Finding of bands of higher molecular weight than expected in three proteins in bovine preimplantation embryos
We report here the existence of bands of higher molecular weight after western blot analysis in three proteins – Skp1, p27 and IκBα in bovine preimplantation embryos. This finding is specific to preimplantation embryos (from the 2-cell stage to the blastocyst stage) and not differentiated fibroblast...
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| Veröffentlicht in: | Zygote (Cambridge) 2019-06, Vol.27 (3), p.187-189 |
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| container_title | Zygote (Cambridge) |
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| creator | Kinterova, Veronika Petruskova, Veronika Kanka, Jiri Toralova, Tereza |
| description | We report here the existence of bands of higher molecular weight after western blot analysis in three proteins – Skp1, p27 and IκBα in bovine preimplantation embryos. This finding is specific to preimplantation embryos (from the 2-cell stage to the blastocyst stage) and not differentiated fibroblast cells in which these bands were of expected molecular weight. We suggest that these bands of higher molecular weight represent a complex of proteins that are characteristic of preimplantation embryos. |
| doi_str_mv | 10.1017/S0967199419000133 |
| format | Article |
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| subjects | Animals Blastocyst - cytology Blastocyst - metabolism Blotting, Western Cattle Cell cycle Cell differentiation Cyclin-Dependent Kinase Inhibitor p27 - chemistry Cyclin-Dependent Kinase Inhibitor p27 - metabolism Embryonic Development Embryos Female Fibroblasts In vitro fertilization Molecular Weight NF-KappaB Inhibitor alpha - chemistry NF-KappaB Inhibitor alpha - metabolism Proteins Proteins - chemistry Proteins - metabolism S-Phase Kinase-Associated Proteins - chemistry S-Phase Kinase-Associated Proteins - metabolism Short Communication |
| title | Finding of bands of higher molecular weight than expected in three proteins in bovine preimplantation embryos |
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