Morphological Changes Induced By Extremely Low‐Frequency Electric Fields
In this paper, morphological effects of electric fields on avian erythrocytes (nucleated red blood cells) have been studied in detail. Morphological changes include rounding and cytoplasm transparency. It has been shown that the effect is non‐thermal. Careful imaging and image analyses have been car...
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| Veröffentlicht in: | Bioelectromagnetics 2019-09, Vol.40 (6), p.375-390 |
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| creator | Imani, Mahdi Kazemi, Sepide Saviz, Mehrdad Farahmand, Leila Sadeghi, Behnam Faraji‐dana, Reza |
| description | In this paper, morphological effects of electric fields on avian erythrocytes (nucleated red blood cells) have been studied in detail. Morphological changes include rounding and cytoplasm transparency. It has been shown that the effect is non‐thermal. Careful imaging and image analyses have been carried out to show that the degree of this effect is frequency‐dependent, and has a higher conversion rate at higher temperatures. Furthermore, to better understand the mechanisms behind the morphological changes, we investigated the dedifferentiation hypothesis and performed a series of tests on avian erythrocytes including fluorescence spectroscopy for hemoglobin, and tests on human umbilical cord blood, mesenchymal stem cells, and bone marrow mesenchymal stem cells including flow‐cytometry analysis for expression of certain markers and calcium staining. Bioelectromagnetics. 2019;40:375–390. © 2019 Bioelectromagnetics Society. |
| doi_str_mv | 10.1002/bem.22195 |
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Morphological changes include rounding and cytoplasm transparency. It has been shown that the effect is non‐thermal. Careful imaging and image analyses have been carried out to show that the degree of this effect is frequency‐dependent, and has a higher conversion rate at higher temperatures. Furthermore, to better understand the mechanisms behind the morphological changes, we investigated the dedifferentiation hypothesis and performed a series of tests on avian erythrocytes including fluorescence spectroscopy for hemoglobin, and tests on human umbilical cord blood, mesenchymal stem cells, and bone marrow mesenchymal stem cells including flow‐cytometry analysis for expression of certain markers and calcium staining. 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Morphological changes include rounding and cytoplasm transparency. It has been shown that the effect is non‐thermal. Careful imaging and image analyses have been carried out to show that the degree of this effect is frequency‐dependent, and has a higher conversion rate at higher temperatures. Furthermore, to better understand the mechanisms behind the morphological changes, we investigated the dedifferentiation hypothesis and performed a series of tests on avian erythrocytes including fluorescence spectroscopy for hemoglobin, and tests on human umbilical cord blood, mesenchymal stem cells, and bone marrow mesenchymal stem cells including flow‐cytometry analysis for expression of certain markers and calcium staining. Bioelectromagnetics. 2019;40:375–390. © 2019 Bioelectromagnetics Society.</description><subject>Bone marrow</subject><subject>Calcium</subject><subject>cell morphology</subject><subject>Cord blood</subject><subject>Cytometry</subject><subject>Cytoplasm</subject><subject>dedifferentiation</subject><subject>electric field</subject><subject>Electric fields</subject><subject>Erythrocytes</subject><subject>extremely low frequency</subject><subject>Fluorescence</subject><subject>Fluorescence spectroscopy</subject><subject>Hemoglobin</subject><subject>Mesenchyme</subject><subject>Morphology</subject><subject>non‐thermal effects</subject><subject>Rounding</subject><subject>Stem cells</subject><subject>Thermal imaging</subject><subject>Umbilical cord</subject><issn>0197-8462</issn><issn>1521-186X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10LFOwzAUBVALgaAUBn4ARWKBIeBnx7Ez0qqFoiKWDmxR7LxCkJMUu1HpxifwjXwJhgIDEtMb3tHV1SXkCOg5UMouNNbnjEEmtkgPBIMYVHq_TXoUMhmrJGV7ZN_7J0qpUpTvkj0OIGTGZI_c3LZu8dja9qEyhY2Gj0XzgD6aNGVnsIwG62j0snRYo11H03b1_vo2dvjcYWPCx6JZuspE4wpt6Q_IzrywHg-_b5_MxqPZ8Dqe3l1NhpfT2HDBRcyl1iItJQUjmDC6BA0CpFagBaNpppVgmcxKlbGC0xRlqko5R2aKJCmE4X1yuolduDYU8cu8rrxBa4sG287njPEkUUqqLNCTP_Sp7VwTygUlQSqQHII62yjjWu8dzvOFq-rCrXOg-ee-edg3_9o32OPvxE7XWP7Kn0EDuNiAVWVx_X9SPhjdbiI_AOk9g0Y</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Imani, Mahdi</creator><creator>Kazemi, Sepide</creator><creator>Saviz, Mehrdad</creator><creator>Farahmand, Leila</creator><creator>Sadeghi, Behnam</creator><creator>Faraji‐dana, Reza</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>Morphological Changes Induced By Extremely Low‐Frequency Electric Fields</title><author>Imani, Mahdi ; 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| subjects | Bone marrow Calcium cell morphology Cord blood Cytometry Cytoplasm dedifferentiation electric field Electric fields Erythrocytes extremely low frequency Fluorescence Fluorescence spectroscopy Hemoglobin Mesenchyme Morphology non‐thermal effects Rounding Stem cells Thermal imaging Umbilical cord |
| title | Morphological Changes Induced By Extremely Low‐Frequency Electric Fields |
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