DLX3 regulates osteogenic differentiation of bone marrow mesenchymal stem cells via Wnt/β-catenin pathway mediated histone methylation of DKK4
Distal-less homeobox 3 (DLX3) is an important transcription factor involved in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). However, the underlying mechanism is not clear. This study investigated the underlying mechanism of DLX3 in osteogenic differentiation. DLX3 ov...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2019-08, Vol.516 (1), p.171-176 |
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| creator | Sun, Shichen Yu, Miao Fan, Zhuangzhuang Yeh, I-Ting Feng, Hailan Liu, Haochen Han, Dong |
| description | Distal-less homeobox 3 (DLX3) is an important transcription factor involved in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). However, the underlying mechanism is not clear. This study investigated the underlying mechanism of DLX3 in osteogenic differentiation.
DLX3 overexpression and knockdown in cells were achieved using lentiviruses. The osteogenic differentiation of BMSCs was detected using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction (RT-qPCR), Western blotting, and chromatin immunoprecipitation (ChIP) assays.
DLX3 overexpression promoted the osteogenic differentiation of BMSCs, whereas DLX3 knockdown reduced the osteogenic differentiation of BMSCs. RT-qPCR and Western blotting assays showed that DLX3 modulated osteogenic differentiation via the Wnt/β-catenin pathway. ChIP-qPCR showed that DLX3 knockdown promoted DKK4 expression by decreasing the enrichment of histone H3 lysine 27 trimethylation (H3K27me3) in the promotor region of DKK4.
Our data implied that DLX3 regulated Wnt/β-catenin pathway through histone modification of DKK4 during the osteogenic differentiation of BMSCs.
•We explored the mechanism by which DLX3 regulates osteogenesis.•DLX3 regulates the osteogenic differentiation of BMSCs via the Wnt/β-catenin pathway.•DKK4 histone methylation is negatively regulated by DLX3, contributing to osteogenesis. |
| doi_str_mv | 10.1016/j.bbrc.2019.06.029 |
| format | Article |
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DLX3 overexpression and knockdown in cells were achieved using lentiviruses. The osteogenic differentiation of BMSCs was detected using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction (RT-qPCR), Western blotting, and chromatin immunoprecipitation (ChIP) assays.
DLX3 overexpression promoted the osteogenic differentiation of BMSCs, whereas DLX3 knockdown reduced the osteogenic differentiation of BMSCs. RT-qPCR and Western blotting assays showed that DLX3 modulated osteogenic differentiation via the Wnt/β-catenin pathway. ChIP-qPCR showed that DLX3 knockdown promoted DKK4 expression by decreasing the enrichment of histone H3 lysine 27 trimethylation (H3K27me3) in the promotor region of DKK4.
Our data implied that DLX3 regulated Wnt/β-catenin pathway through histone modification of DKK4 during the osteogenic differentiation of BMSCs.
•We explored the mechanism by which DLX3 regulates osteogenesis.•DLX3 regulates the osteogenic differentiation of BMSCs via the Wnt/β-catenin pathway.•DKK4 histone methylation is negatively regulated by DLX3, contributing to osteogenesis.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2019.06.029</identifier><identifier>PMID: 31202458</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>DKK4 ; DLX3 ; Epigenetic regulation ; Histone methylation ; Osteogenic differentiation</subject><ispartof>Biochemical and biophysical research communications, 2019-08, Vol.516 (1), p.171-176</ispartof><rights>2019 The Authors</rights><rights>Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-a46eef72b45e0bfa51d2ae71b31851c105861ea9ba95ad44fbb74bf2d70ec80b3</citedby><cites>FETCH-LOGICAL-c400t-a46eef72b45e0bfa51d2ae71b31851c105861ea9ba95ad44fbb74bf2d70ec80b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2019.06.029$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31202458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Shichen</creatorcontrib><creatorcontrib>Yu, Miao</creatorcontrib><creatorcontrib>Fan, Zhuangzhuang</creatorcontrib><creatorcontrib>Yeh, I-Ting</creatorcontrib><creatorcontrib>Feng, Hailan</creatorcontrib><creatorcontrib>Liu, Haochen</creatorcontrib><creatorcontrib>Han, Dong</creatorcontrib><title>DLX3 regulates osteogenic differentiation of bone marrow mesenchymal stem cells via Wnt/β-catenin pathway mediated histone methylation of DKK4</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Distal-less homeobox 3 (DLX3) is an important transcription factor involved in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). However, the underlying mechanism is not clear. This study investigated the underlying mechanism of DLX3 in osteogenic differentiation.
DLX3 overexpression and knockdown in cells were achieved using lentiviruses. The osteogenic differentiation of BMSCs was detected using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction (RT-qPCR), Western blotting, and chromatin immunoprecipitation (ChIP) assays.
DLX3 overexpression promoted the osteogenic differentiation of BMSCs, whereas DLX3 knockdown reduced the osteogenic differentiation of BMSCs. RT-qPCR and Western blotting assays showed that DLX3 modulated osteogenic differentiation via the Wnt/β-catenin pathway. ChIP-qPCR showed that DLX3 knockdown promoted DKK4 expression by decreasing the enrichment of histone H3 lysine 27 trimethylation (H3K27me3) in the promotor region of DKK4.
Our data implied that DLX3 regulated Wnt/β-catenin pathway through histone modification of DKK4 during the osteogenic differentiation of BMSCs.
•We explored the mechanism by which DLX3 regulates osteogenesis.•DLX3 regulates the osteogenic differentiation of BMSCs via the Wnt/β-catenin pathway.•DKK4 histone methylation is negatively regulated by DLX3, contributing to osteogenesis.</description><subject>DKK4</subject><subject>DLX3</subject><subject>Epigenetic regulation</subject><subject>Histone methylation</subject><subject>Osteogenic differentiation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAYhC0EotvCC3BAPnJJ-v9eJ9lIXFBbCupKvRTRm2U7f7peJfFie1vtU_AufZA-E1629MhpLjOfNDOMfUAoEbA-XZfGBFsKwLaEugTRvmIzhBYKgSBfsxkA1IVo8faIHce4BkCUdfuWHc1RgJDVYsZ-ny9v5zzQ3XbQiSL3MZG_o8lZ3rm-p0BTcjo5P3Hfc-Mn4qMOwT_wkSJNdrUb9cBzaOSWhiHye6f5zymdPj0WNhMnN_GNTqsHvcuJLqOo4ysX018SpdVueKGfX13Jd-xNr4dI75_1hP34enFz9q1YXl9-P_uyLKwESIWWNVHfCCMrAtPrCjuhqUEzx0WFFqFa1Ei6NbqtdCdlb0wjTS-6BsguwMxP2KcDdxP8ry3FpEYX9w30RH4blRBSYNVIlNkqDlYbfIyBerUJLo-wUwhqf4Raq_0Ran-EglrlI3Lo4zN_a3Lvl8i_5bPh88FAueW9o6CidXnQvFEgm1Tn3f_4fwBM5Z1_</recordid><startdate>20190813</startdate><enddate>20190813</enddate><creator>Sun, Shichen</creator><creator>Yu, Miao</creator><creator>Fan, Zhuangzhuang</creator><creator>Yeh, I-Ting</creator><creator>Feng, Hailan</creator><creator>Liu, Haochen</creator><creator>Han, Dong</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190813</creationdate><title>DLX3 regulates osteogenic differentiation of bone marrow mesenchymal stem cells via Wnt/β-catenin pathway mediated histone methylation of DKK4</title><author>Sun, Shichen ; Yu, Miao ; Fan, Zhuangzhuang ; Yeh, I-Ting ; Feng, Hailan ; Liu, Haochen ; Han, Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-a46eef72b45e0bfa51d2ae71b31851c105861ea9ba95ad44fbb74bf2d70ec80b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>DKK4</topic><topic>DLX3</topic><topic>Epigenetic regulation</topic><topic>Histone methylation</topic><topic>Osteogenic differentiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Shichen</creatorcontrib><creatorcontrib>Yu, Miao</creatorcontrib><creatorcontrib>Fan, Zhuangzhuang</creatorcontrib><creatorcontrib>Yeh, I-Ting</creatorcontrib><creatorcontrib>Feng, Hailan</creatorcontrib><creatorcontrib>Liu, Haochen</creatorcontrib><creatorcontrib>Han, Dong</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Shichen</au><au>Yu, Miao</au><au>Fan, Zhuangzhuang</au><au>Yeh, I-Ting</au><au>Feng, Hailan</au><au>Liu, Haochen</au><au>Han, Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DLX3 regulates osteogenic differentiation of bone marrow mesenchymal stem cells via Wnt/β-catenin pathway mediated histone methylation of DKK4</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2019-08-13</date><risdate>2019</risdate><volume>516</volume><issue>1</issue><spage>171</spage><epage>176</epage><pages>171-176</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Distal-less homeobox 3 (DLX3) is an important transcription factor involved in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). However, the underlying mechanism is not clear. This study investigated the underlying mechanism of DLX3 in osteogenic differentiation.
DLX3 overexpression and knockdown in cells were achieved using lentiviruses. The osteogenic differentiation of BMSCs was detected using alkaline phosphatase expression, alizarin red staining, real-time quantitative polymerase chain reaction (RT-qPCR), Western blotting, and chromatin immunoprecipitation (ChIP) assays.
DLX3 overexpression promoted the osteogenic differentiation of BMSCs, whereas DLX3 knockdown reduced the osteogenic differentiation of BMSCs. RT-qPCR and Western blotting assays showed that DLX3 modulated osteogenic differentiation via the Wnt/β-catenin pathway. ChIP-qPCR showed that DLX3 knockdown promoted DKK4 expression by decreasing the enrichment of histone H3 lysine 27 trimethylation (H3K27me3) in the promotor region of DKK4.
Our data implied that DLX3 regulated Wnt/β-catenin pathway through histone modification of DKK4 during the osteogenic differentiation of BMSCs.
•We explored the mechanism by which DLX3 regulates osteogenesis.•DLX3 regulates the osteogenic differentiation of BMSCs via the Wnt/β-catenin pathway.•DKK4 histone methylation is negatively regulated by DLX3, contributing to osteogenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31202458</pmid><doi>10.1016/j.bbrc.2019.06.029</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | DKK4 DLX3 Epigenetic regulation Histone methylation Osteogenic differentiation |
| title | DLX3 regulates osteogenic differentiation of bone marrow mesenchymal stem cells via Wnt/β-catenin pathway mediated histone methylation of DKK4 |
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