Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress
The unicellular green microalga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin under stress conditions such as nitrogen deficiency. In this study, we discovered an astaxanthin accumulation in H. pluvialis cells by the addition of a synthetic cationic polymer,...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2019-12, Vol.128 (6), p.751-754 |
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| container_end_page | 754 |
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| container_issue | 6 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Yoshitomi, Toru Shimada, Naoya Iijima, Kazutoshi Hashizume, Mineo Yoshimoto, Keitaro |
| description | The unicellular green microalga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin under stress conditions such as nitrogen deficiency. In this study, we discovered an astaxanthin accumulation in H. pluvialis cells by the addition of a synthetic cationic polymer, polyethyleneimine (PEI), into the cell culture. With an increase in PEI amount, amount of astaxanthin accumulation was increased. To investigate the mechanism for the accumulation of astaxanthin by the addition of PEI in H. pluvialis cells, we measured a localization of PEI in the cells and a production of reactive oxygen species. PEI was internalized in the cells through the negatively-charged cell walls, leading to excessive production of reactive oxygen species in the cells. Thus, the increased oxidative stress by cellular uptake of PEI resulted in the acceleration of astaxanthin accumulation in H. pluvialis.
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| doi_str_mv | 10.1016/j.jbiosc.2019.06.002 |
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[Display omitted]</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2019.06.002</identifier><identifier>PMID: 31253510</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>Astaxanthin ; Cellular uptake ; Chlorophyta - drug effects ; Chlorophyta - metabolism ; Color ; Flocculant ; Haematococcus pluvialis ; Microalgae ; Oxidative stress ; Oxidative Stress - drug effects ; Polyethyleneimine ; Polyethyleneimine - chemistry ; Polyethyleneimine - pharmacology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Xanthophylls - metabolism</subject><ispartof>Journal of bioscience and bioengineering, 2019-12, Vol.128 (6), p.751-754</ispartof><rights>2019 The Society for Biotechnology, Japan</rights><rights>Copyright © 2019 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-2e563fa078ce10fc5c39a02b815d28627cf590928efd5ea883dedba2fdaca53b3</citedby><cites>FETCH-LOGICAL-c492t-2e563fa078ce10fc5c39a02b815d28627cf590928efd5ea883dedba2fdaca53b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiosc.2019.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31253510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshitomi, Toru</creatorcontrib><creatorcontrib>Shimada, Naoya</creatorcontrib><creatorcontrib>Iijima, Kazutoshi</creatorcontrib><creatorcontrib>Hashizume, Mineo</creatorcontrib><creatorcontrib>Yoshimoto, Keitaro</creatorcontrib><title>Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>The unicellular green microalga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin under stress conditions such as nitrogen deficiency. In this study, we discovered an astaxanthin accumulation in H. pluvialis cells by the addition of a synthetic cationic polymer, polyethyleneimine (PEI), into the cell culture. With an increase in PEI amount, amount of astaxanthin accumulation was increased. To investigate the mechanism for the accumulation of astaxanthin by the addition of PEI in H. pluvialis cells, we measured a localization of PEI in the cells and a production of reactive oxygen species. PEI was internalized in the cells through the negatively-charged cell walls, leading to excessive production of reactive oxygen species in the cells. Thus, the increased oxidative stress by cellular uptake of PEI resulted in the acceleration of astaxanthin accumulation in H. pluvialis.
[Display omitted]</description><subject>Astaxanthin</subject><subject>Cellular uptake</subject><subject>Chlorophyta - drug effects</subject><subject>Chlorophyta - metabolism</subject><subject>Color</subject><subject>Flocculant</subject><subject>Haematococcus pluvialis</subject><subject>Microalgae</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Polyethyleneimine</subject><subject>Polyethyleneimine - chemistry</subject><subject>Polyethyleneimine - pharmacology</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Xanthophylls - metabolism</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P3DAQhi1EBRT4B6jKkUtSf8T5uCBViBYkpHIoZ2tiT1ivnHhrOyuWX1-vFnrk5LHmmXc0DyFXjFaMsub7uloP1kddccr6ijYVpfyInDFRt2Vdc3a8r7u-ZC0Xp-RrjGtKWUtbdkJOBeNSSEbPyNuTdztMq53DGe1kZyztbBaNpoCY4BXmtLJzAVov0-IgWT8X-Z9WWLwExNxxL1DcA06QvPYZi8XGLVsLzsZi2GVYB4SY8_yrNTlgi0VMAWO8IF9GcBEv399z8vzz7s_tffn4-9fD7Y_HUtc9TyVH2YgRaNtpZHTUUoseKB86Jg3vGt7qUfa05x2ORiJ0nTBoBuCjAQ1SDOKcXB9yN8H_XTAmNdmo0TmY0S9RcS5pwwSr24zWB1QHH2PAUW2CnSDsFKNqb12t1cG62ltXtFHZeh779r5hGSY0_4c-NGfg5gBgvnNrMaioLc7Zsg2okzLefr7hH0EkmS4</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Yoshitomi, Toru</creator><creator>Shimada, Naoya</creator><creator>Iijima, Kazutoshi</creator><creator>Hashizume, Mineo</creator><creator>Yoshimoto, Keitaro</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20191201</creationdate><title>Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress</title><author>Yoshitomi, Toru ; Shimada, Naoya ; Iijima, Kazutoshi ; Hashizume, Mineo ; Yoshimoto, Keitaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-2e563fa078ce10fc5c39a02b815d28627cf590928efd5ea883dedba2fdaca53b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Astaxanthin</topic><topic>Cellular uptake</topic><topic>Chlorophyta - drug effects</topic><topic>Chlorophyta - metabolism</topic><topic>Color</topic><topic>Flocculant</topic><topic>Haematococcus pluvialis</topic><topic>Microalgae</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Polyethyleneimine</topic><topic>Polyethyleneimine - chemistry</topic><topic>Polyethyleneimine - pharmacology</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Xanthophylls - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshitomi, Toru</creatorcontrib><creatorcontrib>Shimada, Naoya</creatorcontrib><creatorcontrib>Iijima, Kazutoshi</creatorcontrib><creatorcontrib>Hashizume, Mineo</creatorcontrib><creatorcontrib>Yoshimoto, Keitaro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshitomi, Toru</au><au>Shimada, Naoya</au><au>Iijima, Kazutoshi</au><au>Hashizume, Mineo</au><au>Yoshimoto, Keitaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>128</volume><issue>6</issue><spage>751</spage><epage>754</epage><pages>751-754</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>The unicellular green microalga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin under stress conditions such as nitrogen deficiency. In this study, we discovered an astaxanthin accumulation in H. pluvialis cells by the addition of a synthetic cationic polymer, polyethyleneimine (PEI), into the cell culture. With an increase in PEI amount, amount of astaxanthin accumulation was increased. To investigate the mechanism for the accumulation of astaxanthin by the addition of PEI in H. pluvialis cells, we measured a localization of PEI in the cells and a production of reactive oxygen species. PEI was internalized in the cells through the negatively-charged cell walls, leading to excessive production of reactive oxygen species in the cells. Thus, the increased oxidative stress by cellular uptake of PEI resulted in the acceleration of astaxanthin accumulation in H. pluvialis.
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| ispartof | Journal of bioscience and bioengineering, 2019-12, Vol.128 (6), p.751-754 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Astaxanthin Cellular uptake Chlorophyta - drug effects Chlorophyta - metabolism Color Flocculant Haematococcus pluvialis Microalgae Oxidative stress Oxidative Stress - drug effects Polyethyleneimine Polyethyleneimine - chemistry Polyethyleneimine - pharmacology Reactive oxygen species Reactive Oxygen Species - metabolism Xanthophylls - metabolism |
| title | Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress |
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