Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment
To compare effects of platelet-rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP), autologous processed plasma (APP), and sodium hyaluronate treatments on synovial fluid cells in vitro and on synovial fluid obtained from osteochondrotic joints of horses. Synovial fluid cells from 8...
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| Veröffentlicht in: | American journal of veterinary research 2019-07, Vol.80 (7), p.646-656 |
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| creator | Machado, Thaís S L Massoco, Cristina O Silva, Luis Cláudio L C Fülber, Joice Moreira, Juliana J Baccarin, Raquel Y A |
| description | To compare effects of platelet-rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP), autologous processed plasma (APP), and sodium hyaluronate treatments on synovial fluid cells in vitro and on synovial fluid obtained from osteochondrotic joints of horses.
Synovial fluid cells from 8 healthy equine tibiotarsal joints (in vitro experiment) and synovial fluid samples from 40 tibiotarsal joints of 25 horses with osteochondrosis dissecans (in vivo experiment).
Effects of various treatments on concentrations of prostaglandin (PG) E
, interleukin (IL)-1β, tumor necrosis factor-α, IL-10, and IL-1 receptor antagonist (IL-1ra) were analyzed in cell medium supernatant, and production of reactive oxygen species was analyzed by use of flow cytometry. In an in vivo experiment, synovial fluid samples were collected before and 48 hours after arthroscopy and treatment administration (8 joints/treatment) and evaluated to determine concentrations of hyaluronic acid, chondroitin sulfate, PGE
, tumor necrosis factor-α, IL-1, IL-10, and IL-1ra.
All in vitro treatments reduced reactive oxygen species production, PRP increased PGE
concentrations, and PRP, IRAP, and APP increased IL-1ra concentrations. Only IRAP and APP increased IL-1 concentrations. For the in vivo experiment, PRP increased and IRAP decreased PGE
concentrations in synovial fluid after arthroscopy. All treatments increased IL-1ra concentrations, but only sodium hyaluronate resulted in a significant increase in concentration, compared with the concentration for untreated joints. Also, IRAP reduced hyaluronic acid breakdown in synovial fluid.
PRP should be used with caution in the period immediately after arthroscopy and treatment of osteochondrotic joints of horses. All treatments had antioxidant effects. Sodium hyaluronate, APP, and IRAP might help ameliorate joint inflammation. |
| doi_str_mv | 10.2460/ajvr.80.7.646 |
| format | Article |
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Synovial fluid cells from 8 healthy equine tibiotarsal joints (in vitro experiment) and synovial fluid samples from 40 tibiotarsal joints of 25 horses with osteochondrosis dissecans (in vivo experiment).
Effects of various treatments on concentrations of prostaglandin (PG) E
, interleukin (IL)-1β, tumor necrosis factor-α, IL-10, and IL-1 receptor antagonist (IL-1ra) were analyzed in cell medium supernatant, and production of reactive oxygen species was analyzed by use of flow cytometry. In an in vivo experiment, synovial fluid samples were collected before and 48 hours after arthroscopy and treatment administration (8 joints/treatment) and evaluated to determine concentrations of hyaluronic acid, chondroitin sulfate, PGE
, tumor necrosis factor-α, IL-1, IL-10, and IL-1ra.
All in vitro treatments reduced reactive oxygen species production, PRP increased PGE
concentrations, and PRP, IRAP, and APP increased IL-1ra concentrations. Only IRAP and APP increased IL-1 concentrations. For the in vivo experiment, PRP increased and IRAP decreased PGE
concentrations in synovial fluid after arthroscopy. All treatments increased IL-1ra concentrations, but only sodium hyaluronate resulted in a significant increase in concentration, compared with the concentration for untreated joints. Also, IRAP reduced hyaluronic acid breakdown in synovial fluid.
PRP should be used with caution in the period immediately after arthroscopy and treatment of osteochondrotic joints of horses. All treatments had antioxidant effects. Sodium hyaluronate, APP, and IRAP might help ameliorate joint inflammation.</description><identifier>ISSN: 0002-9645</identifier><identifier>EISSN: 1943-5681</identifier><identifier>DOI: 10.2460/ajvr.80.7.646</identifier><identifier>PMID: 31246124</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Arthroscopy - veterinary ; Female ; Horses ; Hyaluronic Acid - administration & dosage ; Injections, Intra-Articular - veterinary ; Interleukin 1 Receptor Antagonist Protein - administration & dosage ; Joints - drug effects ; Joints - physiopathology ; Male ; Plasma - chemistry ; Platelet-Rich Plasma - chemistry ; Synovial Fluid - drug effects ; Synovial Fluid - metabolism</subject><ispartof>American journal of veterinary research, 2019-07, Vol.80 (7), p.646-656</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c293t-8c7ac623be9e9c2656d9d92648b883ec325f71b54ec530f7b0097436ec0fd7553</citedby><cites>FETCH-LOGICAL-c293t-8c7ac623be9e9c2656d9d92648b883ec325f71b54ec530f7b0097436ec0fd7553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31246124$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Machado, Thaís S L</creatorcontrib><creatorcontrib>Massoco, Cristina O</creatorcontrib><creatorcontrib>Silva, Luis Cláudio L C</creatorcontrib><creatorcontrib>Fülber, Joice</creatorcontrib><creatorcontrib>Moreira, Juliana J</creatorcontrib><creatorcontrib>Baccarin, Raquel Y A</creatorcontrib><title>Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment</title><title>American journal of veterinary research</title><addtitle>Am J Vet Res</addtitle><description>To compare effects of platelet-rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP), autologous processed plasma (APP), and sodium hyaluronate treatments on synovial fluid cells in vitro and on synovial fluid obtained from osteochondrotic joints of horses.
Synovial fluid cells from 8 healthy equine tibiotarsal joints (in vitro experiment) and synovial fluid samples from 40 tibiotarsal joints of 25 horses with osteochondrosis dissecans (in vivo experiment).
Effects of various treatments on concentrations of prostaglandin (PG) E
, interleukin (IL)-1β, tumor necrosis factor-α, IL-10, and IL-1 receptor antagonist (IL-1ra) were analyzed in cell medium supernatant, and production of reactive oxygen species was analyzed by use of flow cytometry. In an in vivo experiment, synovial fluid samples were collected before and 48 hours after arthroscopy and treatment administration (8 joints/treatment) and evaluated to determine concentrations of hyaluronic acid, chondroitin sulfate, PGE
, tumor necrosis factor-α, IL-1, IL-10, and IL-1ra.
All in vitro treatments reduced reactive oxygen species production, PRP increased PGE
concentrations, and PRP, IRAP, and APP increased IL-1ra concentrations. Only IRAP and APP increased IL-1 concentrations. For the in vivo experiment, PRP increased and IRAP decreased PGE
concentrations in synovial fluid after arthroscopy. All treatments increased IL-1ra concentrations, but only sodium hyaluronate resulted in a significant increase in concentration, compared with the concentration for untreated joints. Also, IRAP reduced hyaluronic acid breakdown in synovial fluid.
PRP should be used with caution in the period immediately after arthroscopy and treatment of osteochondrotic joints of horses. All treatments had antioxidant effects. Sodium hyaluronate, APP, and IRAP might help ameliorate joint inflammation.</description><subject>Animals</subject><subject>Arthroscopy - veterinary</subject><subject>Female</subject><subject>Horses</subject><subject>Hyaluronic Acid - administration & dosage</subject><subject>Injections, Intra-Articular - veterinary</subject><subject>Interleukin 1 Receptor Antagonist Protein - administration & dosage</subject><subject>Joints - drug effects</subject><subject>Joints - physiopathology</subject><subject>Male</subject><subject>Plasma - chemistry</subject><subject>Platelet-Rich Plasma - chemistry</subject><subject>Synovial Fluid - drug effects</subject><subject>Synovial Fluid - metabolism</subject><issn>0002-9645</issn><issn>1943-5681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkT9vFDEQxS1ElBxJSlrkkmYPr_-tt0RRAkiRaKC2vPZY59OufbG9J90H5Hvh4wIFxWiK-b2nmXkIve_JlnJJPpn9MW8V2Q5byeUbtOlHzjohVf8WbQghtBslFzfoXSl7QnqqenGNbljftK026Nej92BrwcnjaU7JdQ5yOILDh5zcep6Y6HBJLqwL3p3MvOYUTQWcIoaXNUTA5RTTMZgZ-3kNDluY54uqIf_NfE4LTqVCsrsUXU41WLxPIV422KVcoGl9hYxNrrucik2H0x8345YQQ6nZ1NCcG14zmLpArHfoypu5wP1rv0U_nx5_PHztnr9_-fbw-bmzdGS1U3YwVlI2wQijpVJIN7qRSq4mpRhYRoUf-klwsIIRP0yEjANnEizxbhCC3aKPF9_2nJcVStVLKOd7TYS0Fk0pV0xxLoeGdhfUthtKBq8POSwmn3RP9Dk5fU5OK6IH3ZJr_IdX63VawP2j_0bFfgNz9psy</recordid><startdate>201907</startdate><enddate>201907</enddate><creator>Machado, Thaís S L</creator><creator>Massoco, Cristina O</creator><creator>Silva, Luis Cláudio L C</creator><creator>Fülber, Joice</creator><creator>Moreira, Juliana J</creator><creator>Baccarin, Raquel Y A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201907</creationdate><title>Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment</title><author>Machado, Thaís S L ; Massoco, Cristina O ; Silva, Luis Cláudio L C ; Fülber, Joice ; Moreira, Juliana J ; Baccarin, Raquel Y A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c293t-8c7ac623be9e9c2656d9d92648b883ec325f71b54ec530f7b0097436ec0fd7553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Arthroscopy - veterinary</topic><topic>Female</topic><topic>Horses</topic><topic>Hyaluronic Acid - administration & dosage</topic><topic>Injections, Intra-Articular - veterinary</topic><topic>Interleukin 1 Receptor Antagonist Protein - administration & dosage</topic><topic>Joints - drug effects</topic><topic>Joints - physiopathology</topic><topic>Male</topic><topic>Plasma - chemistry</topic><topic>Platelet-Rich Plasma - chemistry</topic><topic>Synovial Fluid - drug effects</topic><topic>Synovial Fluid - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Machado, Thaís S L</creatorcontrib><creatorcontrib>Massoco, Cristina O</creatorcontrib><creatorcontrib>Silva, Luis Cláudio L C</creatorcontrib><creatorcontrib>Fülber, Joice</creatorcontrib><creatorcontrib>Moreira, Juliana J</creatorcontrib><creatorcontrib>Baccarin, Raquel Y A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Machado, Thaís S L</au><au>Massoco, Cristina O</au><au>Silva, Luis Cláudio L C</au><au>Fülber, Joice</au><au>Moreira, Juliana J</au><au>Baccarin, Raquel Y A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment</atitle><jtitle>American journal of veterinary research</jtitle><addtitle>Am J Vet Res</addtitle><date>2019-07</date><risdate>2019</risdate><volume>80</volume><issue>7</issue><spage>646</spage><epage>656</epage><pages>646-656</pages><issn>0002-9645</issn><eissn>1943-5681</eissn><abstract>To compare effects of platelet-rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP), autologous processed plasma (APP), and sodium hyaluronate treatments on synovial fluid cells in vitro and on synovial fluid obtained from osteochondrotic joints of horses.
Synovial fluid cells from 8 healthy equine tibiotarsal joints (in vitro experiment) and synovial fluid samples from 40 tibiotarsal joints of 25 horses with osteochondrosis dissecans (in vivo experiment).
Effects of various treatments on concentrations of prostaglandin (PG) E
, interleukin (IL)-1β, tumor necrosis factor-α, IL-10, and IL-1 receptor antagonist (IL-1ra) were analyzed in cell medium supernatant, and production of reactive oxygen species was analyzed by use of flow cytometry. In an in vivo experiment, synovial fluid samples were collected before and 48 hours after arthroscopy and treatment administration (8 joints/treatment) and evaluated to determine concentrations of hyaluronic acid, chondroitin sulfate, PGE
, tumor necrosis factor-α, IL-1, IL-10, and IL-1ra.
All in vitro treatments reduced reactive oxygen species production, PRP increased PGE
concentrations, and PRP, IRAP, and APP increased IL-1ra concentrations. Only IRAP and APP increased IL-1 concentrations. For the in vivo experiment, PRP increased and IRAP decreased PGE
concentrations in synovial fluid after arthroscopy. All treatments increased IL-1ra concentrations, but only sodium hyaluronate resulted in a significant increase in concentration, compared with the concentration for untreated joints. Also, IRAP reduced hyaluronic acid breakdown in synovial fluid.
PRP should be used with caution in the period immediately after arthroscopy and treatment of osteochondrotic joints of horses. All treatments had antioxidant effects. Sodium hyaluronate, APP, and IRAP might help ameliorate joint inflammation.</abstract><cop>United States</cop><pmid>31246124</pmid><doi>10.2460/ajvr.80.7.646</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Arthroscopy - veterinary Female Horses Hyaluronic Acid - administration & dosage Injections, Intra-Articular - veterinary Interleukin 1 Receptor Antagonist Protein - administration & dosage Joints - drug effects Joints - physiopathology Male Plasma - chemistry Platelet-Rich Plasma - chemistry Synovial Fluid - drug effects Synovial Fluid - metabolism |
| title | Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment |
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