Active fusions of Cas9 orthologs

•Cas9 guides catalytic domains to their targets only in certain configurations.•Cas9 orthologs SpCas9, SaCas9 and CjCas9 are active in fusion with DNMT3A.•Other tested orthologs (NmCas9, St1Cas9 and FnCas9) were inactive.•Nuclear localization is critical for activity of Cas9-based fusion proteins. M...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of biotechnology 2019-08, Vol.301, p.18-23
Hauptverfasser:	Josipović, Goran, Zoldoš, Vlatka, Vojta, Aleksandar
Format:	Artikel
Sprache:	eng
Schlagworte:	Cas9 fusion Cas9 ortholog CRISPR-Cas Systems - genetics DNA, Recombinant - genetics DNMT3A Epigenome editing Epigenomics - methods Gene Editing - methods Gene Expression Regulation Gene Fusion - genetics sgRNA
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!