MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices

Sample multiplexing facilitates scRNA-seq by reducing costs and identifying artifacts such as cell doublets. However, universal and scalable sample barcoding strategies have not been described. We therefore developed MULTI-seq: multiplexing using lipid-tagged indices for single-cell and single-nucle...

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Veröffentlicht in:	Nature methods 2019-07, Vol.16 (7), p.619-626
Hauptverfasser:	McGinnis, Christopher S., Patterson, David M., Winkler, Juliane, Conrad, Daniel N., Hein, Marco Y., Srivastava, Vasudha, Hu, Jennifer L., Murrow, Lyndsay M., Weissman, Jonathan S., Werb, Zena, Chow, Eric D., Gartner, Zev J.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2017 631/208/199 631/337/2019 Analysis Animals Artifact identification Base Sequence Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Breast cancer Cancer Cell activation Cell viability Cryopreservation Epithelial cells Gene expression Gene sequencing Genetic aspects Health aspects HEK293 Cells High-Throughput Nucleotide Sequencing Humans Life Sciences Lipids Lipids - chemistry Lymphocytes T Mammary gland Metastases Methods Molecular dynamics Multiplexing Nuclei (cytology) Oncology, Experimental Perturbation Proteomics Reagents Ribonucleic acid RNA RNA sequencing Sequence Analysis, RNA - methods Single-Cell Analysis - methods Tagging Xenografts Xenotransplantation
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