Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize
Key message Mainly additive gene action governed inheritance of haploid male fertility, although epistatic effects were also significant. Recurrent selection for haploid male fertility resulted in substantial improvement in this trait. The doubled haploid (DH) technology offers several advantages in...
Gespeichert in:
| Veröffentlicht in: | Theoretical and applied genetics 2019-08, Vol.132 (8), p.2273-2284 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2284 |
|---|---|
| container_issue | 8 |
| container_start_page | 2273 |
| container_title | Theoretical and applied genetics |
| container_volume | 132 |
| creator | Molenaar, Willem S. Schipprack, Wolfgang Brauner, Pedro C. Melchinger, Albrecht E. |
| description | Key message
Mainly additive gene action governed inheritance of haploid male fertility, although epistatic effects were also significant. Recurrent selection for haploid male fertility resulted in substantial improvement in this trait.
The doubled haploid (DH) technology offers several advantages in maize breeding compared to the traditional method of recurrent selfing. However, there is still great potential for improving the success rate of DH production. Currently, the majority of haploid plants are infertile after chromosome doubling treatment by antimitotic agents such as colchicine and cannot be selfed for production of DH lines. Improvement in haploid male fertility (HMF) by selection for a higher spontaneous chromosome doubling rate (SDR) has the potential to increase DH production efficiency. To investigate the gene action governing SDR in two breeding populations, we adapted the quantitative-genetic model of Eberhart and Gardner (in Biometrics 22:864–881.
https://doi.org/10.2307/2528079
,
1966)
for the case of haploid progeny from ten DH lines and corresponding diallel crosses. Furthermore, we carried out three cycles of recurrent selection for SDR in two additional populations to evaluate the selection gain for this trait. Additive genetic effects predominated in both diallel crosses, but epistatic effects were also significant. Entry-mean heritability of SDR observed for haploid progeny of these populations exceeded 0.91, but the single-plant heritability relevant to selection was low, ranging from 0.11 to 0.19. Recurrent selection increased SDR from approximately 5–50%, which suggests the presence of few QTL with large effects. This improvement in HMF is greater than the effect of standard colchicine treatment, which yields at maximum 30% fertile haploids. Altogether, the results show the great potential of spontaneous chromosome doubling to streamline development DH lines and to enable new breeding schemes with more efficient allocation of resources. |
| doi_str_mv | 10.1007/s00122-019-03353-w |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2232131362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A593291999</galeid><sourcerecordid>A593291999</sourcerecordid><originalsourceid>FETCH-LOGICAL-c476t-5b51e05fa7925023fd1f95e6271d1d563f1d41cbf969ac7658caaad40cefda9e3</originalsourceid><addsrcrecordid>eNp9kltrFTEUhYMo9lj9Az5IwBd9mJrLZMY8lqK2UBC8PIecZOeYkkmOSaYXwf9uTqdajojkIbDzrU3WYiH0nJIjSsj4phBCGesIlR3hXPDu6gFa0Z6zjrGePUQrQnrSiVGwA_SklAtCCBOEP0YHnJKBkV6s0M9TvQ3JWzzpANhBrj74eoN1tLhsU6w6QpoLNt9ymlJJE2Cb5nXwcYPhUodZV7DYR6yx9ToECLfSDGbOGWLFBQKY6lPEcL2F7KfdsPGT9j_gKXrkdCjw7O4-RF_fv_tyctqdf_xwdnJ83pl-HGon1oICEU6Pshlg3FnqpICBjdRSKwbuqO2pWTs5SG3GQbw1WmvbEwPOagn8EL1a9m5z-j5DqWryxUAIizvFGGeUUz6whr78C71Ic47td41iRLAW6nBPbVpsykeXatZmt1QdC8mZpFLKRh39g2rHwuRNiuB8m-8JXu8JGlPhum70XIo6-_xpn2ULa3IqJYNT25auzjeKErXrh1r6oVo_1G0_1FUTvbhzN68nsH8kvwvRAL4ApT3FDeR7-_9Z-wvhGsYa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2220520046</pqid></control><display><type>article</type><title>Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Molenaar, Willem S. ; Schipprack, Wolfgang ; Brauner, Pedro C. ; Melchinger, Albrecht E.</creator><creatorcontrib>Molenaar, Willem S. ; Schipprack, Wolfgang ; Brauner, Pedro C. ; Melchinger, Albrecht E.</creatorcontrib><description>Key message
Mainly additive gene action governed inheritance of haploid male fertility, although epistatic effects were also significant. Recurrent selection for haploid male fertility resulted in substantial improvement in this trait.
The doubled haploid (DH) technology offers several advantages in maize breeding compared to the traditional method of recurrent selfing. However, there is still great potential for improving the success rate of DH production. Currently, the majority of haploid plants are infertile after chromosome doubling treatment by antimitotic agents such as colchicine and cannot be selfed for production of DH lines. Improvement in haploid male fertility (HMF) by selection for a higher spontaneous chromosome doubling rate (SDR) has the potential to increase DH production efficiency. To investigate the gene action governing SDR in two breeding populations, we adapted the quantitative-genetic model of Eberhart and Gardner (in Biometrics 22:864–881.
https://doi.org/10.2307/2528079
,
1966)
for the case of haploid progeny from ten DH lines and corresponding diallel crosses. Furthermore, we carried out three cycles of recurrent selection for SDR in two additional populations to evaluate the selection gain for this trait. Additive genetic effects predominated in both diallel crosses, but epistatic effects were also significant. Entry-mean heritability of SDR observed for haploid progeny of these populations exceeded 0.91, but the single-plant heritability relevant to selection was low, ranging from 0.11 to 0.19. Recurrent selection increased SDR from approximately 5–50%, which suggests the presence of few QTL with large effects. This improvement in HMF is greater than the effect of standard colchicine treatment, which yields at maximum 30% fertile haploids. Altogether, the results show the great potential of spontaneous chromosome doubling to streamline development DH lines and to enable new breeding schemes with more efficient allocation of resources.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-019-03353-w</identifier><identifier>PMID: 31062045</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Antimitotic agents ; Biochemistry ; Biomedical and Life Sciences ; Biometrics ; Biotechnology ; Breeding ; Chromosomes ; Chromosomes, Plant - genetics ; Colchicine ; Corn ; Crosses, Genetic ; Epistasis ; Fertility ; Fertility - genetics ; Genetic crosses ; Genotype ; Haploidy ; Heredity ; Heritability ; Life Sciences ; Original Article ; Plant Biochemistry ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Pollen - genetics ; Quantitative genetics ; Quantitative trait loci ; Selection, Genetic ; Zea mays - genetics ; Zea mays - physiology</subject><ispartof>Theoretical and applied genetics, 2019-08, Vol.132 (8), p.2273-2284</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Theoretical and Applied Genetics is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-5b51e05fa7925023fd1f95e6271d1d563f1d41cbf969ac7658caaad40cefda9e3</citedby><cites>FETCH-LOGICAL-c476t-5b51e05fa7925023fd1f95e6271d1d563f1d41cbf969ac7658caaad40cefda9e3</cites><orcidid>0000-0003-0810-873X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00122-019-03353-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00122-019-03353-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31062045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Molenaar, Willem S.</creatorcontrib><creatorcontrib>Schipprack, Wolfgang</creatorcontrib><creatorcontrib>Brauner, Pedro C.</creatorcontrib><creatorcontrib>Melchinger, Albrecht E.</creatorcontrib><title>Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><addtitle>Theor Appl Genet</addtitle><description>Key message
Mainly additive gene action governed inheritance of haploid male fertility, although epistatic effects were also significant. Recurrent selection for haploid male fertility resulted in substantial improvement in this trait.
The doubled haploid (DH) technology offers several advantages in maize breeding compared to the traditional method of recurrent selfing. However, there is still great potential for improving the success rate of DH production. Currently, the majority of haploid plants are infertile after chromosome doubling treatment by antimitotic agents such as colchicine and cannot be selfed for production of DH lines. Improvement in haploid male fertility (HMF) by selection for a higher spontaneous chromosome doubling rate (SDR) has the potential to increase DH production efficiency. To investigate the gene action governing SDR in two breeding populations, we adapted the quantitative-genetic model of Eberhart and Gardner (in Biometrics 22:864–881.
https://doi.org/10.2307/2528079
,
1966)
for the case of haploid progeny from ten DH lines and corresponding diallel crosses. Furthermore, we carried out three cycles of recurrent selection for SDR in two additional populations to evaluate the selection gain for this trait. Additive genetic effects predominated in both diallel crosses, but epistatic effects were also significant. Entry-mean heritability of SDR observed for haploid progeny of these populations exceeded 0.91, but the single-plant heritability relevant to selection was low, ranging from 0.11 to 0.19. Recurrent selection increased SDR from approximately 5–50%, which suggests the presence of few QTL with large effects. This improvement in HMF is greater than the effect of standard colchicine treatment, which yields at maximum 30% fertile haploids. Altogether, the results show the great potential of spontaneous chromosome doubling to streamline development DH lines and to enable new breeding schemes with more efficient allocation of resources.</description><subject>Agriculture</subject><subject>Antimitotic agents</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biometrics</subject><subject>Biotechnology</subject><subject>Breeding</subject><subject>Chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>Colchicine</subject><subject>Corn</subject><subject>Crosses, Genetic</subject><subject>Epistasis</subject><subject>Fertility</subject><subject>Fertility - genetics</subject><subject>Genetic crosses</subject><subject>Genotype</subject><subject>Haploidy</subject><subject>Heredity</subject><subject>Heritability</subject><subject>Life Sciences</subject><subject>Original Article</subject><subject>Plant Biochemistry</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Pollen - genetics</subject><subject>Quantitative genetics</subject><subject>Quantitative trait loci</subject><subject>Selection, Genetic</subject><subject>Zea mays - genetics</subject><subject>Zea mays - physiology</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kltrFTEUhYMo9lj9Az5IwBd9mJrLZMY8lqK2UBC8PIecZOeYkkmOSaYXwf9uTqdajojkIbDzrU3WYiH0nJIjSsj4phBCGesIlR3hXPDu6gFa0Z6zjrGePUQrQnrSiVGwA_SklAtCCBOEP0YHnJKBkV6s0M9TvQ3JWzzpANhBrj74eoN1tLhsU6w6QpoLNt9ymlJJE2Cb5nXwcYPhUodZV7DYR6yx9ToECLfSDGbOGWLFBQKY6lPEcL2F7KfdsPGT9j_gKXrkdCjw7O4-RF_fv_tyctqdf_xwdnJ83pl-HGon1oICEU6Pshlg3FnqpICBjdRSKwbuqO2pWTs5SG3GQbw1WmvbEwPOagn8EL1a9m5z-j5DqWryxUAIizvFGGeUUz6whr78C71Ic47td41iRLAW6nBPbVpsykeXatZmt1QdC8mZpFLKRh39g2rHwuRNiuB8m-8JXu8JGlPhum70XIo6-_xpn2ULa3IqJYNT25auzjeKErXrh1r6oVo_1G0_1FUTvbhzN68nsH8kvwvRAL4ApT3FDeR7-_9Z-wvhGsYa</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Molenaar, Willem S.</creator><creator>Schipprack, Wolfgang</creator><creator>Brauner, Pedro C.</creator><creator>Melchinger, Albrecht E.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0810-873X</orcidid></search><sort><creationdate>20190801</creationdate><title>Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize</title><author>Molenaar, Willem S. ; Schipprack, Wolfgang ; Brauner, Pedro C. ; Melchinger, Albrecht E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-5b51e05fa7925023fd1f95e6271d1d563f1d41cbf969ac7658caaad40cefda9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Agriculture</topic><topic>Antimitotic agents</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biometrics</topic><topic>Biotechnology</topic><topic>Breeding</topic><topic>Chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>Colchicine</topic><topic>Corn</topic><topic>Crosses, Genetic</topic><topic>Epistasis</topic><topic>Fertility</topic><topic>Fertility - genetics</topic><topic>Genetic crosses</topic><topic>Genotype</topic><topic>Haploidy</topic><topic>Heredity</topic><topic>Heritability</topic><topic>Life Sciences</topic><topic>Original Article</topic><topic>Plant Biochemistry</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Pollen - genetics</topic><topic>Quantitative genetics</topic><topic>Quantitative trait loci</topic><topic>Selection, Genetic</topic><topic>Zea mays - genetics</topic><topic>Zea mays - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Molenaar, Willem S.</creatorcontrib><creatorcontrib>Schipprack, Wolfgang</creatorcontrib><creatorcontrib>Brauner, Pedro C.</creatorcontrib><creatorcontrib>Melchinger, Albrecht E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Molenaar, Willem S.</au><au>Schipprack, Wolfgang</au><au>Brauner, Pedro C.</au><au>Melchinger, Albrecht E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize</atitle><jtitle>Theoretical and applied genetics</jtitle><stitle>Theor Appl Genet</stitle><addtitle>Theor Appl Genet</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>132</volume><issue>8</issue><spage>2273</spage><epage>2284</epage><pages>2273-2284</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><abstract>Key message
Mainly additive gene action governed inheritance of haploid male fertility, although epistatic effects were also significant. Recurrent selection for haploid male fertility resulted in substantial improvement in this trait.
The doubled haploid (DH) technology offers several advantages in maize breeding compared to the traditional method of recurrent selfing. However, there is still great potential for improving the success rate of DH production. Currently, the majority of haploid plants are infertile after chromosome doubling treatment by antimitotic agents such as colchicine and cannot be selfed for production of DH lines. Improvement in haploid male fertility (HMF) by selection for a higher spontaneous chromosome doubling rate (SDR) has the potential to increase DH production efficiency. To investigate the gene action governing SDR in two breeding populations, we adapted the quantitative-genetic model of Eberhart and Gardner (in Biometrics 22:864–881.
https://doi.org/10.2307/2528079
,
1966)
for the case of haploid progeny from ten DH lines and corresponding diallel crosses. Furthermore, we carried out three cycles of recurrent selection for SDR in two additional populations to evaluate the selection gain for this trait. Additive genetic effects predominated in both diallel crosses, but epistatic effects were also significant. Entry-mean heritability of SDR observed for haploid progeny of these populations exceeded 0.91, but the single-plant heritability relevant to selection was low, ranging from 0.11 to 0.19. Recurrent selection increased SDR from approximately 5–50%, which suggests the presence of few QTL with large effects. This improvement in HMF is greater than the effect of standard colchicine treatment, which yields at maximum 30% fertile haploids. Altogether, the results show the great potential of spontaneous chromosome doubling to streamline development DH lines and to enable new breeding schemes with more efficient allocation of resources.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31062045</pmid><doi>10.1007/s00122-019-03353-w</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0810-873X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0040-5752 |
| ispartof | Theoretical and applied genetics, 2019-08, Vol.132 (8), p.2273-2284 |
| issn | 0040-5752 1432-2242 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2232131362 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Antimitotic agents Biochemistry Biomedical and Life Sciences Biometrics Biotechnology Breeding Chromosomes Chromosomes, Plant - genetics Colchicine Corn Crosses, Genetic Epistasis Fertility Fertility - genetics Genetic crosses Genotype Haploidy Heredity Heritability Life Sciences Original Article Plant Biochemistry Plant Breeding/Biotechnology Plant Genetics and Genomics Pollen - genetics Quantitative genetics Quantitative trait loci Selection, Genetic Zea mays - genetics Zea mays - physiology |
| title | Haploid male fertility and spontaneous chromosome doubling evaluated in a diallel and recurrent selection experiment in maize |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T21%3A29%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Haploid%20male%20fertility%20and%20spontaneous%20chromosome%20doubling%20evaluated%20in%20a%20diallel%20and%20recurrent%20selection%20experiment%20in%20maize&rft.jtitle=Theoretical%20and%20applied%20genetics&rft.au=Molenaar,%20Willem%20S.&rft.date=2019-08-01&rft.volume=132&rft.issue=8&rft.spage=2273&rft.epage=2284&rft.pages=2273-2284&rft.issn=0040-5752&rft.eissn=1432-2242&rft_id=info:doi/10.1007/s00122-019-03353-w&rft_dat=%3Cgale_proqu%3EA593291999%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2220520046&rft_id=info:pmid/31062045&rft_galeid=A593291999&rfr_iscdi=true |