Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis
The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against Leishmania infantum exo-antigens for diagnosis of VL, calle...
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creator | Saliba, Juliana W. Lopes, Karine F. Silva-Pereira, Rosiane A. Teixeira, Luciana A. S. Oliveira, Edward |
description | The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against
Leishmania infantum
exo-antigens for diagnosis of VL, called ELISA-Exo. This assay was applied in 309 masked serum samples from VL, tegumentary leishmaniasis, Chagas disease, schistosomiasis mansoni, malaria patients, and healthy individuals. The results were compared with those from ELISA using rK39 as antigen (ELISA-rK39). The ELISA assays presented sensitivity of 96.8% and 98.4% (
p
= 0.68), specificity of 92.4% for both, and diagnostic accuracy of 94.2% and 94.8% (
p
= 0.48) by the ELISA-Exo and ELISA-rK39, respectively. An excellent agreement beyond chance (Kappa index = 0.82) was obtained when the results from ELISA assays were cross-tabulated. The Western blotting showed that false-positive results presented by ELISA-Exo probably were produced by cross-reactivity of antigens shared with the species of the family
Trypanosomatidae.
In the future, an immunoproteomic approach can contribute for identification of main immunoreactive
L. infantum
exo-antigens. |
doi_str_mv | 10.1007/s00436-019-06352-4 |
format | Article |
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Leishmania infantum
exo-antigens for diagnosis of VL, called ELISA-Exo. This assay was applied in 309 masked serum samples from VL, tegumentary leishmaniasis, Chagas disease, schistosomiasis mansoni, malaria patients, and healthy individuals. The results were compared with those from ELISA using rK39 as antigen (ELISA-rK39). The ELISA assays presented sensitivity of 96.8% and 98.4% (
p
= 0.68), specificity of 92.4% for both, and diagnostic accuracy of 94.2% and 94.8% (
p
= 0.48) by the ELISA-Exo and ELISA-rK39, respectively. An excellent agreement beyond chance (Kappa index = 0.82) was obtained when the results from ELISA assays were cross-tabulated. The Western blotting showed that false-positive results presented by ELISA-Exo probably were produced by cross-reactivity of antigens shared with the species of the family
Trypanosomatidae.
In the future, an immunoproteomic approach can contribute for identification of main immunoreactive
L. infantum
exo-antigens.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-019-06352-4</identifier><identifier>PMID: 31144033</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antigens ; Biomedical and Life Sciences ; Biomedicine ; Chagas disease ; Cross-reactivity ; Diagnosis ; Enzyme-linked immunosorbent assay ; Enzymes ; Immunoglobulin G ; Immunology ; Kala-azar ; Leishmania infantum ; Malaria ; Medical Microbiology ; Microbiology ; Parasitic diseases ; Protozoology - Short Communication ; Schistosomiasis ; Tegumentary leishmaniasis ; Tropical diseases ; Visceral leishmaniasis ; Western blotting</subject><ispartof>Parasitology research (1987), 2019-07, Vol.118 (7), p.2317-2323</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-826cf83a236bd79f722f2129c1c6e9c2d3dfc5c74704421f12bacd054c66e9e53</citedby><cites>FETCH-LOGICAL-c442t-826cf83a236bd79f722f2129c1c6e9c2d3dfc5c74704421f12bacd054c66e9e53</cites><orcidid>0000-0002-0042-5178</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-019-06352-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-019-06352-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31144033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saliba, Juliana W.</creatorcontrib><creatorcontrib>Lopes, Karine F.</creatorcontrib><creatorcontrib>Silva-Pereira, Rosiane A.</creatorcontrib><creatorcontrib>Teixeira, Luciana A. S.</creatorcontrib><creatorcontrib>Oliveira, Edward</creatorcontrib><title>Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against
Leishmania infantum
exo-antigens for diagnosis of VL, called ELISA-Exo. This assay was applied in 309 masked serum samples from VL, tegumentary leishmaniasis, Chagas disease, schistosomiasis mansoni, malaria patients, and healthy individuals. The results were compared with those from ELISA using rK39 as antigen (ELISA-rK39). The ELISA assays presented sensitivity of 96.8% and 98.4% (
p
= 0.68), specificity of 92.4% for both, and diagnostic accuracy of 94.2% and 94.8% (
p
= 0.48) by the ELISA-Exo and ELISA-rK39, respectively. An excellent agreement beyond chance (Kappa index = 0.82) was obtained when the results from ELISA assays were cross-tabulated. The Western blotting showed that false-positive results presented by ELISA-Exo probably were produced by cross-reactivity of antigens shared with the species of the family
Trypanosomatidae.
In the future, an immunoproteomic approach can contribute for identification of main immunoreactive
L. infantum
exo-antigens.</description><subject>Antigens</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Chagas disease</subject><subject>Cross-reactivity</subject><subject>Diagnosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Immunoglobulin G</subject><subject>Immunology</subject><subject>Kala-azar</subject><subject>Leishmania infantum</subject><subject>Malaria</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Parasitic diseases</subject><subject>Protozoology - Short Communication</subject><subject>Schistosomiasis</subject><subject>Tegumentary leishmaniasis</subject><subject>Tropical diseases</subject><subject>Visceral leishmaniasis</subject><subject>Western blotting</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU9P3DAQxa2qqCxLv0APKFIvXLJ4bMfZcEMIaKWVuMDZ8vpP6lViL3ZS4NvjsEBbhCofPJr5zdOzH0LfAC8A4_okYcwoLzE0Jea0IiX7hGbAKCmhqarPaIabXGMAuo8OUtpgDDVn7AvapwCMYUpnSK-MS7966Z0snLfSD2NfmIdQ5sq1xqfTQm63nVNycMEXQ7iXURfJxNCFNne7QjvZ-pBcKoItfrukTMzd7k02Tw7RnpVdMl9f7jm6vby4Of9Rrq6vfp6frUrFGBnKJeHKLqkklK913diaEEuANAoUN40immqrKlWzGmceLJC1VBpXTPE8NxWdo-Od7jaGu9GkQfSTn66T3oQxCUIoYTVr-IR-f4duwhh9dpepivAlwN9UKzsj8v-EIUo1iYqzqsk--RTBHC0-oPLRpncqeGNd7v-zQHYLKoaUorFiG10v46MALKZoxS5akaMVz9GKaenoxfG47o1-W3nNMgN0B6Q88q2Jf570H9knJtGt3w</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Saliba, Juliana W.</creator><creator>Lopes, Karine F.</creator><creator>Silva-Pereira, Rosiane A.</creator><creator>Teixeira, Luciana A. S.</creator><creator>Oliveira, Edward</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0042-5178</orcidid></search><sort><creationdate>20190701</creationdate><title>Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis</title><author>Saliba, Juliana W. ; Lopes, Karine F. ; Silva-Pereira, Rosiane A. ; Teixeira, Luciana A. S. ; Oliveira, Edward</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-826cf83a236bd79f722f2129c1c6e9c2d3dfc5c74704421f12bacd054c66e9e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antigens</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Chagas disease</topic><topic>Cross-reactivity</topic><topic>Diagnosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Immunoglobulin G</topic><topic>Immunology</topic><topic>Kala-azar</topic><topic>Leishmania infantum</topic><topic>Malaria</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Parasitic diseases</topic><topic>Protozoology - Short Communication</topic><topic>Schistosomiasis</topic><topic>Tegumentary leishmaniasis</topic><topic>Tropical diseases</topic><topic>Visceral leishmaniasis</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saliba, Juliana W.</creatorcontrib><creatorcontrib>Lopes, Karine F.</creatorcontrib><creatorcontrib>Silva-Pereira, Rosiane A.</creatorcontrib><creatorcontrib>Teixeira, Luciana A. S.</creatorcontrib><creatorcontrib>Oliveira, Edward</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saliba, Juliana W.</au><au>Lopes, Karine F.</au><au>Silva-Pereira, Rosiane A.</au><au>Teixeira, Luciana A. S.</au><au>Oliveira, Edward</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>118</volume><issue>7</issue><spage>2317</spage><epage>2323</epage><pages>2317-2323</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against
Leishmania infantum
exo-antigens for diagnosis of VL, called ELISA-Exo. This assay was applied in 309 masked serum samples from VL, tegumentary leishmaniasis, Chagas disease, schistosomiasis mansoni, malaria patients, and healthy individuals. The results were compared with those from ELISA using rK39 as antigen (ELISA-rK39). The ELISA assays presented sensitivity of 96.8% and 98.4% (
p
= 0.68), specificity of 92.4% for both, and diagnostic accuracy of 94.2% and 94.8% (
p
= 0.48) by the ELISA-Exo and ELISA-rK39, respectively. An excellent agreement beyond chance (Kappa index = 0.82) was obtained when the results from ELISA assays were cross-tabulated. The Western blotting showed that false-positive results presented by ELISA-Exo probably were produced by cross-reactivity of antigens shared with the species of the family
Trypanosomatidae.
In the future, an immunoproteomic approach can contribute for identification of main immunoreactive
L. infantum
exo-antigens.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31144033</pmid><doi>10.1007/s00436-019-06352-4</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0042-5178</orcidid></addata></record> |
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subjects | Antigens Biomedical and Life Sciences Biomedicine Chagas disease Cross-reactivity Diagnosis Enzyme-linked immunosorbent assay Enzymes Immunoglobulin G Immunology Kala-azar Leishmania infantum Malaria Medical Microbiology Microbiology Parasitic diseases Protozoology - Short Communication Schistosomiasis Tegumentary leishmaniasis Tropical diseases Visceral leishmaniasis Western blotting |
title | Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis |
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