Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging
The abnormal concentration of lysozyme in body fluids and tissues is a potential indicator for diseases such as leukemia and meningitis. In this work, by combining the excellent optical properties of carbon dots (CDs) with the favorable selectivity of molecularly imprinted polymer (MIP), a novel flu...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2019-09, Vol.411 (22), p.5799-5807 |
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| creator | Fang, Mengyao Zhuo, Kelei Chen, Yujuan Zhao, Yajing Bai, Guangyue Wang, Jianji |
| description | The abnormal concentration of lysozyme in body fluids and tissues is a potential indicator for diseases such as leukemia and meningitis. In this work, by combining the excellent optical properties of carbon dots (CDs) with the favorable selectivity of molecularly imprinted polymer (MIP), a novel fluorescent probe for lysozyme detection and cell imaging was constructed, where silanized CDs with low cytotoxicity (CDs/SiO
2
) were used as the fluorescence signal reporter and
N
-isopropylacrylamide (NIPAM) was used as the temperature-sensitive monomer. The as-prepared CDs/SiO
2
/MIP showed a thermo-sensitive property for the response to lysozyme. Moreover, this probe could be used for quantitative detection of lysozyme, with a wider detection range (0.001~0.01 mg/mL), a low detection limit (0.55 μg/mL), and a high selectivity. Importantly, the MTT assay testified that the fluorescent CDs/SiO
2
/MIP probe had low cytotoxicity. In addition, human hepatoma carcinoma cells (HepG-2 cells) could be stained by the CDs/SiO
2
/MIP, and showed a bright intracellular green fluorescence, indicating that the imaging of live cells was possible. This study provides a new way to detect lysozyme in vitro and an attractive perspective to probe intracellular lysozyme in vivo. |
| doi_str_mv | 10.1007/s00216-019-01960-6 |
| format | Article |
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2
) were used as the fluorescence signal reporter and
N
-isopropylacrylamide (NIPAM) was used as the temperature-sensitive monomer. The as-prepared CDs/SiO
2
/MIP showed a thermo-sensitive property for the response to lysozyme. Moreover, this probe could be used for quantitative detection of lysozyme, with a wider detection range (0.001~0.01 mg/mL), a low detection limit (0.55 μg/mL), and a high selectivity. Importantly, the MTT assay testified that the fluorescent CDs/SiO
2
/MIP probe had low cytotoxicity. In addition, human hepatoma carcinoma cells (HepG-2 cells) could be stained by the CDs/SiO
2
/MIP, and showed a bright intracellular green fluorescence, indicating that the imaging of live cells was possible. This study provides a new way to detect lysozyme in vitro and an attractive perspective to probe intracellular lysozyme in vivo.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-019-01960-6</identifier><identifier>PMID: 31209550</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Binding, Competitive ; Biochemistry ; Biocompatibility ; Body fluids ; Carbon - chemistry ; Carbon dots ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Cytotoxicity ; Fluorescence ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Food Science ; Hep G2 Cells ; Hepatoma ; Humans ; Imaging ; Intracellular ; Isopropylacrylamide ; Journalistic ethics ; Laboratory Medicine ; Leukemia ; Limit of Detection ; Lysozyme ; Meningitis ; Microscopy, Electron, Transmission ; Molecular Imprinting ; Monitoring/Environmental Analysis ; Muramidase - analysis ; Nanoparticles - chemistry ; Optical properties ; Polymer industry ; Polymers ; Polymers - chemistry ; Research Paper ; Selectivity ; Silica ; Silicon dioxide ; Silicon Dioxide - chemistry ; Spectrometry, Fluorescence - methods ; Spectrophotometry, Ultraviolet ; Toxicity</subject><ispartof>Analytical and bioanalytical chemistry, 2019-09, Vol.411 (22), p.5799-5807</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Analytical and Bioanalytical Chemistry is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-fb84dff6cc64f241d0af749292588a799197c031360fd70ba1676850c3327f533</citedby><cites>FETCH-LOGICAL-c442t-fb84dff6cc64f241d0af749292588a799197c031360fd70ba1676850c3327f533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-019-01960-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-019-01960-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31209550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fang, Mengyao</creatorcontrib><creatorcontrib>Zhuo, Kelei</creatorcontrib><creatorcontrib>Chen, Yujuan</creatorcontrib><creatorcontrib>Zhao, Yajing</creatorcontrib><creatorcontrib>Bai, Guangyue</creatorcontrib><creatorcontrib>Wang, Jianji</creatorcontrib><title>Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>The abnormal concentration of lysozyme in body fluids and tissues is a potential indicator for diseases such as leukemia and meningitis. In this work, by combining the excellent optical properties of carbon dots (CDs) with the favorable selectivity of molecularly imprinted polymer (MIP), a novel fluorescent probe for lysozyme detection and cell imaging was constructed, where silanized CDs with low cytotoxicity (CDs/SiO
2
) were used as the fluorescence signal reporter and
N
-isopropylacrylamide (NIPAM) was used as the temperature-sensitive monomer. The as-prepared CDs/SiO
2
/MIP showed a thermo-sensitive property for the response to lysozyme. Moreover, this probe could be used for quantitative detection of lysozyme, with a wider detection range (0.001~0.01 mg/mL), a low detection limit (0.55 μg/mL), and a high selectivity. Importantly, the MTT assay testified that the fluorescent CDs/SiO
2
/MIP probe had low cytotoxicity. In addition, human hepatoma carcinoma cells (HepG-2 cells) could be stained by the CDs/SiO
2
/MIP, and showed a bright intracellular green fluorescence, indicating that the imaging of live cells was possible. This study provides a new way to detect lysozyme in vitro and an attractive perspective to probe intracellular lysozyme in vivo.</description><subject>Analytical Chemistry</subject><subject>Binding, Competitive</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Body fluids</subject><subject>Carbon - chemistry</subject><subject>Carbon dots</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytotoxicity</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Food Science</subject><subject>Hep G2 Cells</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Imaging</subject><subject>Intracellular</subject><subject>Isopropylacrylamide</subject><subject>Journalistic ethics</subject><subject>Laboratory Medicine</subject><subject>Leukemia</subject><subject>Limit of Detection</subject><subject>Lysozyme</subject><subject>Meningitis</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Imprinting</subject><subject>Monitoring/Environmental Analysis</subject><subject>Muramidase - analysis</subject><subject>Nanoparticles - chemistry</subject><subject>Optical properties</subject><subject>Polymer industry</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Research Paper</subject><subject>Selectivity</subject><subject>Silica</subject><subject>Silicon dioxide</subject><subject>Silicon Dioxide - chemistry</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Toxicity</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1rFTEUhoMotlb_gAsJuHEzvScfk0mWpbQqFNzoOmTycUnJTK7JzOL66814a4siEsJJwvO-nJMXobcELgnAsKsAlIgOiNq2gE48Q-dEENlR0cPzxzOnZ-hVrfcApJdEvERnjFBQfQ_nqN6mNRdfrZ8XfCh59Hg01TucZ2xNGVtxeam7GlO0Zjfl5O2aTElHHKdDifPS2ENOx8kXHHLB6Vjzj3bDzi_eLrEZmNlh61NqCrOP8_41ehFMqv7NQ71A325vvl5_6u6-fPx8fXXXWc7p0oVRcheCsFbwQDlxYMLAFVW0l9IMShE1WGCECQhugNEQMQjZg2WMDqFn7AJ9OPm2ub6vvi56inVrxMw-r1VTyqkkRHDS0Pd_ofd5LXPrbqOIZAyYfKL2Jnkd55CXYuxmqq96NYCkXPWNuvwH1ZbzU7R59iG29z8E9CSwJddafNDtYydTjpqA3pLWp6R1S1n_SlqLJnr30PE6Tt49Sn5H2wB2AuoW096Xp5H-Y_sT_s6ybA</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Fang, Mengyao</creator><creator>Zhuo, Kelei</creator><creator>Chen, Yujuan</creator><creator>Zhao, Yajing</creator><creator>Bai, Guangyue</creator><creator>Wang, Jianji</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20190901</creationdate><title>Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging</title><author>Fang, Mengyao ; Zhuo, Kelei ; Chen, Yujuan ; Zhao, Yajing ; Bai, Guangyue ; Wang, Jianji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-fb84dff6cc64f241d0af749292588a799197c031360fd70ba1676850c3327f533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical Chemistry</topic><topic>Binding, Competitive</topic><topic>Biochemistry</topic><topic>Biocompatibility</topic><topic>Body fluids</topic><topic>Carbon - 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Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Mengyao</au><au>Zhuo, Kelei</au><au>Chen, Yujuan</au><au>Zhao, Yajing</au><au>Bai, Guangyue</au><au>Wang, Jianji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>411</volume><issue>22</issue><spage>5799</spage><epage>5807</epage><pages>5799-5807</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The abnormal concentration of lysozyme in body fluids and tissues is a potential indicator for diseases such as leukemia and meningitis. In this work, by combining the excellent optical properties of carbon dots (CDs) with the favorable selectivity of molecularly imprinted polymer (MIP), a novel fluorescent probe for lysozyme detection and cell imaging was constructed, where silanized CDs with low cytotoxicity (CDs/SiO
2
) were used as the fluorescence signal reporter and
N
-isopropylacrylamide (NIPAM) was used as the temperature-sensitive monomer. The as-prepared CDs/SiO
2
/MIP showed a thermo-sensitive property for the response to lysozyme. Moreover, this probe could be used for quantitative detection of lysozyme, with a wider detection range (0.001~0.01 mg/mL), a low detection limit (0.55 μg/mL), and a high selectivity. Importantly, the MTT assay testified that the fluorescent CDs/SiO
2
/MIP probe had low cytotoxicity. In addition, human hepatoma carcinoma cells (HepG-2 cells) could be stained by the CDs/SiO
2
/MIP, and showed a bright intracellular green fluorescence, indicating that the imaging of live cells was possible. This study provides a new way to detect lysozyme in vitro and an attractive perspective to probe intracellular lysozyme in vivo.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31209550</pmid><doi>10.1007/s00216-019-01960-6</doi><tpages>9</tpages></addata></record> |
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| subjects | Analytical Chemistry Binding, Competitive Biochemistry Biocompatibility Body fluids Carbon - chemistry Carbon dots Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Cytotoxicity Fluorescence Fluorescent Dyes - chemistry Fluorescent indicators Food Science Hep G2 Cells Hepatoma Humans Imaging Intracellular Isopropylacrylamide Journalistic ethics Laboratory Medicine Leukemia Limit of Detection Lysozyme Meningitis Microscopy, Electron, Transmission Molecular Imprinting Monitoring/Environmental Analysis Muramidase - analysis Nanoparticles - chemistry Optical properties Polymer industry Polymers Polymers - chemistry Research Paper Selectivity Silica Silicon dioxide Silicon Dioxide - chemistry Spectrometry, Fluorescence - methods Spectrophotometry, Ultraviolet Toxicity |
| title | Fluorescent probe based on carbon dots/silica/molecularly imprinted polymer for lysozyme detection and cell imaging |
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