In vivo hepatoprotective and In vitro radical scavenging activities of Cucumis ficifolius A. rich root extract
Cucumis ficifolius A. Rich is a perennial prostrate herb that stems up to 1 m long. Its root is widely used in traditional medicine to treat various diseases including liver diseases. Yet, scientific evidence is lacking to verify its ethno medicinal claims. Aim of the study: The present study was co...
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| Veröffentlicht in: | Journal of ethnopharmacology 2019-10, Vol.242, p.112031-112031, Article 112031 |
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| container_title | Journal of ethnopharmacology |
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| creator | Araya, Ephrem Mebrahtu Adamu, Betelhem Anteneh Periasamy, Gomathi Sintayehu, Biruk Gebrelibanos Hiben, Mebrahtom |
| description | Cucumis ficifolius A. Rich is a perennial prostrate herb that stems up to 1 m long. Its root is widely used in traditional medicine to treat various diseases including liver diseases. Yet, scientific evidence is lacking to verify its ethno medicinal claims. Aim of the study: The present study was conducted to assess the hepatoprotective and radical scavenging activity of 80% methanol crude extract and different fractions of Cucumis ficifolius root.
Radical scavenging activity was done applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay while hepatoprotective activity was assessed using pre- and post-treatment models of carbon tetrachloride (CCl4)-induced liver injury in Swiss albino mice of either sex weighing 25–30 g. A single oral dose of 2000 mg/kg was used for acute toxicity study, doses of 125, 250, and 500 mg/kg were used in the pre-treatment model, and 500 mg/kg of extract and chloroform fraction were used in the post-treatment model. Biochemical markers and histopathological parameters were used to measure hepatoprotective activities.
C. ficifolius crude extract and its solvent fractions showed strong radical scavenging activity and the chloroform fraction had the highest activity. No sign of toxicity was shown in an acute toxicity test of the extract. Hepatoprotective activity evaluation on the crude extract by a pre-treatment model with 125, 250, and 500 mg/kg doses revealed a significant (p |
| doi_str_mv | 10.1016/j.jep.2019.112031 |
| format | Article |
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Radical scavenging activity was done applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay while hepatoprotective activity was assessed using pre- and post-treatment models of carbon tetrachloride (CCl4)-induced liver injury in Swiss albino mice of either sex weighing 25–30 g. A single oral dose of 2000 mg/kg was used for acute toxicity study, doses of 125, 250, and 500 mg/kg were used in the pre-treatment model, and 500 mg/kg of extract and chloroform fraction were used in the post-treatment model. Biochemical markers and histopathological parameters were used to measure hepatoprotective activities.
C. ficifolius crude extract and its solvent fractions showed strong radical scavenging activity and the chloroform fraction had the highest activity. No sign of toxicity was shown in an acute toxicity test of the extract. Hepatoprotective activity evaluation on the crude extract by a pre-treatment model with 125, 250, and 500 mg/kg doses revealed a significant (p < 0.05) reduction of the serum level of CCl4-induced liver enzyme markers at the highest tested dose (500 mg/kg). The chloroform fraction that had highest radical scavenging activity and the crude extract, both at 500 mg/kg, were again evaluated in a post-treatment model and the results revealed that both the extract and the chloroform fraction demonstrated significant (p < 0.05) hepatoprotective activities which support the results of the pre-treatment model.
The present study verified the hepatoprotective potentials of C. ficifolius extract and its chloroform fraction which might be, at least in part, through radical scavenging action.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2019.112031</identifier><identifier>PMID: 31220599</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; Biphenyl Compounds - chemistry ; Carbon Tetrachloride ; Chemical and Drug Induced Liver Injury - drug therapy ; Chemical and Drug Induced Liver Injury - pathology ; Cucumis ; Cucumis ficifolius ; Female ; Hepatoprotective ; Liver - drug effects ; Liver - pathology ; Male ; Mice ; Picrates - chemistry ; Plant Extracts - chemistry ; Plant Extracts - therapeutic use ; Plant Extracts - toxicity ; Plant Roots ; Protective Agents - chemistry ; Protective Agents - therapeutic use ; Protective Agents - toxicity ; Radical scavenging ; Toxicity Tests, Acute</subject><ispartof>Journal of ethnopharmacology, 2019-10, Vol.242, p.112031-112031, Article 112031</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-677291cf0df92ef25e844962bbb597c4f9c3db1db244e3ba9450f4243f0600c63</citedby><cites>FETCH-LOGICAL-c353t-677291cf0df92ef25e844962bbb597c4f9c3db1db244e3ba9450f4243f0600c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2019.112031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31220599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araya, Ephrem Mebrahtu</creatorcontrib><creatorcontrib>Adamu, Betelhem Anteneh</creatorcontrib><creatorcontrib>Periasamy, Gomathi</creatorcontrib><creatorcontrib>Sintayehu, Biruk</creatorcontrib><creatorcontrib>Gebrelibanos Hiben, Mebrahtom</creatorcontrib><title>In vivo hepatoprotective and In vitro radical scavenging activities of Cucumis ficifolius A. rich root extract</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Cucumis ficifolius A. Rich is a perennial prostrate herb that stems up to 1 m long. Its root is widely used in traditional medicine to treat various diseases including liver diseases. Yet, scientific evidence is lacking to verify its ethno medicinal claims. Aim of the study: The present study was conducted to assess the hepatoprotective and radical scavenging activity of 80% methanol crude extract and different fractions of Cucumis ficifolius root.
Radical scavenging activity was done applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay while hepatoprotective activity was assessed using pre- and post-treatment models of carbon tetrachloride (CCl4)-induced liver injury in Swiss albino mice of either sex weighing 25–30 g. A single oral dose of 2000 mg/kg was used for acute toxicity study, doses of 125, 250, and 500 mg/kg were used in the pre-treatment model, and 500 mg/kg of extract and chloroform fraction were used in the post-treatment model. Biochemical markers and histopathological parameters were used to measure hepatoprotective activities.
C. ficifolius crude extract and its solvent fractions showed strong radical scavenging activity and the chloroform fraction had the highest activity. No sign of toxicity was shown in an acute toxicity test of the extract. Hepatoprotective activity evaluation on the crude extract by a pre-treatment model with 125, 250, and 500 mg/kg doses revealed a significant (p < 0.05) reduction of the serum level of CCl4-induced liver enzyme markers at the highest tested dose (500 mg/kg). The chloroform fraction that had highest radical scavenging activity and the crude extract, both at 500 mg/kg, were again evaluated in a post-treatment model and the results revealed that both the extract and the chloroform fraction demonstrated significant (p < 0.05) hepatoprotective activities which support the results of the pre-treatment model.
The present study verified the hepatoprotective potentials of C. ficifolius extract and its chloroform fraction which might be, at least in part, through radical scavenging action.
[Display omitted]</description><subject>Animals</subject><subject>Biphenyl Compounds - chemistry</subject><subject>Carbon Tetrachloride</subject><subject>Chemical and Drug Induced Liver Injury - drug therapy</subject><subject>Chemical and Drug Induced Liver Injury - pathology</subject><subject>Cucumis</subject><subject>Cucumis ficifolius</subject><subject>Female</subject><subject>Hepatoprotective</subject><subject>Liver - drug effects</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Picrates - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - therapeutic use</subject><subject>Plant Extracts - toxicity</subject><subject>Plant Roots</subject><subject>Protective Agents - chemistry</subject><subject>Protective Agents - therapeutic use</subject><subject>Protective Agents - toxicity</subject><subject>Radical scavenging</subject><subject>Toxicity Tests, Acute</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1P5DAQhq0T6Fg-fgANckmT4K_Ea1GhFXeHtNI1UFuOMwavsvFiOxH8-_OyHCXVFPO8r2YehC4pqSmh7c2m3sCuZoSqmlJGOP2BFnQpWSUbyY_QgnC5rJZS0BN0mtKGECKpID_RCaeMkUapBRofRjz7OeAX2JkcdjFksNnPgM3Y449ljgFH03trBpysmWF89uMzNnvMZw8JB4dXk522PmHnrXdh8FPCdzWO3r7gGELG8JZjSZyjY2eGBBef8ww9_bp_XP2p1n9_P6zu1pXlDc9VKyVT1DrSO8XAsQaWQqiWdV3XKGmFU5b3He07JgTwzijRECeY4I60hNiWn6HrQ2956HWClHU5zsIwmBHClDRjomkbXtQVlB5QG0NKEZzeRb818V1Tovea9UYXzXqvWR80l8zVZ_3UbaH_Svz3WoDbAwDlydlD1Ml6GC30Pha_ug_-m_p_WYaOFA</recordid><startdate>20191005</startdate><enddate>20191005</enddate><creator>Araya, Ephrem Mebrahtu</creator><creator>Adamu, Betelhem Anteneh</creator><creator>Periasamy, Gomathi</creator><creator>Sintayehu, Biruk</creator><creator>Gebrelibanos Hiben, Mebrahtom</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20191005</creationdate><title>In vivo hepatoprotective and In vitro radical scavenging activities of Cucumis ficifolius A. rich root extract</title><author>Araya, Ephrem Mebrahtu ; Adamu, Betelhem Anteneh ; Periasamy, Gomathi ; Sintayehu, Biruk ; Gebrelibanos Hiben, Mebrahtom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-677291cf0df92ef25e844962bbb597c4f9c3db1db244e3ba9450f4243f0600c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biphenyl Compounds - chemistry</topic><topic>Carbon Tetrachloride</topic><topic>Chemical and Drug Induced Liver Injury - drug therapy</topic><topic>Chemical and Drug Induced Liver Injury - pathology</topic><topic>Cucumis</topic><topic>Cucumis ficifolius</topic><topic>Female</topic><topic>Hepatoprotective</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Picrates - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - therapeutic use</topic><topic>Plant Extracts - toxicity</topic><topic>Plant Roots</topic><topic>Protective Agents - chemistry</topic><topic>Protective Agents - therapeutic use</topic><topic>Protective Agents - toxicity</topic><topic>Radical scavenging</topic><topic>Toxicity Tests, Acute</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Araya, Ephrem Mebrahtu</creatorcontrib><creatorcontrib>Adamu, Betelhem Anteneh</creatorcontrib><creatorcontrib>Periasamy, Gomathi</creatorcontrib><creatorcontrib>Sintayehu, Biruk</creatorcontrib><creatorcontrib>Gebrelibanos Hiben, Mebrahtom</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Araya, Ephrem Mebrahtu</au><au>Adamu, Betelhem Anteneh</au><au>Periasamy, Gomathi</au><au>Sintayehu, Biruk</au><au>Gebrelibanos Hiben, Mebrahtom</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo hepatoprotective and In vitro radical scavenging activities of Cucumis ficifolius A. rich root extract</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2019-10-05</date><risdate>2019</risdate><volume>242</volume><spage>112031</spage><epage>112031</epage><pages>112031-112031</pages><artnum>112031</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Cucumis ficifolius A. Rich is a perennial prostrate herb that stems up to 1 m long. Its root is widely used in traditional medicine to treat various diseases including liver diseases. Yet, scientific evidence is lacking to verify its ethno medicinal claims. Aim of the study: The present study was conducted to assess the hepatoprotective and radical scavenging activity of 80% methanol crude extract and different fractions of Cucumis ficifolius root.
Radical scavenging activity was done applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay while hepatoprotective activity was assessed using pre- and post-treatment models of carbon tetrachloride (CCl4)-induced liver injury in Swiss albino mice of either sex weighing 25–30 g. A single oral dose of 2000 mg/kg was used for acute toxicity study, doses of 125, 250, and 500 mg/kg were used in the pre-treatment model, and 500 mg/kg of extract and chloroform fraction were used in the post-treatment model. Biochemical markers and histopathological parameters were used to measure hepatoprotective activities.
C. ficifolius crude extract and its solvent fractions showed strong radical scavenging activity and the chloroform fraction had the highest activity. No sign of toxicity was shown in an acute toxicity test of the extract. Hepatoprotective activity evaluation on the crude extract by a pre-treatment model with 125, 250, and 500 mg/kg doses revealed a significant (p < 0.05) reduction of the serum level of CCl4-induced liver enzyme markers at the highest tested dose (500 mg/kg). The chloroform fraction that had highest radical scavenging activity and the crude extract, both at 500 mg/kg, were again evaluated in a post-treatment model and the results revealed that both the extract and the chloroform fraction demonstrated significant (p < 0.05) hepatoprotective activities which support the results of the pre-treatment model.
The present study verified the hepatoprotective potentials of C. ficifolius extract and its chloroform fraction which might be, at least in part, through radical scavenging action.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>31220599</pmid><doi>10.1016/j.jep.2019.112031</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Biphenyl Compounds - chemistry Carbon Tetrachloride Chemical and Drug Induced Liver Injury - drug therapy Chemical and Drug Induced Liver Injury - pathology Cucumis Cucumis ficifolius Female Hepatoprotective Liver - drug effects Liver - pathology Male Mice Picrates - chemistry Plant Extracts - chemistry Plant Extracts - therapeutic use Plant Extracts - toxicity Plant Roots Protective Agents - chemistry Protective Agents - therapeutic use Protective Agents - toxicity Radical scavenging Toxicity Tests, Acute |
| title | In vivo hepatoprotective and In vitro radical scavenging activities of Cucumis ficifolius A. rich root extract |
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