>https://orcid.org/0000-0001-9526-6781</orcidid></search><sort><creationdate>202001</creationdate><title>Laminin‐derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells</title><author>Caires‐dos‐Santos, Livia ; da Silva, Suély V. ; Smuczek, Basilio ; Siqueira, Adriane S. ; Cruz, Karen S. P. ; Barbuto, José Alexandre M. ; Augusto, Taize M. ; Freitas, Vanessa M. ; Carvalho, Hernandes F. ; Jaeger, Ruy G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-e185d35bdaae8d2450e96c6949f35480c811295856b927b05e29241f47c5d2893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Cell Biology</topic><topic>extracellular matrix</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>invadopodia</topic><topic>Laminin</topic><topic>Life Sciences & Biomedicine</topic><topic>Matrix metalloproteinase</topic><topic>Matrix metalloproteinases</topic><topic>Metalloproteinase</topic><topic>Oxygen</topic><topic>Peptides</topic><topic>Physiology</topic><topic>Prostate cancer</topic><topic>Reactive oxygen species</topic><topic>Science & Technology</topic><topic>Tks4</topic><topic>Tks5</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caires‐dos‐Santos, Livia</creatorcontrib><creatorcontrib>da Silva, Suély V.</creatorcontrib><creatorcontrib>Smuczek, Basilio</creatorcontrib><creatorcontrib>Siqueira, Adriane S.</creatorcontrib><creatorcontrib>Cruz, Karen S. 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P.</au><au>Barbuto, José Alexandre M.</au><au>Augusto, Taize M.</au><au>Freitas, Vanessa M.</au><au>Carvalho, Hernandes F.</au><au>Jaeger, Ruy G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laminin‐derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells</atitle><jtitle>Journal of cellular physiology</jtitle><stitle>J CELL PHYSIOL</stitle><addtitle>J Cell Physiol</addtitle><date>2020-01</date><risdate>2020</risdate><volume>235</volume><issue>1</issue><spage>587</spage><epage>598</epage><pages>587-598</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Laminin peptides influence cancer biology. We investigated the role of a laminin‐derived peptide C16 regulating invadopodia molecules in human prostate cancer cells (DU145). C16 augmented invadopodia activity of DU145 cells, and stimulated expression Tks4, Tks5, cortactin, and membrane‐type matrix metalloproteinase 1. Reactive oxygen species generation is also related to invadopodia formation. This prompted us to address whether C16 would induce reactive oxygen species generation in DU145 cells. Quantitative fluorescence and flow cytometry showed that the peptide C16 increased reactive oxygen species in DU145 cells. Furthermore, significant colocalization between Tks5 and reactive oxygen species was observed in C16‐treated cells. Results suggested that the peptide C16 increased Tks5 and reactive oxygen species in prostate cancer cells. The role of C16 increasing Tks and reactive oxygen species are novel findings on invadopodia activity.
C16 regulates Tks and ROS in cancer invadopodia</abstract><cop>HOBOKEN</cop><pub>Wiley</pub><pmid>31254281</pmid><doi>10.1002/jcp.28997</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-1564-0356</orcidid><orcidid>https://orcid.org/0000-0002-3080-9447</orcidid><orcidid>https://orcid.org/0000-0001-9613-8626</orcidid><orcidid>https://orcid.org/0000-0001-9526-6781</orcidid></addata></record> |
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| subjects | Cell Biology extracellular matrix Flow cytometry Fluorescence invadopodia Laminin Life Sciences & Biomedicine Matrix metalloproteinase Matrix metalloproteinases Metalloproteinase Oxygen Peptides Physiology Prostate cancer Reactive oxygen species Science & Technology Tks4 Tks5 |
| title | Laminin‐derived peptide C16 regulates Tks expression and reactive oxygen species generation in human prostate cancer cells |
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