Dielectrophoretic deformation of breast cancer cells for lab on a chip applications
This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA‐MB‐231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA‐MB‐231 cel...
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| Veröffentlicht in: | Electrophoresis 2019-10, Vol.40 (20), p.2728-2735 |
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| creator | Chan, Jun Yuan Ahmad Kayani, Aminuddin Bin Md Ali, Mohd Anuar Kok, Chee Kuang Ramdzan Buyong, Muhamad Hoe, Susan Ling Ling Marzuki, Marini Soo‐Beng Khoo, Alan Sriram, Sharath Ostrikov, Kostya (Ken) |
| description | This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA‐MB‐231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA‐MB‐231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA‐MB‐231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field. |
| doi_str_mv | 10.1002/elps.201800442 |
| format | Article |
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The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA‐MB‐231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA‐MB‐231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.201800442</identifier><identifier>PMID: 31219180</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Blebbing ; Breast cancer ; Breast Neoplasms - pathology ; Cancer ; Cell Line, Tumor ; Cell Membrane - physiology ; Cell Shape - physiology ; Cell shrinkage ; Deformation ; Dielectrophoresis ; Electric fields ; Electrophoresis - instrumentation ; Electrophoresis - methods ; Female ; Finite element method ; Humans ; Low conductivity ; Microelectrodes ; Microfluidics ; Shrinkage</subject><ispartof>Electrophoresis, 2019-10, Vol.40 (20), p.2728-2735</ispartof><rights>2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4452-a15271262fbd322e44baf54d1f985f3e4d2b87ada46b03a4930ff09a0ea6c3443</citedby><cites>FETCH-LOGICAL-c4452-a15271262fbd322e44baf54d1f985f3e4d2b87ada46b03a4930ff09a0ea6c3443</cites><orcidid>0000-0003-1841-1716 ; 0000-0003-4772-7613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felps.201800442$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felps.201800442$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31219180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chan, Jun Yuan</creatorcontrib><creatorcontrib>Ahmad Kayani, Aminuddin Bin</creatorcontrib><creatorcontrib>Md Ali, Mohd Anuar</creatorcontrib><creatorcontrib>Kok, Chee Kuang</creatorcontrib><creatorcontrib>Ramdzan Buyong, Muhamad</creatorcontrib><creatorcontrib>Hoe, Susan Ling Ling</creatorcontrib><creatorcontrib>Marzuki, Marini</creatorcontrib><creatorcontrib>Soo‐Beng Khoo, Alan</creatorcontrib><creatorcontrib>Sriram, Sharath</creatorcontrib><creatorcontrib>Ostrikov, Kostya (Ken)</creatorcontrib><title>Dielectrophoretic deformation of breast cancer cells for lab on a chip applications</title><title>Electrophoresis</title><addtitle>Electrophoresis</addtitle><description>This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA‐MB‐231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA‐MB‐231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA‐MB‐231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field.</description><subject>Blebbing</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - physiology</subject><subject>Cell Shape - physiology</subject><subject>Cell shrinkage</subject><subject>Deformation</subject><subject>Dielectrophoresis</subject><subject>Electric fields</subject><subject>Electrophoresis - instrumentation</subject><subject>Electrophoresis - methods</subject><subject>Female</subject><subject>Finite element method</subject><subject>Humans</subject><subject>Low conductivity</subject><subject>Microelectrodes</subject><subject>Microfluidics</subject><subject>Shrinkage</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtLAzEUBeAgitbH1qUE3LiZmtzcmc4sResDCgrVdchkbnBk2ozJFPHfm9rqwo2rQPjO4XIYO5ViLIWAS-r6OAYhSyEQYYeNZA6QQVGqXTYScqIyUar8gB3G-CaSqRD32YGSIKuUGbH5TUsd2SH4_tUHGlrLG3I-LMzQ-iX3jteBTBy4NUtLgVvqusgT4J2peRKG29e256bvu9Z-h-Ix23Omi3SyfY_Yy-30-fo-mz3ePVxfzTKLmENm0qkTCQW4ulEAhFgbl2MjXVXmThE2UJcT0xgsaqEMVko4JyojyBRWIaojdrHp7YN_X1Ec9KKN6wPNkvwqagBEmaNUa3r-h775VVim6zQokSNURQ5JjTfKBh9jIKf70C5M-NRS6PXcej23_p07Bc62tat6Qc0v_9k3AdyAj7ajz3_q9HT2NJ-kf_UF_i6KeQ</recordid><startdate>201910</startdate><enddate>201910</enddate><creator>Chan, Jun Yuan</creator><creator>Ahmad Kayani, Aminuddin Bin</creator><creator>Md Ali, Mohd Anuar</creator><creator>Kok, Chee Kuang</creator><creator>Ramdzan Buyong, Muhamad</creator><creator>Hoe, Susan Ling Ling</creator><creator>Marzuki, Marini</creator><creator>Soo‐Beng Khoo, Alan</creator><creator>Sriram, Sharath</creator><creator>Ostrikov, Kostya (Ken)</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1841-1716</orcidid><orcidid>https://orcid.org/0000-0003-4772-7613</orcidid></search><sort><creationdate>201910</creationdate><title>Dielectrophoretic deformation of breast cancer cells for lab on a chip applications</title><author>Chan, Jun Yuan ; Ahmad Kayani, Aminuddin Bin ; Md Ali, Mohd Anuar ; Kok, Chee Kuang ; Ramdzan Buyong, Muhamad ; Hoe, Susan Ling Ling ; Marzuki, Marini ; Soo‐Beng Khoo, Alan ; Sriram, Sharath ; Ostrikov, Kostya (Ken)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4452-a15271262fbd322e44baf54d1f985f3e4d2b87ada46b03a4930ff09a0ea6c3443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Blebbing</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - physiology</topic><topic>Cell Shape - physiology</topic><topic>Cell shrinkage</topic><topic>Deformation</topic><topic>Dielectrophoresis</topic><topic>Electric fields</topic><topic>Electrophoresis - instrumentation</topic><topic>Electrophoresis - methods</topic><topic>Female</topic><topic>Finite element method</topic><topic>Humans</topic><topic>Low conductivity</topic><topic>Microelectrodes</topic><topic>Microfluidics</topic><topic>Shrinkage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chan, Jun Yuan</creatorcontrib><creatorcontrib>Ahmad Kayani, Aminuddin Bin</creatorcontrib><creatorcontrib>Md Ali, Mohd Anuar</creatorcontrib><creatorcontrib>Kok, Chee Kuang</creatorcontrib><creatorcontrib>Ramdzan Buyong, Muhamad</creatorcontrib><creatorcontrib>Hoe, Susan Ling Ling</creatorcontrib><creatorcontrib>Marzuki, Marini</creatorcontrib><creatorcontrib>Soo‐Beng Khoo, Alan</creatorcontrib><creatorcontrib>Sriram, Sharath</creatorcontrib><creatorcontrib>Ostrikov, Kostya (Ken)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chan, Jun Yuan</au><au>Ahmad Kayani, Aminuddin Bin</au><au>Md Ali, Mohd Anuar</au><au>Kok, Chee Kuang</au><au>Ramdzan Buyong, Muhamad</au><au>Hoe, Susan Ling Ling</au><au>Marzuki, Marini</au><au>Soo‐Beng Khoo, Alan</au><au>Sriram, Sharath</au><au>Ostrikov, Kostya (Ken)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dielectrophoretic deformation of breast cancer cells for lab on a chip applications</atitle><jtitle>Electrophoresis</jtitle><addtitle>Electrophoresis</addtitle><date>2019-10</date><risdate>2019</risdate><volume>40</volume><issue>20</issue><spage>2728</spage><epage>2735</epage><pages>2728-2735</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA‐MB‐231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA‐MB‐231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA‐MB‐231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. 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| subjects | Blebbing Breast cancer Breast Neoplasms - pathology Cancer Cell Line, Tumor Cell Membrane - physiology Cell Shape - physiology Cell shrinkage Deformation Dielectrophoresis Electric fields Electrophoresis - instrumentation Electrophoresis - methods Female Finite element method Humans Low conductivity Microelectrodes Microfluidics Shrinkage |
| title | Dielectrophoretic deformation of breast cancer cells for lab on a chip applications |
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