ToyA, a positive pathway-specific regulator for toyocamycin biosynthesis in Streptomyces diastatochromogenes 1628
The nucleoside antibiotic toyocamycin (TM), which was produced by Streptomyces diastatochromogenes 1628, was found to be highly efficient against a broad range of plant pathogenic fungi. Despite its importance, little is known about the regulation TM biosynthesis. In this study, toyA , located in th...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2019-09, Vol.103 (17), p.7071-7084 |
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| creator | Xu, Jie Song, Zhangqing Xu, Xianhao Ma, Zheng Bechthold, Andreas Yu, Xiaoping |
| description | The nucleoside antibiotic toyocamycin (TM), which was produced by
Streptomyces diastatochromogenes
1628, was found to be highly efficient against a broad range of plant pathogenic fungi. Despite its importance, little is known about the regulation TM biosynthesis. In this study,
toyA
, located in the TM biosynthetic gene cluster, was identified as a regulatory gene encoding a large ATP-binding regulator of the LuxR family (LAL-family). The role of
toyA
in TM biosynthesis in
S. diastatochromogenes
1628 was investigated by gene deletion, complementation, and over-expression. Gene disruption of
toyA
resulted in almost loss of TM production. TM production in complemented strain was restored to the level comparable to that in the wild-type strain
S. diastatochromogenes
1628. Over-expression of
toyA
separately controlled by promoter SPL57, SPL21, and p
erm
E
*
in wild-type strain
S. diastatochromogenes
1628 led to a 2-fold, 1-fold, and 80% increase in TM production compared with wild-type strain
S. diastatochromogenes
1628, respectively. Quantitative RT-PCR analysis revealed that the transcriptional level of
toy
structural genes was downregulated in the ΔtoyA mutant but restored in complemented strain and further upregulated in the
toyA
over-expression strain. The detection results from GFP reporter system in
Escherichia coli
and GUS reporter system and GUS activities in
S. albus
J1074 and
S. diastatochromogenes
1628 showed that ToyA activated the expression of
toyB
and
toyE
operon directly and activated the expression of other
toy
structural genes indirectly. These results indicate that ToyA is essential for TM biosynthesis controlling the expression of structural genes. |
| doi_str_mv | 10.1007/s00253-019-09959-w |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2250621508</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A596346810</galeid><sourcerecordid>A596346810</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-db6cb46da165067995b071932b90e8c009bedc66cdca7a95126d82ddf51b764e3</originalsourceid><addsrcrecordid>eNp9kl-L1TAQxYso7nX1C_ggBV8U7DpJm7R5vCz-WVgQ3PU5pMm0N0vbdJPUa7-9We_qckUkhDAzv3PIwMmylwTOCED9PgBQVhZARAFCMFHsH2UbUpW0AE6qx9kGSM2KmonmJHsWwg0AoQ3nT7OTklDGKW022e21W7fvcpXPLthov2M-q7jbq7UIM2rbWZ177JdBRefzLt3oVqfVuGo75a11YZ3iDoMNeaqvosc5ujTEkBurQkwyvfNudD1OqUc4bZ5nTzo1BHxx_55m3z5-uD7_XFx--XRxvr0sNCNlLEzLdVtxowhnwOu0Xws1ESVtBWCjAUSLRnOujVa1EoxQbhpqTMdIW_MKy9PszcF39u52wRDlaIPGYVATuiVISpMvJQyahL7-C71xi5_S7xJViZJAQh-oXg0o7dS56JW-M5VbJnhZ8YZAos7-QaVjcLTaTdjZ1D8SvD0SJCbij9irJQR5cfX1mKUHVnsXgsdOzt6Oyq-SgLzLhDxkQqZMyF-ZkPskenW_3dKOaP5IfocgAeUBCGk09egf1v-P7U-KhME3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2249310506</pqid></control><display><type>article</type><title>ToyA, a positive pathway-specific regulator for toyocamycin biosynthesis in Streptomyces diastatochromogenes 1628</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Xu, Jie ; Song, Zhangqing ; Xu, Xianhao ; Ma, Zheng ; Bechthold, Andreas ; Yu, Xiaoping</creator><creatorcontrib>Xu, Jie ; Song, Zhangqing ; Xu, Xianhao ; Ma, Zheng ; Bechthold, Andreas ; Yu, Xiaoping</creatorcontrib><description>The nucleoside antibiotic toyocamycin (TM), which was produced by
Streptomyces diastatochromogenes
1628, was found to be highly efficient against a broad range of plant pathogenic fungi. Despite its importance, little is known about the regulation TM biosynthesis. In this study,
toyA
, located in the TM biosynthetic gene cluster, was identified as a regulatory gene encoding a large ATP-binding regulator of the LuxR family (LAL-family). The role of
toyA
in TM biosynthesis in
S. diastatochromogenes
1628 was investigated by gene deletion, complementation, and over-expression. Gene disruption of
toyA
resulted in almost loss of TM production. TM production in complemented strain was restored to the level comparable to that in the wild-type strain
S. diastatochromogenes
1628. Over-expression of
toyA
separately controlled by promoter SPL57, SPL21, and p
erm
E
*
in wild-type strain
S. diastatochromogenes
1628 led to a 2-fold, 1-fold, and 80% increase in TM production compared with wild-type strain
S. diastatochromogenes
1628, respectively. Quantitative RT-PCR analysis revealed that the transcriptional level of
toy
structural genes was downregulated in the ΔtoyA mutant but restored in complemented strain and further upregulated in the
toyA
over-expression strain. The detection results from GFP reporter system in
Escherichia coli
and GUS reporter system and GUS activities in
S. albus
J1074 and
S. diastatochromogenes
1628 showed that ToyA activated the expression of
toyB
and
toyE
operon directly and activated the expression of other
toy
structural genes indirectly. These results indicate that ToyA is essential for TM biosynthesis controlling the expression of structural genes.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-019-09959-w</identifier><identifier>PMID: 31256228</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antibiotics ; Applied Genetics and Molecular Biotechnology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biomedical and Life Sciences ; Biosynthesis ; Biosynthetic Pathways - genetics ; Biotechnology ; Complementation ; Disruption ; E coli ; Escherichia coli ; Fungi ; Gene deletion ; Gene disruption ; Gene Expression ; Gene Expression Regulation, Bacterial ; Genes ; Genetic research ; Genetic transcription ; Life Sciences ; Microbial Genetics and Genomics ; Microbiology ; Multigene Family ; Mutation ; Overexpression ; Physiological aspects ; Polymerase chain reaction ; Promoter Regions, Genetic ; Streptomyces - genetics ; Streptomyces - metabolism ; Streptomyces diastatochromogenes ; Toyocamycin - biosynthesis ; Transcription ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Applied microbiology and biotechnology, 2019-09, Vol.103 (17), p.7071-7084</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-db6cb46da165067995b071932b90e8c009bedc66cdca7a95126d82ddf51b764e3</citedby><cites>FETCH-LOGICAL-c513t-db6cb46da165067995b071932b90e8c009bedc66cdca7a95126d82ddf51b764e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-019-09959-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-019-09959-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31256228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Song, Zhangqing</creatorcontrib><creatorcontrib>Xu, Xianhao</creatorcontrib><creatorcontrib>Ma, Zheng</creatorcontrib><creatorcontrib>Bechthold, Andreas</creatorcontrib><creatorcontrib>Yu, Xiaoping</creatorcontrib><title>ToyA, a positive pathway-specific regulator for toyocamycin biosynthesis in Streptomyces diastatochromogenes 1628</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The nucleoside antibiotic toyocamycin (TM), which was produced by
Streptomyces diastatochromogenes
1628, was found to be highly efficient against a broad range of plant pathogenic fungi. Despite its importance, little is known about the regulation TM biosynthesis. In this study,
toyA
, located in the TM biosynthetic gene cluster, was identified as a regulatory gene encoding a large ATP-binding regulator of the LuxR family (LAL-family). The role of
toyA
in TM biosynthesis in
S. diastatochromogenes
1628 was investigated by gene deletion, complementation, and over-expression. Gene disruption of
toyA
resulted in almost loss of TM production. TM production in complemented strain was restored to the level comparable to that in the wild-type strain
S. diastatochromogenes
1628. Over-expression of
toyA
separately controlled by promoter SPL57, SPL21, and p
erm
E
*
in wild-type strain
S. diastatochromogenes
1628 led to a 2-fold, 1-fold, and 80% increase in TM production compared with wild-type strain
S. diastatochromogenes
1628, respectively. Quantitative RT-PCR analysis revealed that the transcriptional level of
toy
structural genes was downregulated in the ΔtoyA mutant but restored in complemented strain and further upregulated in the
toyA
over-expression strain. The detection results from GFP reporter system in
Escherichia coli
and GUS reporter system and GUS activities in
S. albus
J1074 and
S. diastatochromogenes
1628 showed that ToyA activated the expression of
toyB
and
toyE
operon directly and activated the expression of other
toy
structural genes indirectly. These results indicate that ToyA is essential for TM biosynthesis controlling the expression of structural genes.</description><subject>Antibiotics</subject><subject>Applied Genetics and Molecular Biotechnology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways - genetics</subject><subject>Biotechnology</subject><subject>Complementation</subject><subject>Disruption</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Fungi</subject><subject>Gene deletion</subject><subject>Gene disruption</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetic transcription</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Overexpression</subject><subject>Physiological aspects</subject><subject>Polymerase chain reaction</subject><subject>Promoter Regions, Genetic</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces diastatochromogenes</subject><subject>Toyocamycin - biosynthesis</subject><subject>Transcription</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kl-L1TAQxYso7nX1C_ggBV8U7DpJm7R5vCz-WVgQ3PU5pMm0N0vbdJPUa7-9We_qckUkhDAzv3PIwMmylwTOCED9PgBQVhZARAFCMFHsH2UbUpW0AE6qx9kGSM2KmonmJHsWwg0AoQ3nT7OTklDGKW022e21W7fvcpXPLthov2M-q7jbq7UIM2rbWZ177JdBRefzLt3oVqfVuGo75a11YZ3iDoMNeaqvosc5ujTEkBurQkwyvfNudD1OqUc4bZ5nTzo1BHxx_55m3z5-uD7_XFx--XRxvr0sNCNlLEzLdVtxowhnwOu0Xws1ESVtBWCjAUSLRnOujVa1EoxQbhpqTMdIW_MKy9PszcF39u52wRDlaIPGYVATuiVISpMvJQyahL7-C71xi5_S7xJViZJAQh-oXg0o7dS56JW-M5VbJnhZ8YZAos7-QaVjcLTaTdjZ1D8SvD0SJCbij9irJQR5cfX1mKUHVnsXgsdOzt6Oyq-SgLzLhDxkQqZMyF-ZkPskenW_3dKOaP5IfocgAeUBCGk09egf1v-P7U-KhME3</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Xu, Jie</creator><creator>Song, Zhangqing</creator><creator>Xu, Xianhao</creator><creator>Ma, Zheng</creator><creator>Bechthold, Andreas</creator><creator>Yu, Xiaoping</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20190901</creationdate><title>ToyA, a positive pathway-specific regulator for toyocamycin biosynthesis in Streptomyces diastatochromogenes 1628</title><author>Xu, Jie ; Song, Zhangqing ; Xu, Xianhao ; Ma, Zheng ; Bechthold, Andreas ; Yu, Xiaoping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-db6cb46da165067995b071932b90e8c009bedc66cdca7a95126d82ddf51b764e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibiotics</topic><topic>Applied Genetics and Molecular Biotechnology</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways - genetics</topic><topic>Biotechnology</topic><topic>Complementation</topic><topic>Disruption</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Fungi</topic><topic>Gene deletion</topic><topic>Gene disruption</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Genetic transcription</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>Overexpression</topic><topic>Physiological aspects</topic><topic>Polymerase chain reaction</topic><topic>Promoter Regions, Genetic</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces diastatochromogenes</topic><topic>Toyocamycin - biosynthesis</topic><topic>Transcription</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Jie</creatorcontrib><creatorcontrib>Song, Zhangqing</creatorcontrib><creatorcontrib>Xu, Xianhao</creatorcontrib><creatorcontrib>Ma, Zheng</creatorcontrib><creatorcontrib>Bechthold, Andreas</creatorcontrib><creatorcontrib>Yu, Xiaoping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Jie</au><au>Song, Zhangqing</au><au>Xu, Xianhao</au><au>Ma, Zheng</au><au>Bechthold, Andreas</au><au>Yu, Xiaoping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ToyA, a positive pathway-specific regulator for toyocamycin biosynthesis in Streptomyces diastatochromogenes 1628</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>103</volume><issue>17</issue><spage>7071</spage><epage>7084</epage><pages>7071-7084</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>The nucleoside antibiotic toyocamycin (TM), which was produced by
Streptomyces diastatochromogenes
1628, was found to be highly efficient against a broad range of plant pathogenic fungi. Despite its importance, little is known about the regulation TM biosynthesis. In this study,
toyA
, located in the TM biosynthetic gene cluster, was identified as a regulatory gene encoding a large ATP-binding regulator of the LuxR family (LAL-family). The role of
toyA
in TM biosynthesis in
S. diastatochromogenes
1628 was investigated by gene deletion, complementation, and over-expression. Gene disruption of
toyA
resulted in almost loss of TM production. TM production in complemented strain was restored to the level comparable to that in the wild-type strain
S. diastatochromogenes
1628. Over-expression of
toyA
separately controlled by promoter SPL57, SPL21, and p
erm
E
*
in wild-type strain
S. diastatochromogenes
1628 led to a 2-fold, 1-fold, and 80% increase in TM production compared with wild-type strain
S. diastatochromogenes
1628, respectively. Quantitative RT-PCR analysis revealed that the transcriptional level of
toy
structural genes was downregulated in the ΔtoyA mutant but restored in complemented strain and further upregulated in the
toyA
over-expression strain. The detection results from GFP reporter system in
Escherichia coli
and GUS reporter system and GUS activities in
S. albus
J1074 and
S. diastatochromogenes
1628 showed that ToyA activated the expression of
toyB
and
toyE
operon directly and activated the expression of other
toy
structural genes indirectly. These results indicate that ToyA is essential for TM biosynthesis controlling the expression of structural genes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31256228</pmid><doi>10.1007/s00253-019-09959-w</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2019-09, Vol.103 (17), p.7071-7084 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2250621508 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Antibiotics Applied Genetics and Molecular Biotechnology Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Biosynthesis Biosynthetic Pathways - genetics Biotechnology Complementation Disruption E coli Escherichia coli Fungi Gene deletion Gene disruption Gene Expression Gene Expression Regulation, Bacterial Genes Genetic research Genetic transcription Life Sciences Microbial Genetics and Genomics Microbiology Multigene Family Mutation Overexpression Physiological aspects Polymerase chain reaction Promoter Regions, Genetic Streptomyces - genetics Streptomyces - metabolism Streptomyces diastatochromogenes Toyocamycin - biosynthesis Transcription Transcription Factors - genetics Transcription Factors - metabolism |
| title | ToyA, a positive pathway-specific regulator for toyocamycin biosynthesis in Streptomyces diastatochromogenes 1628 |
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