Differences in levan nanoparticles depending on their synthesis route: Microbial vs cell-free systems

Differences between the levan obtained from bacteria and from cell-free systems were studied in this work. Results showed that both polymers are non-porous solids (type II isotherm with 20 m2/g) with a main thermal decomposition at 200 °C and a negligible value of protein adsorption. Microbial levan...

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Veröffentlicht in:International journal of biological macromolecules 2019-09, Vol.137, p.62-68
Hauptverfasser: González-Garcinuño, Álvaro, Tabernero, Antonio, Marcelo, Gema, Sebastián, Víctor, Arruebo, Manuel, Santamaría, Jesús, Martín del Valle, Eva
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container_title International journal of biological macromolecules
container_volume 137
creator González-Garcinuño, Álvaro
Tabernero, Antonio
Marcelo, Gema
Sebastián, Víctor
Arruebo, Manuel
Santamaría, Jesús
Martín del Valle, Eva
description Differences between the levan obtained from bacteria and from cell-free systems were studied in this work. Results showed that both polymers are non-porous solids (type II isotherm with 20 m2/g) with a main thermal decomposition at 200 °C and a negligible value of protein adsorption. Microbial levan produced nanoparticles of 90 nm in diameter whereas nanoparticles of 110 nm were obtained with the polymer obtained from a cell-free system. Both polymers behave as aggregates depending on the critical aggregation concentration. At the same time, that concentration depends on the technique used for the polymer synthesis. Cell-free system aggregation concentration is 0.24 mg/mL whereas a concentration of 0.05 mg/mL was found for the microbial system. In both cases, the average molecular weight of the aggregate is higher than 2000 kDa. These results highlight the existence of aggregation equilibrium for both polymers that has to be taken into account for future applications. •Polyfructoses CAC is depending on the synthesis route.•Differences in MWs were observed at concentrations above the CAC.•Polymer thermal analysis is not dependent on the synthesis route.•Both synthesis routes provided non-porous polymers.•Stable and spherical nanoparticles were obtained for both systems.•Nanoparticles showed a low non-specific adsorption.
doi_str_mv 10.1016/j.ijbiomac.2019.06.128
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subjects Acinetobacter - metabolism
Cell-Free System - metabolism
Fructans - biosynthesis
Fructans - chemistry
Nanoparticles
Nanoparticles - chemistry
Particle Size
Polyfructoses
Self-assembly
title Differences in levan nanoparticles depending on their synthesis route: Microbial vs cell-free systems
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