Multicenter Evaluation of Circulating Plasma MicroRNA Extraction Technologies for the Development of Clinically Feasible Reverse Transcription Quantitative PCR and Next-Generation Sequencing Analytical Work Flows

In human body fluids, microRNA (miRNA) can be found as circulating cell-free miRNA (cfmiRNA), as well as secreted into extracellular vesicles (EVmiRNA). miRNAs are being intensively evaluated as minimally invasive liquid biopsy biomarkers in patients with cancer. The growing interest in developing c...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2019-09, Vol.65 (9), p.1132-1140
Hauptverfasser:	Kloten, Vera, Neumann, Martin H D, Di Pasquale, Francesca, Sprenger-Haussels, Markus, Shaffer, Jonathan M, Schlumpberger, Martin, Herdean, Andrei, Betsou, Fay, Ammerlaan, Wim, Af Hällström, Taija, Serkkola, Elina, Forsman, Tarja, Lianidou, Evi, Sjöback, Robert, Kubista, Mikael, Bender, Sebastian, Lampignano, Rita, Krahn, Thomas, Schlange, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Animals Biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - isolation & purification Biopsy Blood & organ donations Body fluids Breast cancer Caenorhabditis elegans - chemistry Chemical Fractionation - methods Circulating MicroRNA - blood Circulating MicroRNA - isolation & purification Cluster analysis Extracellular Vesicles - chemistry Female Gene expression High-Throughput Nucleotide Sequencing - methods Humans Kinases Lung cancer Male Measurement methods Medical research MicroRNAs Middle Aged miRNA Next-generation sequencing Phenols Plasma Purification Real-Time Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription Ribonucleic acid RNA Studies Ultracentrifugation
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