Avoiding pitfalls in diagnostic immunohistochemistry–important technical aspects that every pathologist should know
This review focuses on technical aspects of diagnostic immunohistochemistry (IHC), with an emphasis on aspects of methodology and interpretation that may be problematic for practicing pathologists. Pitfalls in IHC are reviewed, and the importance of good controls and the the use of multi-tissue cont...
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| Veröffentlicht in: | Seminars in diagnostic pathology 2019-09, Vol.36 (5), p.312-335 |
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| container_title | Seminars in diagnostic pathology |
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| creator | Miller, Rodney T. |
| description | This review focuses on technical aspects of diagnostic immunohistochemistry (IHC), with an emphasis on aspects of methodology and interpretation that may be problematic for practicing pathologists. Pitfalls in IHC are reviewed, and the importance of good controls and the the use of multi-tissue controls is discussed. Also covered is the optimal use of IHC in cytologic specimens and specimens where no paraffin block is available. Artifacts encountered in IHC are discussed and illustrated, and a number of useful techniques are described in detail, including preparation of multi-tissue control material, tissue and cell transfer techniques, and tissue protection techniques. |
| doi_str_mv | 10.1053/j.semdp.2019.05.002 |
| format | Article |
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Artifacts encountered in IHC are discussed and illustrated, and a number of useful techniques are described in detail, including preparation of multi-tissue control material, tissue and cell transfer techniques, and tissue protection techniques.</description><identifier>ISSN: 0740-2570</identifier><identifier>EISSN: 1930-1111</identifier><identifier>DOI: 10.1053/j.semdp.2019.05.002</identifier><identifier>PMID: 31227425</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Humans ; Immunohistochemistry ; Immunohistochemistry - methods ; Immunohistochemistry - standards ; Laboratories - standards ; Methodology ; Pathologists ; Pathology, Clinical - methods ; Pathology, Clinical - standards ; Pitfalls ; Quality Control ; Staining and Labeling - methods ; Staining and Labeling - standards ; Tissue Fixation - methods ; Tissue Fixation - standards</subject><ispartof>Seminars in diagnostic pathology, 2019-09, Vol.36 (5), p.312-335</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. 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Artifacts encountered in IHC are discussed and illustrated, and a number of useful techniques are described in detail, including preparation of multi-tissue control material, tissue and cell transfer techniques, and tissue protection techniques.</description><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunohistochemistry - methods</subject><subject>Immunohistochemistry - standards</subject><subject>Laboratories - standards</subject><subject>Methodology</subject><subject>Pathologists</subject><subject>Pathology, Clinical - methods</subject><subject>Pathology, Clinical - standards</subject><subject>Pitfalls</subject><subject>Quality Control</subject><subject>Staining and Labeling - methods</subject><subject>Staining and Labeling - standards</subject><subject>Tissue Fixation - methods</subject><subject>Tissue Fixation - standards</subject><issn>0740-2570</issn><issn>1930-1111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1u2zAUhYmiReP8PEGBgmMXq_wV7SFDEKRNgQBZmpmgqCuLrkSqJOXAW98hb5gnCROnGcPlLt_hwfkQ-kJJRYnk37dVgrGdKkbouiKyIoR9QAu65mRJy_uIFkQJsmRSkSN0nNKWEK4K-xkdccqYEkwu0HyxC651foMnlzszDAk7j1tnNj6k7Cx24zj70LuUg-1hLDfuH_89uHEKMRufcQbbe2fNgE2awOaEc28yhh3EPZ5M7sMQNiWGUx_mocV_fLg_RZ9KV4Kz13uC7n5c_b68Xt7c_vx1eXGztFyuc5khujKhk9Jw25haqXbFOslrRgyzynTWCpBMqBWvgXVCUbviIGraNIyIpuMn6Nvh3ymGvzOkrMsAC8NgPIQ5acaErIWglBWUH1AbQ0oROj1FN5q415ToZ996q19862ffmkhdfJfU19eCuRmhfcv8F1yA8wMAZebOQdTJOvAWWheLLN0G927BEwWjlow</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Miller, Rodney T.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>Avoiding pitfalls in diagnostic immunohistochemistry–important technical aspects that every pathologist should know</title><author>Miller, Rodney T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-114f740f55a3cba677d82f53620a2c7afcc4e5247836e2f471c83e461bb204bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunohistochemistry - methods</topic><topic>Immunohistochemistry - standards</topic><topic>Laboratories - standards</topic><topic>Methodology</topic><topic>Pathologists</topic><topic>Pathology, Clinical - methods</topic><topic>Pathology, Clinical - standards</topic><topic>Pitfalls</topic><topic>Quality Control</topic><topic>Staining and Labeling - methods</topic><topic>Staining and Labeling - standards</topic><topic>Tissue Fixation - methods</topic><topic>Tissue Fixation - standards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, Rodney T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Seminars in diagnostic pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, Rodney T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Avoiding pitfalls in diagnostic immunohistochemistry–important technical aspects that every pathologist should know</atitle><jtitle>Seminars in diagnostic pathology</jtitle><addtitle>Semin Diagn Pathol</addtitle><date>2019-09</date><risdate>2019</risdate><volume>36</volume><issue>5</issue><spage>312</spage><epage>335</epage><pages>312-335</pages><issn>0740-2570</issn><eissn>1930-1111</eissn><abstract>This review focuses on technical aspects of diagnostic immunohistochemistry (IHC), with an emphasis on aspects of methodology and interpretation that may be problematic for practicing pathologists. 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| fulltext | fulltext |
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| ispartof | Seminars in diagnostic pathology, 2019-09, Vol.36 (5), p.312-335 |
| issn | 0740-2570 1930-1111 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Humans Immunohistochemistry Immunohistochemistry - methods Immunohistochemistry - standards Laboratories - standards Methodology Pathologists Pathology, Clinical - methods Pathology, Clinical - standards Pitfalls Quality Control Staining and Labeling - methods Staining and Labeling - standards Tissue Fixation - methods Tissue Fixation - standards |
| title | Avoiding pitfalls in diagnostic immunohistochemistry–important technical aspects that every pathologist should know |
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