LncRNA GAS5 regulates the proliferation, migration, invasion and apoptosis of brain glioma cells through targeting GSTM3 expression. The effect of LncRNA GAS5 on glioma cells

Introduction To investigate the effects of lncRNA GAS5 on the proliferation, migration, invasion and apoptosis of brain glioma cells. Methods The expression levels of lncRNA GAS5 and GSTM3 in normal glial cells (HEB) and glioma cells (U251 and U87) were detected by RT-qPCR and western blot, respecti...

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Veröffentlicht in:	Journal of neuro-oncology 2019-07, Vol.143 (3), p.525-536
Hauptverfasser:	Li, Guoxiong, Cai, Yingqian, Wang, Chuanmei, Huang, Min, Chen, Jiansheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Bioinformatics Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Case-Control Studies Caspase Caspase-3 Cell lines Cell Movement Cell Proliferation Cholecystokinin Clinical Study Female Follow-Up Studies Gene Expression Regulation, Neoplastic Glial cells Glioma Glioma - genetics Glioma - metabolism Glioma - pathology Glioma cells Glutathione Transferase - genetics Glutathione Transferase - metabolism Humans Medicine Medicine & Public Health Mice Mice, Nude Neoplasm Invasiveness Neurology Oncology Overexpression Prognosis RNA, Long Noncoding - genetics siRNA Tumor Cells, Cultured Western blotting Xenograft Model Antitumor Assays
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Zusammenfassung:	Introduction To investigate the effects of lncRNA GAS5 on the proliferation, migration, invasion and apoptosis of brain glioma cells. Methods The expression levels of lncRNA GAS5 and GSTM3 in normal glial cells (HEB) and glioma cells (U251 and U87) were detected by RT-qPCR and western blot, respectively. Glioma cells were transfected with ctrl vector, pcDNA-GAS5, siRNA ctrl (siNC) or GSTM3 siRNA and the effects of lncRNA GAS5 and GSTM3 on the proliferation, migration, invasion and apoptosis of glioma cells were detected by CCK-8 assay, transwell assay and Caspase 3/7 activity assay, respectively. Results The expression of lncRNA GAS5 was significantly decreased in glioma cell lines U251 and U87 compared with normal glial cells HEB (p
ISSN:	0167-594X 1573-7373
DOI:	10.1007/s11060-019-03185-0