Isolation and purification of colicin ECL 12, a bacteriocin that inhibits strains of Escherichia coli O157:H7
A swine fecal isolate, identified as Escherichia coli ECL12, was found to produce an antimicrobial substance designated as colicin ECL12. Colicin ECL12 was inhibitory against 20 strains of E. coli O157:H7 previously isolated from both human and bovine feces. Identification of the producer strain was...
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| Veröffentlicht in: | Journal of food protection 1997-12, Vol.60 (12), p.1520-1528 |
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| container_end_page | 1528 |
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| container_issue | 12 |
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| container_title | Journal of food protection |
| container_volume | 60 |
| creator | Lyon, W.J. (Louisiana State University Agricultural Center, Louisiana State University, Baton Rouge, LA.) Olson, D.G |
| description | A swine fecal isolate, identified as Escherichia coli ECL12, was found to produce an antimicrobial substance designated as colicin ECL12. Colicin ECL12 was inhibitory against 20 strains of E. coli O157:H7 previously isolated from both human and bovine feces. Identification of the producer strain was determined phenotypically by biochemical and morphological tests. Colicin ECL12 was sensitive to several proteolytic enzymes. Adsorption of colicin ECL12 to sensitive cells of E. coli O157:H7 was bactericidal, resulting in a 2 log reduction in viable cell counts. Colicin ECL12 was purified from strain ECL12 by cell extraction and ion-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of colicin ECL12 resolved a single protein with a molecular weight of approximately 65,000 |
| doi_str_mv | 10.4315/0362-028X-60.12.1520 |
| format | Article |
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(Louisiana State University Agricultural Center, Louisiana State University, Baton Rouge, LA.) ; Olson, D.G</creator><creatorcontrib>Lyon, W.J. (Louisiana State University Agricultural Center, Louisiana State University, Baton Rouge, LA.) ; Olson, D.G</creatorcontrib><description>A swine fecal isolate, identified as Escherichia coli ECL12, was found to produce an antimicrobial substance designated as colicin ECL12. Colicin ECL12 was inhibitory against 20 strains of E. coli O157:H7 previously isolated from both human and bovine feces. Identification of the producer strain was determined phenotypically by biochemical and morphological tests. Colicin ECL12 was sensitive to several proteolytic enzymes. Adsorption of colicin ECL12 to sensitive cells of E. coli O157:H7 was bactericidal, resulting in a 2 log reduction in viable cell counts. Colicin ECL12 was purified from strain ECL12 by cell extraction and ion-exchange chromatography. 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Psychology ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; PRESERVADORES ; PRESERVATIVES ; PROPIEDADES ANTIMICROBIANAS ; PROPRIETE ANTIMICROBIENNE</subject><ispartof>Journal of food protection, 1997-12, Vol.60 (12), p.1520-1528</ispartof><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-c3ae4053eb7c6993b863de722c4baa5535f013c84f52e64d3766dd02ce92670b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924,64386</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2127289$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31207744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lyon, W.J. (Louisiana State University Agricultural Center, Louisiana State University, Baton Rouge, LA.)</creatorcontrib><creatorcontrib>Olson, D.G</creatorcontrib><title>Isolation and purification of colicin ECL 12, a bacteriocin that inhibits strains of Escherichia coli O157:H7</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>A swine fecal isolate, identified as Escherichia coli ECL12, was found to produce an antimicrobial substance designated as colicin ECL12. Colicin ECL12 was inhibitory against 20 strains of E. coli O157:H7 previously isolated from both human and bovine feces. Identification of the producer strain was determined phenotypically by biochemical and morphological tests. Colicin ECL12 was sensitive to several proteolytic enzymes. Adsorption of colicin ECL12 to sensitive cells of E. coli O157:H7 was bactericidal, resulting in a 2 log reduction in viable cell counts. Colicin ECL12 was purified from strain ECL12 by cell extraction and ion-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of colicin ECL12 resolved a single protein with a molecular weight of approximately 65,000</description><subject>AGENT DE CONSERVATION</subject><subject>ANTIMICROBIAL PROPERTIES</subject><subject>ANTIMICROBIAL PROTEINS</subject><subject>BACTERIA COLIFORME</subject><subject>BACTERIE COLIFORME</subject><subject>BACTERIOCINAS</subject><subject>BACTERIOCINE</subject><subject>BACTERIOCINS</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>COLIFORM BACTERIA</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>FOOD PRESERVATIVES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>PRESERVADORES</subject><subject>PRESERVATIVES</subject><subject>PROPIEDADES ANTIMICROBIANAS</subject><subject>PROPRIETE ANTIMICROBIENNE</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp90cFuEzEQBmALUdHQ8gaAfECIAxvssdde94aiQCtF6qFU4mbNer3EaLMO9ubA29fbhBw5WRp9_y95hpC3nC2l4PUXJhRUDJqflWJLDkteA3tBFtxIWRlm9EuyOJNL8jrn34wxMKBekUvBgWkt5YLs7nIccApxpDh2dH9IoQ_uOIg9dXEILox0vdpQDp8p0hbd5FOI83Ta4kTDuA1tmDLNU8Iw5jm2zm5bkNsGfK6g97zWN7f6mlz0OGT_5vRekcdv6x-r22pz__1u9XVTOSnEVDmBXrJa-FY7ZYxoGyU6rwGcbBHrWtQ948I1sq_BK9kJrVTXMXC-fE-zVlyRT8fefYp_Dj5Pdhey88OAo4-HbAEkNFxJrQr9-F_6rJRqCpRH6FLMOfne7lPYYfprObPzRey8bjuv2ypmOdj5IiX2_tR_aHe-O4f-naCADyeA2eHQJxxdyGcHHDQ0prB3R9ZjtPgrFfL4wI3RpUXrRjwBIwqaUw</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Lyon, W.J. 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Psychology</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>PRESERVADORES</topic><topic>PRESERVATIVES</topic><topic>PROPIEDADES ANTIMICROBIANAS</topic><topic>PROPRIETE ANTIMICROBIENNE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lyon, W.J. 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Colicin ECL12 was sensitive to several proteolytic enzymes. Adsorption of colicin ECL12 to sensitive cells of E. coli O157:H7 was bactericidal, resulting in a 2 log reduction in viable cell counts. Colicin ECL12 was purified from strain ECL12 by cell extraction and ion-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of colicin ECL12 resolved a single protein with a molecular weight of approximately 65,000</abstract><cop>Des Moines, IA</cop><pub>International Association of Milk, Food and Environmental Sanitarians</pub><pmid>31207744</pmid><doi>10.4315/0362-028X-60.12.1520</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of food protection, 1997-12, Vol.60 (12), p.1520-1528 |
| issn | 0362-028X 1944-9097 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection |
| subjects | AGENT DE CONSERVATION ANTIMICROBIAL PROPERTIES ANTIMICROBIAL PROTEINS BACTERIA COLIFORME BACTERIE COLIFORME BACTERIOCINAS BACTERIOCINE BACTERIOCINS Bacteriology Biological and medical sciences COLIFORM BACTERIA Food industries Food microbiology FOOD PRESERVATIVES Fundamental and applied biological sciences. Psychology Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains PRESERVADORES PRESERVATIVES PROPIEDADES ANTIMICROBIANAS PROPRIETE ANTIMICROBIENNE |
| title | Isolation and purification of colicin ECL 12, a bacteriocin that inhibits strains of Escherichia coli O157:H7 |
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