Liquid mycobacterial culture outcomes after different sputum collection techniques before and during treatment
Mycobacterial sputum culture is a key diagnostic and research tool. To compare mycobacterial culture outcomes of three sputum collection methods. We compared culture results within sets of three sputum samples collected from 18 HIV-infected adult tuberculosis patients at regular intervals up to 84 d...
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2019-05, Vol.116, p.17-21 |
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| creator | Lourens, Madeleine Philips, Lauren Kleinhans, Carmen C. Friedrich, Sven O. Martinson, Neil Venter, Amour van der Merwe, Lize Diacon, Andreas H. |
| description | Mycobacterial sputum culture is a key diagnostic and research tool.
To compare mycobacterial culture outcomes of three sputum collection methods.
We compared culture results within sets of three sputum samples collected from 18 HIV-infected adult tuberculosis patients at regular intervals up to 84 days after treatment initiation. The first sputum was collected at home and brought to the clinic, where a second and third sputum were consecutively collected under supervision following mouthwash with bottled water and chlorhexidine solution respectively. All sputa were processed for liquid culture in duplicate.
Out of 556 cultures 430 (77.3%), 91 (16.4%) and 35 (6.3%) were positive, negative or contaminated, respectively. The odds of contamination were higher with home collection and with water rinse than with chlorhexidine rinse (OR: 12.5, p |
| doi_str_mv | 10.1016/j.tube.2019.03.008 |
| format | Article |
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To compare mycobacterial culture outcomes of three sputum collection methods.
We compared culture results within sets of three sputum samples collected from 18 HIV-infected adult tuberculosis patients at regular intervals up to 84 days after treatment initiation. The first sputum was collected at home and brought to the clinic, where a second and third sputum were consecutively collected under supervision following mouthwash with bottled water and chlorhexidine solution respectively. All sputa were processed for liquid culture in duplicate.
Out of 556 cultures 430 (77.3%), 91 (16.4%) and 35 (6.3%) were positive, negative or contaminated, respectively. The odds of contamination were higher with home collection and with water rinse than with chlorhexidine rinse (OR: 12.5, p < 0.001 and OR: 6.7, p = 0.015). Chlorhexidine rinse increased the odds of a negative culture compared to water rinse (OR: 3.5, p = 0.002). The odds of a positive culture were greater with water rinse than with home collection (OR: 2.5, p = 0.005). Water rinse significantly reduced time to culture positivity.
Compared to sputum collected at home, chlorhexidine rinse reduces culture contamination and water rinse increases the rate and viable mycobacterial load of positive cultures.</description><identifier>ISSN: 1472-9792</identifier><identifier>EISSN: 1873-281X</identifier><identifier>DOI: 10.1016/j.tube.2019.03.008</identifier><identifier>PMID: 31153513</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Adult ; Antitubercular Agents - therapeutic use ; Bacteriological Techniques ; Bottled water ; Chlorhexidine ; Chlorhexidine - administration & dosage ; Coinfection ; Collection ; Contamination ; Culture ; Diagnostic software ; Diagnostic systems ; Drinking Water ; Female ; HIV ; HIV Infections - diagnosis ; HIV-Infection ; Human immunodeficiency virus ; Humans ; Liquid culture ; Load distribution ; Male ; Microbial Viability ; Mouth wash ; Mouthwashes ; Mouthwashes - administration & dosage ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - growth & development ; Mycobacterium tuberculosis - isolation & purification ; Predictive Value of Tests ; Reproducibility of Results ; Specimen Handling - methods ; Sputum ; Sputum - microbiology ; Sputum collection ; Time Factors ; Treatment Outcome ; Tuberculosis ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - drug therapy ; Tuberculosis, Pulmonary - microbiology ; Water pollution</subject><ispartof>Tuberculosis (Edinburgh, Scotland), 2019-05, Vol.116, p.17-21</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. May 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-5b4d25feaa77483deb98e30208166d1a34740760af58635644774ca5e99939ce3</citedby><cites>FETCH-LOGICAL-c384t-5b4d25feaa77483deb98e30208166d1a34740760af58635644774ca5e99939ce3</cites><orcidid>0000-0001-9705-8126</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1472979218304669$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31153513$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lourens, Madeleine</creatorcontrib><creatorcontrib>Philips, Lauren</creatorcontrib><creatorcontrib>Kleinhans, Carmen C.</creatorcontrib><creatorcontrib>Friedrich, Sven O.</creatorcontrib><creatorcontrib>Martinson, Neil</creatorcontrib><creatorcontrib>Venter, Amour</creatorcontrib><creatorcontrib>van der Merwe, Lize</creatorcontrib><creatorcontrib>Diacon, Andreas H.</creatorcontrib><title>Liquid mycobacterial culture outcomes after different sputum collection techniques before and during treatment</title><title>Tuberculosis (Edinburgh, Scotland)</title><addtitle>Tuberculosis (Edinb)</addtitle><description>Mycobacterial sputum culture is a key diagnostic and research tool.
To compare mycobacterial culture outcomes of three sputum collection methods.
We compared culture results within sets of three sputum samples collected from 18 HIV-infected adult tuberculosis patients at regular intervals up to 84 days after treatment initiation. The first sputum was collected at home and brought to the clinic, where a second and third sputum were consecutively collected under supervision following mouthwash with bottled water and chlorhexidine solution respectively. All sputa were processed for liquid culture in duplicate.
Out of 556 cultures 430 (77.3%), 91 (16.4%) and 35 (6.3%) were positive, negative or contaminated, respectively. The odds of contamination were higher with home collection and with water rinse than with chlorhexidine rinse (OR: 12.5, p < 0.001 and OR: 6.7, p = 0.015). Chlorhexidine rinse increased the odds of a negative culture compared to water rinse (OR: 3.5, p = 0.002). The odds of a positive culture were greater with water rinse than with home collection (OR: 2.5, p = 0.005). Water rinse significantly reduced time to culture positivity.
Compared to sputum collected at home, chlorhexidine rinse reduces culture contamination and water rinse increases the rate and viable mycobacterial load of positive cultures.</description><subject>Adult</subject><subject>Antitubercular Agents - therapeutic use</subject><subject>Bacteriological Techniques</subject><subject>Bottled water</subject><subject>Chlorhexidine</subject><subject>Chlorhexidine - administration & dosage</subject><subject>Coinfection</subject><subject>Collection</subject><subject>Contamination</subject><subject>Culture</subject><subject>Diagnostic software</subject><subject>Diagnostic systems</subject><subject>Drinking Water</subject><subject>Female</subject><subject>HIV</subject><subject>HIV Infections - diagnosis</subject><subject>HIV-Infection</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Liquid culture</subject><subject>Load distribution</subject><subject>Male</subject><subject>Microbial Viability</subject><subject>Mouth wash</subject><subject>Mouthwashes</subject><subject>Mouthwashes - administration & dosage</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - growth & development</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Predictive Value of Tests</subject><subject>Reproducibility of Results</subject><subject>Specimen Handling - methods</subject><subject>Sputum</subject><subject>Sputum - microbiology</subject><subject>Sputum collection</subject><subject>Time Factors</subject><subject>Treatment Outcome</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><subject>Tuberculosis, Pulmonary - drug therapy</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><subject>Water pollution</subject><issn>1472-9792</issn><issn>1873-281X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuLFTEQhYMozjj6B1xIwI2bbvPsTsCNDL7gghsFdyGdVGsu3ck1D2H-vbnc0YULV1VQ3zkU5yD0nJKREjq9Po61LTAyQvVI-EiIeoCuqZr5wBT99rDvYmaDnjW7Qk9KOZIuIoo8RlecUskl5dcoHsLPFjze71xarKuQg92wa1ttGXBq1aUdCrZrv2Af1hUyxIrLqdW2Y5e2DVwNKeIK7kfsXh1eYE1dbKPHvuUQv-Oawda9C5-iR6vdCjy7nzfo6_t3X24_DofPHz7dvj0MjitRB7kIz-QK1s6zUNzDohVwwoii0-Sp5WIWZJ6IXaWauJyE6JyzErTWXDvgN-jVxfeU0_mnavZQHGybjZBaMYxxIXpSWnb05T_oMbUc-3edEhOTigvaKXahXE6lZFjNKYfd5jtDiTm3YY7m3IY5t2EIN72NLnpxb92WHfxfyZ_4O_DmAkDP4leAbIoLEB34kHuuxqfwP__fb3KceQ</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Lourens, Madeleine</creator><creator>Philips, Lauren</creator><creator>Kleinhans, Carmen C.</creator><creator>Friedrich, Sven O.</creator><creator>Martinson, Neil</creator><creator>Venter, Amour</creator><creator>van der Merwe, Lize</creator><creator>Diacon, Andreas H.</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9705-8126</orcidid></search><sort><creationdate>201905</creationdate><title>Liquid mycobacterial culture outcomes after different sputum collection techniques before and during treatment</title><author>Lourens, Madeleine ; Philips, Lauren ; Kleinhans, Carmen C. ; Friedrich, Sven O. ; Martinson, Neil ; Venter, Amour ; van der Merwe, Lize ; Diacon, Andreas H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-5b4d25feaa77483deb98e30208166d1a34740760af58635644774ca5e99939ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Antitubercular Agents - therapeutic use</topic><topic>Bacteriological Techniques</topic><topic>Bottled water</topic><topic>Chlorhexidine</topic><topic>Chlorhexidine - administration & dosage</topic><topic>Coinfection</topic><topic>Collection</topic><topic>Contamination</topic><topic>Culture</topic><topic>Diagnostic software</topic><topic>Diagnostic systems</topic><topic>Drinking Water</topic><topic>Female</topic><topic>HIV</topic><topic>HIV Infections - diagnosis</topic><topic>HIV-Infection</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Liquid culture</topic><topic>Load distribution</topic><topic>Male</topic><topic>Microbial Viability</topic><topic>Mouth wash</topic><topic>Mouthwashes</topic><topic>Mouthwashes - administration & dosage</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Mycobacterium tuberculosis - growth & development</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Predictive Value of Tests</topic><topic>Reproducibility of Results</topic><topic>Specimen Handling - methods</topic><topic>Sputum</topic><topic>Sputum - microbiology</topic><topic>Sputum collection</topic><topic>Time Factors</topic><topic>Treatment Outcome</topic><topic>Tuberculosis</topic><topic>Tuberculosis, Pulmonary - diagnosis</topic><topic>Tuberculosis, Pulmonary - drug therapy</topic><topic>Tuberculosis, Pulmonary - microbiology</topic><topic>Water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lourens, Madeleine</creatorcontrib><creatorcontrib>Philips, Lauren</creatorcontrib><creatorcontrib>Kleinhans, Carmen C.</creatorcontrib><creatorcontrib>Friedrich, Sven O.</creatorcontrib><creatorcontrib>Martinson, Neil</creatorcontrib><creatorcontrib>Venter, Amour</creatorcontrib><creatorcontrib>van der Merwe, Lize</creatorcontrib><creatorcontrib>Diacon, Andreas H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lourens, Madeleine</au><au>Philips, Lauren</au><au>Kleinhans, Carmen C.</au><au>Friedrich, Sven O.</au><au>Martinson, Neil</au><au>Venter, Amour</au><au>van der Merwe, Lize</au><au>Diacon, Andreas H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid mycobacterial culture outcomes after different sputum collection techniques before and during treatment</atitle><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle><addtitle>Tuberculosis (Edinb)</addtitle><date>2019-05</date><risdate>2019</risdate><volume>116</volume><spage>17</spage><epage>21</epage><pages>17-21</pages><issn>1472-9792</issn><eissn>1873-281X</eissn><abstract>Mycobacterial sputum culture is a key diagnostic and research tool.
To compare mycobacterial culture outcomes of three sputum collection methods.
We compared culture results within sets of three sputum samples collected from 18 HIV-infected adult tuberculosis patients at regular intervals up to 84 days after treatment initiation. The first sputum was collected at home and brought to the clinic, where a second and third sputum were consecutively collected under supervision following mouthwash with bottled water and chlorhexidine solution respectively. All sputa were processed for liquid culture in duplicate.
Out of 556 cultures 430 (77.3%), 91 (16.4%) and 35 (6.3%) were positive, negative or contaminated, respectively. The odds of contamination were higher with home collection and with water rinse than with chlorhexidine rinse (OR: 12.5, p < 0.001 and OR: 6.7, p = 0.015). Chlorhexidine rinse increased the odds of a negative culture compared to water rinse (OR: 3.5, p = 0.002). The odds of a positive culture were greater with water rinse than with home collection (OR: 2.5, p = 0.005). Water rinse significantly reduced time to culture positivity.
Compared to sputum collected at home, chlorhexidine rinse reduces culture contamination and water rinse increases the rate and viable mycobacterial load of positive cultures.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>31153513</pmid><doi>10.1016/j.tube.2019.03.008</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-9705-8126</orcidid></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Antitubercular Agents - therapeutic use Bacteriological Techniques Bottled water Chlorhexidine Chlorhexidine - administration & dosage Coinfection Collection Contamination Culture Diagnostic software Diagnostic systems Drinking Water Female HIV HIV Infections - diagnosis HIV-Infection Human immunodeficiency virus Humans Liquid culture Load distribution Male Microbial Viability Mouth wash Mouthwashes Mouthwashes - administration & dosage Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - isolation & purification Predictive Value of Tests Reproducibility of Results Specimen Handling - methods Sputum Sputum - microbiology Sputum collection Time Factors Treatment Outcome Tuberculosis Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - drug therapy Tuberculosis, Pulmonary - microbiology Water pollution |
| title | Liquid mycobacterial culture outcomes after different sputum collection techniques before and during treatment |
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