Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates
Aim Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt. Methods and Results Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin...
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| Veröffentlicht in: | Journal of applied microbiology 2019-09, Vol.127 (3), p.693-700 |
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| container_title | Journal of applied microbiology |
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| creator | Mahmoud, B.S. ElMasry, S.A. Fahim, N.A.E.M.M. Abd ElSattar, M.A. Shaker, O.A. |
| description | Aim
Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt.
Methods and Results
Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates of Staphylococci by BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT), agar dilution with the addition of MTT and triphenyl tetrazolium chloride at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. BMD (MTT) showed the highest agreement in comparison with the standard BMD.
Conclusions
Colorimetric BMD was rapid and easy to interpret. Colorimetric agar dilution (MTT) was less tedious than BMD.
Significance and Impact of the Study
Colorimetric antibiotic susceptibility is a good option to provide rapid reliable results for critically ill patients. In addition, agar dilution (MTT) helps to investigate outbreaks of methicillin‐resistant Staphylococcus aureus (MRSA), VISA or VRSA. BMD (MTT) can be performed routinely to detect VA MIC in MRSA blood stream infections and hospital acquired pneumonia, where, high VA MIC is associated with a higher mortality rate. |
| doi_str_mv | 10.1111/jam.14347 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2242817953</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2268950532</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3537-fa83b4b512af5af277d7b63641fac9a3dc866a16729a76ca19aab2db1a11f3483</originalsourceid><addsrcrecordid>eNp1kUtPxCAUhYnR-Bhd-AdMEze66FigQLucjO9o3Oi6uWVoZEJhBBrTfy8zoy5MZAPkfjm55xyETnExxelcLaGf4pKWYgcdYspZTrggu5t3mbNCkAN0FMKyKDAtGN9HBxSTgtesOET-WkUlo3Y2c10GNupWu6hlFoYg1Wr9NTqOWTtm0hnnda-iT-NeW90Pfabte0Ki8-u5lcpGDxs1bbMQYfU-GiedlGAyHZyBqMIx2uvABHXyfU_Q2-3N6_w-f3q5e5jPnnJJGRV5BxVty5ZhAh2DjgixEC1PjnAHsga6kBXngJPTGgSXgGuAlixaDBh3tKzoBF1sdVfefQwqxKbXyZMxYJUbQkNISSosakYTev4HXbrB27RdoniVkmKUJOpyS0nvQvCqa1YpD_Bjg4tmXUSTimg2RST27FtxaHu1-CV_kk_A1Rb41EaN_ys1j7PnreQXzdeUqw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2268950532</pqid></control><display><type>article</type><title>Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Mahmoud, B.S. ; ElMasry, S.A. ; Fahim, N.A.E.M.M. ; Abd ElSattar, M.A. ; Shaker, O.A.</creator><creatorcontrib>Mahmoud, B.S. ; ElMasry, S.A. ; Fahim, N.A.E.M.M. ; Abd ElSattar, M.A. ; Shaker, O.A.</creatorcontrib><description>Aim
Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt.
Methods and Results
Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates of Staphylococci by BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT), agar dilution with the addition of MTT and triphenyl tetrazolium chloride at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. BMD (MTT) showed the highest agreement in comparison with the standard BMD.
Conclusions
Colorimetric BMD was rapid and easy to interpret. Colorimetric agar dilution (MTT) was less tedious than BMD.
Significance and Impact of the Study
Colorimetric antibiotic susceptibility is a good option to provide rapid reliable results for critically ill patients. In addition, agar dilution (MTT) helps to investigate outbreaks of methicillin‐resistant Staphylococcus aureus (MRSA), VISA or VRSA. BMD (MTT) can be performed routinely to detect VA MIC in MRSA blood stream infections and hospital acquired pneumonia, where, high VA MIC is associated with a higher mortality rate.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14347</identifier><identifier>PMID: 31206950</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Antibiotics ; Antimicrobial agents ; Ciprofloxacin ; Clindamycin ; colorimetric assay ; Colorimetry ; Dilution ; dimethyl thiazole diphenyl tetrazolium bromide ; Drug resistance ; Erythromycin ; Gentamicin ; Linezolid ; Methicillin ; Minimum inhibitory concentration ; Outbreaks ; Oxacillin ; Staphylococcus aureus ; Staphylococcus infections ; Staphylococcus MIC ; susceptibility tests ; Tetrazolium salt ; triphenyl tetrazolium chloride ; Triphenyltetrazolium chloride ; Vancomycin</subject><ispartof>Journal of applied microbiology, 2019-09, Vol.127 (3), p.693-700</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-fa83b4b512af5af277d7b63641fac9a3dc866a16729a76ca19aab2db1a11f3483</citedby><cites>FETCH-LOGICAL-c3537-fa83b4b512af5af277d7b63641fac9a3dc866a16729a76ca19aab2db1a11f3483</cites><orcidid>0000-0001-6388-3857 ; 0000-0002-9968-3301</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14347$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14347$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31206950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahmoud, B.S.</creatorcontrib><creatorcontrib>ElMasry, S.A.</creatorcontrib><creatorcontrib>Fahim, N.A.E.M.M.</creatorcontrib><creatorcontrib>Abd ElSattar, M.A.</creatorcontrib><creatorcontrib>Shaker, O.A.</creatorcontrib><title>Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt.
Methods and Results
Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates of Staphylococci by BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT), agar dilution with the addition of MTT and triphenyl tetrazolium chloride at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. BMD (MTT) showed the highest agreement in comparison with the standard BMD.
Conclusions
Colorimetric BMD was rapid and easy to interpret. Colorimetric agar dilution (MTT) was less tedious than BMD.
Significance and Impact of the Study
Colorimetric antibiotic susceptibility is a good option to provide rapid reliable results for critically ill patients. In addition, agar dilution (MTT) helps to investigate outbreaks of methicillin‐resistant Staphylococcus aureus (MRSA), VISA or VRSA. BMD (MTT) can be performed routinely to detect VA MIC in MRSA blood stream infections and hospital acquired pneumonia, where, high VA MIC is associated with a higher mortality rate.</description><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Ciprofloxacin</subject><subject>Clindamycin</subject><subject>colorimetric assay</subject><subject>Colorimetry</subject><subject>Dilution</subject><subject>dimethyl thiazole diphenyl tetrazolium bromide</subject><subject>Drug resistance</subject><subject>Erythromycin</subject><subject>Gentamicin</subject><subject>Linezolid</subject><subject>Methicillin</subject><subject>Minimum inhibitory concentration</subject><subject>Outbreaks</subject><subject>Oxacillin</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Staphylococcus MIC</subject><subject>susceptibility tests</subject><subject>Tetrazolium salt</subject><subject>triphenyl tetrazolium chloride</subject><subject>Triphenyltetrazolium chloride</subject><subject>Vancomycin</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kUtPxCAUhYnR-Bhd-AdMEze66FigQLucjO9o3Oi6uWVoZEJhBBrTfy8zoy5MZAPkfjm55xyETnExxelcLaGf4pKWYgcdYspZTrggu5t3mbNCkAN0FMKyKDAtGN9HBxSTgtesOET-WkUlo3Y2c10GNupWu6hlFoYg1Wr9NTqOWTtm0hnnda-iT-NeW90Pfabte0Ki8-u5lcpGDxs1bbMQYfU-GiedlGAyHZyBqMIx2uvABHXyfU_Q2-3N6_w-f3q5e5jPnnJJGRV5BxVty5ZhAh2DjgixEC1PjnAHsga6kBXngJPTGgSXgGuAlixaDBh3tKzoBF1sdVfefQwqxKbXyZMxYJUbQkNISSosakYTev4HXbrB27RdoniVkmKUJOpyS0nvQvCqa1YpD_Bjg4tmXUSTimg2RST27FtxaHu1-CV_kk_A1Rb41EaN_ys1j7PnreQXzdeUqw</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Mahmoud, B.S.</creator><creator>ElMasry, S.A.</creator><creator>Fahim, N.A.E.M.M.</creator><creator>Abd ElSattar, M.A.</creator><creator>Shaker, O.A.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6388-3857</orcidid><orcidid>https://orcid.org/0000-0002-9968-3301</orcidid></search><sort><creationdate>201909</creationdate><title>Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates</title><author>Mahmoud, B.S. ; ElMasry, S.A. ; Fahim, N.A.E.M.M. ; Abd ElSattar, M.A. ; Shaker, O.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-fa83b4b512af5af277d7b63641fac9a3dc866a16729a76ca19aab2db1a11f3483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Ciprofloxacin</topic><topic>Clindamycin</topic><topic>colorimetric assay</topic><topic>Colorimetry</topic><topic>Dilution</topic><topic>dimethyl thiazole diphenyl tetrazolium bromide</topic><topic>Drug resistance</topic><topic>Erythromycin</topic><topic>Gentamicin</topic><topic>Linezolid</topic><topic>Methicillin</topic><topic>Minimum inhibitory concentration</topic><topic>Outbreaks</topic><topic>Oxacillin</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Staphylococcus MIC</topic><topic>susceptibility tests</topic><topic>Tetrazolium salt</topic><topic>triphenyl tetrazolium chloride</topic><topic>Triphenyltetrazolium chloride</topic><topic>Vancomycin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahmoud, B.S.</creatorcontrib><creatorcontrib>ElMasry, S.A.</creatorcontrib><creatorcontrib>Fahim, N.A.E.M.M.</creatorcontrib><creatorcontrib>Abd ElSattar, M.A.</creatorcontrib><creatorcontrib>Shaker, O.A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahmoud, B.S.</au><au>ElMasry, S.A.</au><au>Fahim, N.A.E.M.M.</au><au>Abd ElSattar, M.A.</au><au>Shaker, O.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-09</date><risdate>2019</risdate><volume>127</volume><issue>3</issue><spage>693</spage><epage>700</epage><pages>693-700</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt.
Methods and Results
Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates of Staphylococci by BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT), agar dilution with the addition of MTT and triphenyl tetrazolium chloride at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. BMD (MTT) showed the highest agreement in comparison with the standard BMD.
Conclusions
Colorimetric BMD was rapid and easy to interpret. Colorimetric agar dilution (MTT) was less tedious than BMD.
Significance and Impact of the Study
Colorimetric antibiotic susceptibility is a good option to provide rapid reliable results for critically ill patients. In addition, agar dilution (MTT) helps to investigate outbreaks of methicillin‐resistant Staphylococcus aureus (MRSA), VISA or VRSA. BMD (MTT) can be performed routinely to detect VA MIC in MRSA blood stream infections and hospital acquired pneumonia, where, high VA MIC is associated with a higher mortality rate.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31206950</pmid><doi>10.1111/jam.14347</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6388-3857</orcidid><orcidid>https://orcid.org/0000-0002-9968-3301</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2019-09, Vol.127 (3), p.693-700 |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete |
| subjects | Antibiotics Antimicrobial agents Ciprofloxacin Clindamycin colorimetric assay Colorimetry Dilution dimethyl thiazole diphenyl tetrazolium bromide Drug resistance Erythromycin Gentamicin Linezolid Methicillin Minimum inhibitory concentration Outbreaks Oxacillin Staphylococcus aureus Staphylococcus infections Staphylococcus MIC susceptibility tests Tetrazolium salt triphenyl tetrazolium chloride Triphenyltetrazolium chloride Vancomycin |
| title | Detection of antibiotic susceptibility by colorimetric minimum inhibitory concentration in staphylococcal isolates |
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