Hsa_circ_0005519 increases IL‐13/IL‐6 by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma
Background Circular RNAs (circRNAs) are a class of non‐coding RNAs that could serve as novel biomarkers for the diagnosis and treatment of diseases. We hypothesized that circRNAs of CD4+ T cells are involved in asthma. Objective In this study, we investigated the circRNA expression profile and the p...
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| Veröffentlicht in: | Clinical and experimental allergy 2019-08, Vol.49 (8), p.1116-1127 |
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| container_title | Clinical and experimental allergy |
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| creator | Huang, Zhenli Cao, Yong Zhou, Min Qi, Xuefei Fu, Bohua Mou, Yong Wu, Guorao Xie, Jungang Zhao, Jianping Xiong, Weining |
| description | Background
Circular RNAs (circRNAs) are a class of non‐coding RNAs that could serve as novel biomarkers for the diagnosis and treatment of diseases. We hypothesized that circRNAs of CD4+ T cells are involved in asthma.
Objective
In this study, we investigated the circRNA expression profile and the possible mechanism by which hsa_circ_0005519 participates in asthma.
Methods
The expression profiles of circRNAs in CD4+ T cells were revealed by circRNA microarray. Hsa_circ_0005519 expression in CD4+ T cells was confirmed in asthmatic patients (n = 65) and healthy subjects (n = 30). Hsa‐let‐7a‐5p, the target of hsa_circ_0005519, was predicted by online algorithms and verified by a dual‐luciferase reporter assay. Correlation assays between the expression of hsa_circ_0005519 and hsa‐let‐7a‐5p, the mRNA levels of interleukin (IL)‐13 and IL‐6 in CD4+ T cells, and the clinical characteristics of asthmatic patients were performed. The role of hsa_circ_0005519 in proinflammatory cytokine expression was investigated in CD4+ T cells from asthmatic patients in vitro. Hsa_circ_0005519 expression in PBMCs was determined in another cohort including 30 asthmatic patients and 24 controls. Correlation assays of hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were performed.
Results
Hsa_circ_0005519 was up‐regulated and negatively correlated with hsa‐let‐7a‐5p expression in CD4+ T cells of asthmatic patients. Both the fraction of exhaled nitric oxide (FeNO) and the peripheral blood eosinophil ratio were positively correlated with hsa_circ_0005519 expression in CD4+ T cells. These outcomes were also different in asthmatic patients with low vs high hsa_circ_0005519 levels. Hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were concordant in asthmatic patients. Mechanistically, hsa_circ_0005519 might bind to hsa‐let‐7a‐5p and relieve suppression for IL‐13/IL‐6 in CD4+ T cells.
Conclusions and clinical relevance
Our data suggest that hsa_circ_0005519 may induce IL‐13 and IL‐6 expression by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma. And hsa_circ_0005519 may be a potential biomarker of asthma. |
| doi_str_mv | 10.1111/cea.13445 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2233862127</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2233862127</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3935-b294ad524ca1fed0686c59512be83ff7745e78fa30b5f89571bd2a4ce425d3d23</originalsourceid><addsrcrecordid>eNp1kc1KAzEUhYMoWqsLX0ACbhSZNr8zybLUXyi40XXIZG50ZNqpyQzSnY_gM_okplZdCN5F7iX57uGQg9ARJSOaauzAjigXQm6hAeW5zFiqbTQgWoqsUFrsof0YnwkhXGq1i_Y4pUIxTQaouYnWuDo4k16lpBrXCxfARoj4dvbx9k75-KvnuFzhAI99Y7t68Yifok23DXTpLNajXKZVPL0Q5_geO2iaiLsWW-_BddjG7mluD9COt02Ew-8-RA9Xl_fTm2x2d307ncwyxzWXWcm0sJVkwlnqoSK5yp3UkrISFPe-KISEQnnLSSm90rKgZcWscCCYrHjF-BCdbnSXoX3pIXZmXse1JbuAto-GMc5VzigrEnryB31u-7BI7hKVKyYLRniizjaUC22MAbxZhnpuw8pQYtYRmBSB-Yogscffin05h-qX_PnzBIw3wGvdwOp_JTO9nGwkPwGt4ZAE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2268257203</pqid></control><display><type>article</type><title>Hsa_circ_0005519 increases IL‐13/IL‐6 by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma</title><source>Wiley Online Library All Journals</source><creator>Huang, Zhenli ; Cao, Yong ; Zhou, Min ; Qi, Xuefei ; Fu, Bohua ; Mou, Yong ; Wu, Guorao ; Xie, Jungang ; Zhao, Jianping ; Xiong, Weining</creator><creatorcontrib>Huang, Zhenli ; Cao, Yong ; Zhou, Min ; Qi, Xuefei ; Fu, Bohua ; Mou, Yong ; Wu, Guorao ; Xie, Jungang ; Zhao, Jianping ; Xiong, Weining</creatorcontrib><description>Background
Circular RNAs (circRNAs) are a class of non‐coding RNAs that could serve as novel biomarkers for the diagnosis and treatment of diseases. We hypothesized that circRNAs of CD4+ T cells are involved in asthma.
Objective
In this study, we investigated the circRNA expression profile and the possible mechanism by which hsa_circ_0005519 participates in asthma.
Methods
The expression profiles of circRNAs in CD4+ T cells were revealed by circRNA microarray. Hsa_circ_0005519 expression in CD4+ T cells was confirmed in asthmatic patients (n = 65) and healthy subjects (n = 30). Hsa‐let‐7a‐5p, the target of hsa_circ_0005519, was predicted by online algorithms and verified by a dual‐luciferase reporter assay. Correlation assays between the expression of hsa_circ_0005519 and hsa‐let‐7a‐5p, the mRNA levels of interleukin (IL)‐13 and IL‐6 in CD4+ T cells, and the clinical characteristics of asthmatic patients were performed. The role of hsa_circ_0005519 in proinflammatory cytokine expression was investigated in CD4+ T cells from asthmatic patients in vitro. Hsa_circ_0005519 expression in PBMCs was determined in another cohort including 30 asthmatic patients and 24 controls. Correlation assays of hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were performed.
Results
Hsa_circ_0005519 was up‐regulated and negatively correlated with hsa‐let‐7a‐5p expression in CD4+ T cells of asthmatic patients. Both the fraction of exhaled nitric oxide (FeNO) and the peripheral blood eosinophil ratio were positively correlated with hsa_circ_0005519 expression in CD4+ T cells. These outcomes were also different in asthmatic patients with low vs high hsa_circ_0005519 levels. Hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were concordant in asthmatic patients. Mechanistically, hsa_circ_0005519 might bind to hsa‐let‐7a‐5p and relieve suppression for IL‐13/IL‐6 in CD4+ T cells.
Conclusions and clinical relevance
Our data suggest that hsa_circ_0005519 may induce IL‐13 and IL‐6 expression by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma. And hsa_circ_0005519 may be a potential biomarker of asthma.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/cea.13445</identifier><identifier>PMID: 31148290</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Assaying ; Asthma ; Biomarkers ; CD4 antigen ; CD4+ T cells ; hsa_circ_0005519 ; hsa‐let‐7a‐5p ; Inflammation ; Leukocytes (eosinophilic) ; Lymphocytes ; Lymphocytes T ; Medical treatment ; mRNA ; Nitric oxide ; Patients ; PBMCs ; Peripheral blood</subject><ispartof>Clinical and experimental allergy, 2019-08, Vol.49 (8), p.1116-1127</ispartof><rights>2019 John Wiley & Sons Ltd</rights><rights>2019 John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3935-b294ad524ca1fed0686c59512be83ff7745e78fa30b5f89571bd2a4ce425d3d23</citedby><cites>FETCH-LOGICAL-c3935-b294ad524ca1fed0686c59512be83ff7745e78fa30b5f89571bd2a4ce425d3d23</cites><orcidid>0000-0002-2486-6517</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fcea.13445$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fcea.13445$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31148290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Zhenli</creatorcontrib><creatorcontrib>Cao, Yong</creatorcontrib><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Qi, Xuefei</creatorcontrib><creatorcontrib>Fu, Bohua</creatorcontrib><creatorcontrib>Mou, Yong</creatorcontrib><creatorcontrib>Wu, Guorao</creatorcontrib><creatorcontrib>Xie, Jungang</creatorcontrib><creatorcontrib>Zhao, Jianping</creatorcontrib><creatorcontrib>Xiong, Weining</creatorcontrib><title>Hsa_circ_0005519 increases IL‐13/IL‐6 by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Background
Circular RNAs (circRNAs) are a class of non‐coding RNAs that could serve as novel biomarkers for the diagnosis and treatment of diseases. We hypothesized that circRNAs of CD4+ T cells are involved in asthma.
Objective
In this study, we investigated the circRNA expression profile and the possible mechanism by which hsa_circ_0005519 participates in asthma.
Methods
The expression profiles of circRNAs in CD4+ T cells were revealed by circRNA microarray. Hsa_circ_0005519 expression in CD4+ T cells was confirmed in asthmatic patients (n = 65) and healthy subjects (n = 30). Hsa‐let‐7a‐5p, the target of hsa_circ_0005519, was predicted by online algorithms and verified by a dual‐luciferase reporter assay. Correlation assays between the expression of hsa_circ_0005519 and hsa‐let‐7a‐5p, the mRNA levels of interleukin (IL)‐13 and IL‐6 in CD4+ T cells, and the clinical characteristics of asthmatic patients were performed. The role of hsa_circ_0005519 in proinflammatory cytokine expression was investigated in CD4+ T cells from asthmatic patients in vitro. Hsa_circ_0005519 expression in PBMCs was determined in another cohort including 30 asthmatic patients and 24 controls. Correlation assays of hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were performed.
Results
Hsa_circ_0005519 was up‐regulated and negatively correlated with hsa‐let‐7a‐5p expression in CD4+ T cells of asthmatic patients. Both the fraction of exhaled nitric oxide (FeNO) and the peripheral blood eosinophil ratio were positively correlated with hsa_circ_0005519 expression in CD4+ T cells. These outcomes were also different in asthmatic patients with low vs high hsa_circ_0005519 levels. Hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were concordant in asthmatic patients. Mechanistically, hsa_circ_0005519 might bind to hsa‐let‐7a‐5p and relieve suppression for IL‐13/IL‐6 in CD4+ T cells.
Conclusions and clinical relevance
Our data suggest that hsa_circ_0005519 may induce IL‐13 and IL‐6 expression by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma. And hsa_circ_0005519 may be a potential biomarker of asthma.</description><subject>Assaying</subject><subject>Asthma</subject><subject>Biomarkers</subject><subject>CD4 antigen</subject><subject>CD4+ T cells</subject><subject>hsa_circ_0005519</subject><subject>hsa‐let‐7a‐5p</subject><subject>Inflammation</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Medical treatment</subject><subject>mRNA</subject><subject>Nitric oxide</subject><subject>Patients</subject><subject>PBMCs</subject><subject>Peripheral blood</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kc1KAzEUhYMoWqsLX0ACbhSZNr8zybLUXyi40XXIZG50ZNqpyQzSnY_gM_okplZdCN5F7iX57uGQg9ARJSOaauzAjigXQm6hAeW5zFiqbTQgWoqsUFrsof0YnwkhXGq1i_Y4pUIxTQaouYnWuDo4k16lpBrXCxfARoj4dvbx9k75-KvnuFzhAI99Y7t68Yifok23DXTpLNajXKZVPL0Q5_geO2iaiLsWW-_BddjG7mluD9COt02Ew-8-RA9Xl_fTm2x2d307ncwyxzWXWcm0sJVkwlnqoSK5yp3UkrISFPe-KISEQnnLSSm90rKgZcWscCCYrHjF-BCdbnSXoX3pIXZmXse1JbuAto-GMc5VzigrEnryB31u-7BI7hKVKyYLRniizjaUC22MAbxZhnpuw8pQYtYRmBSB-Yogscffin05h-qX_PnzBIw3wGvdwOp_JTO9nGwkPwGt4ZAE</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Huang, Zhenli</creator><creator>Cao, Yong</creator><creator>Zhou, Min</creator><creator>Qi, Xuefei</creator><creator>Fu, Bohua</creator><creator>Mou, Yong</creator><creator>Wu, Guorao</creator><creator>Xie, Jungang</creator><creator>Zhao, Jianping</creator><creator>Xiong, Weining</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2486-6517</orcidid></search><sort><creationdate>201908</creationdate><title>Hsa_circ_0005519 increases IL‐13/IL‐6 by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma</title><author>Huang, Zhenli ; Cao, Yong ; Zhou, Min ; Qi, Xuefei ; Fu, Bohua ; Mou, Yong ; Wu, Guorao ; Xie, Jungang ; Zhao, Jianping ; Xiong, Weining</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3935-b294ad524ca1fed0686c59512be83ff7745e78fa30b5f89571bd2a4ce425d3d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Assaying</topic><topic>Asthma</topic><topic>Biomarkers</topic><topic>CD4 antigen</topic><topic>CD4+ T cells</topic><topic>hsa_circ_0005519</topic><topic>hsa‐let‐7a‐5p</topic><topic>Inflammation</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Medical treatment</topic><topic>mRNA</topic><topic>Nitric oxide</topic><topic>Patients</topic><topic>PBMCs</topic><topic>Peripheral blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Zhenli</creatorcontrib><creatorcontrib>Cao, Yong</creatorcontrib><creatorcontrib>Zhou, Min</creatorcontrib><creatorcontrib>Qi, Xuefei</creatorcontrib><creatorcontrib>Fu, Bohua</creatorcontrib><creatorcontrib>Mou, Yong</creatorcontrib><creatorcontrib>Wu, Guorao</creatorcontrib><creatorcontrib>Xie, Jungang</creatorcontrib><creatorcontrib>Zhao, Jianping</creatorcontrib><creatorcontrib>Xiong, Weining</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Zhenli</au><au>Cao, Yong</au><au>Zhou, Min</au><au>Qi, Xuefei</au><au>Fu, Bohua</au><au>Mou, Yong</au><au>Wu, Guorao</au><au>Xie, Jungang</au><au>Zhao, Jianping</au><au>Xiong, Weining</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hsa_circ_0005519 increases IL‐13/IL‐6 by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2019-08</date><risdate>2019</risdate><volume>49</volume><issue>8</issue><spage>1116</spage><epage>1127</epage><pages>1116-1127</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Background
Circular RNAs (circRNAs) are a class of non‐coding RNAs that could serve as novel biomarkers for the diagnosis and treatment of diseases. We hypothesized that circRNAs of CD4+ T cells are involved in asthma.
Objective
In this study, we investigated the circRNA expression profile and the possible mechanism by which hsa_circ_0005519 participates in asthma.
Methods
The expression profiles of circRNAs in CD4+ T cells were revealed by circRNA microarray. Hsa_circ_0005519 expression in CD4+ T cells was confirmed in asthmatic patients (n = 65) and healthy subjects (n = 30). Hsa‐let‐7a‐5p, the target of hsa_circ_0005519, was predicted by online algorithms and verified by a dual‐luciferase reporter assay. Correlation assays between the expression of hsa_circ_0005519 and hsa‐let‐7a‐5p, the mRNA levels of interleukin (IL)‐13 and IL‐6 in CD4+ T cells, and the clinical characteristics of asthmatic patients were performed. The role of hsa_circ_0005519 in proinflammatory cytokine expression was investigated in CD4+ T cells from asthmatic patients in vitro. Hsa_circ_0005519 expression in PBMCs was determined in another cohort including 30 asthmatic patients and 24 controls. Correlation assays of hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were performed.
Results
Hsa_circ_0005519 was up‐regulated and negatively correlated with hsa‐let‐7a‐5p expression in CD4+ T cells of asthmatic patients. Both the fraction of exhaled nitric oxide (FeNO) and the peripheral blood eosinophil ratio were positively correlated with hsa_circ_0005519 expression in CD4+ T cells. These outcomes were also different in asthmatic patients with low vs high hsa_circ_0005519 levels. Hsa_circ_0005519 expressions between CD4+ T cells and PBMCs were concordant in asthmatic patients. Mechanistically, hsa_circ_0005519 might bind to hsa‐let‐7a‐5p and relieve suppression for IL‐13/IL‐6 in CD4+ T cells.
Conclusions and clinical relevance
Our data suggest that hsa_circ_0005519 may induce IL‐13 and IL‐6 expression by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma. And hsa_circ_0005519 may be a potential biomarker of asthma.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31148290</pmid><doi>10.1111/cea.13445</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-2486-6517</orcidid></addata></record> |
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| ispartof | Clinical and experimental allergy, 2019-08, Vol.49 (8), p.1116-1127 |
| issn | 0954-7894 1365-2222 |
| language | eng |
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| source | Wiley Online Library All Journals |
| subjects | Assaying Asthma Biomarkers CD4 antigen CD4+ T cells hsa_circ_0005519 hsa‐let‐7a‐5p Inflammation Leukocytes (eosinophilic) Lymphocytes Lymphocytes T Medical treatment mRNA Nitric oxide Patients PBMCs Peripheral blood |
| title | Hsa_circ_0005519 increases IL‐13/IL‐6 by regulating hsa‐let‐7a‐5p in CD4+ T cells to affect asthma |
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