In-Syringe Electrokinetic Ampholytes Focusing Coupled with Electrospray Ionization Mass Spectrometry

A 25 μL analytical glass syringe has been used for isoelectric focusing (IEF) utilizing the stainless-steel needle and plunger as electrodes. The generation of protons and hydroxyl ions at the electrodes facilitated a neutralization reaction boundary (NRB) mechanism to focus different amphoteric com...

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Veröffentlicht in:	Analytical chemistry (Washington) 2019-07, Vol.91 (13), p.8259-8266
Hauptverfasser:	Mikhail, Ibraam E, Tehranirokh, Masoomeh, Gooley, Andrew A, Guijt, Rosanne M, Breadmore, Michael C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitrile Analytical chemistry Bovine serum albumin Calibration Chemistry Correlation coefficient Correlation coefficients Coupling (molecular) Electrodes Electrokinetics Electrospraying Flow velocity Formic acid Hemoglobin Histidine Histidinemia Hydroxyl ions Ionization Ions Isoelectric focusing Mass spectrometry Mass spectroscopy Mathematical analysis Neutralization Optimization Process parameters Protons Scientific imaging Serum albumin Sheaths Spectroscopy Steel Urine
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