Staphylococcus debuckii sp. nov., a coagulase-negative species from bovine milk
A novel type strain, designated SDB 2975 (=CECT 9737 =DSM 105892 ), of the novel species Staphylococcus debuckii sp. nov. isolated from bovine milk is described. The novel species belongs to the genus Staphylococcus and showed resistance to tetracycline and was oxidase- and coagulase-negative, catal...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2019-08, Vol.69 (8), p.2239-2249 |
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| creator | Naushad, Sohail Kanevets, Uliana Nobrega, Diego Carson, Domonique Dufour, Simon Roy, Jean-Philippe Lewis, P Jeffrey Barkema, Herman W |
| description | A novel type strain, designated SDB 2975
(=CECT 9737
=DSM 105892
), of the novel species Staphylococcus debuckii sp. nov. isolated from bovine milk is described. The novel species belongs to the genus Staphylococcus and showed resistance to tetracycline and was oxidase- and coagulase-negative, catalase-positive, and Gram-stain-positive. Phylogenetic relationships of Staphylococcus debuckii SDB 2975
to other staphylococcal species were inferred from 16S rRNA gene and whole-genome-based phylogenetic reconstruction. The 16S rRNA gene comparisons showed that the strain is closely related to Staphylococcus condimenti (99.73 %), Staphylococcus piscifermentans (99.66 %), Staphylococcus carnosus (99.59 %) and Staphylococcus simulans (98.03 %). Average nucleotide identity (ANI) values between S.taphylococcus debuckii SDB 2975
and its closely related Staphylococcus species were 83.96, 94.5, 84.03 and 78.09 %, respectively, and digital DNA-DNA hybridization (dDDH) values were 27.70, 58.02, 27.70 and 22.00 %, respectively. The genome of Staphylococcus debuckii SDB 2975
was sequenced with PacBio and Illumina technologies and is 2 691 850 bp long, has a G+C content of 36.6 mol% and contains 2678 genes and 80 RNAs, including six copies of each5S rRNA, 16S rRNA and 23S rRNA genes. Biochemical profiling and a newly developed PCR assay enabled differentiation of Staphylococcus debuckii SDB 2975
and three other SDB strains from its closest staphylococcal species. Differentiation was also achieved by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Genes unique to Staphylococcus debuckii were identified and a PCR-based assay was developed to differentiate Staphylococcus debuckii from other staphylococcal species. In conclusion, the results of phylogenetic analysis along with the ANI values |
| doi_str_mv | 10.1099/ijsem.0.003457 |
| format | Article |
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(=CECT 9737
=DSM 105892
), of the novel species Staphylococcus debuckii sp. nov. isolated from bovine milk is described. The novel species belongs to the genus Staphylococcus and showed resistance to tetracycline and was oxidase- and coagulase-negative, catalase-positive, and Gram-stain-positive. Phylogenetic relationships of Staphylococcus debuckii SDB 2975
to other staphylococcal species were inferred from 16S rRNA gene and whole-genome-based phylogenetic reconstruction. The 16S rRNA gene comparisons showed that the strain is closely related to Staphylococcus condimenti (99.73 %), Staphylococcus piscifermentans (99.66 %), Staphylococcus carnosus (99.59 %) and Staphylococcus simulans (98.03 %). Average nucleotide identity (ANI) values between S.taphylococcus debuckii SDB 2975
and its closely related Staphylococcus species were 83.96, 94.5, 84.03 and 78.09 %, respectively, and digital DNA-DNA hybridization (dDDH) values were 27.70, 58.02, 27.70 and 22.00 %, respectively. The genome of Staphylococcus debuckii SDB 2975
was sequenced with PacBio and Illumina technologies and is 2 691 850 bp long, has a G+C content of 36.6 mol% and contains 2678 genes and 80 RNAs, including six copies of each5S rRNA, 16S rRNA and 23S rRNA genes. Biochemical profiling and a newly developed PCR assay enabled differentiation of Staphylococcus debuckii SDB 2975
and three other SDB strains from its closest staphylococcal species. Differentiation was also achieved by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Genes unique to Staphylococcus debuckii were identified and a PCR-based assay was developed to differentiate Staphylococcus debuckii from other staphylococcal species. In conclusion, the results of phylogenetic analysis along with the ANI values <95 %, and dDDH values <70 % from closely related species along with the phenotypic and biochemical characteristics and specific MALDI-TOF profiles demonstrated that Staphylococcus debuckii SDB 2975
represents a novel species within the genus Staphylococcus, named Staphylococcus debuckii sp. nov. (SDB 2975
=CECT 9737
=DSM 105892
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(=CECT 9737
=DSM 105892
), of the novel species Staphylococcus debuckii sp. nov. isolated from bovine milk is described. The novel species belongs to the genus Staphylococcus and showed resistance to tetracycline and was oxidase- and coagulase-negative, catalase-positive, and Gram-stain-positive. Phylogenetic relationships of Staphylococcus debuckii SDB 2975
to other staphylococcal species were inferred from 16S rRNA gene and whole-genome-based phylogenetic reconstruction. The 16S rRNA gene comparisons showed that the strain is closely related to Staphylococcus condimenti (99.73 %), Staphylococcus piscifermentans (99.66 %), Staphylococcus carnosus (99.59 %) and Staphylococcus simulans (98.03 %). Average nucleotide identity (ANI) values between S.taphylococcus debuckii SDB 2975
and its closely related Staphylococcus species were 83.96, 94.5, 84.03 and 78.09 %, respectively, and digital DNA-DNA hybridization (dDDH) values were 27.70, 58.02, 27.70 and 22.00 %, respectively. The genome of Staphylococcus debuckii SDB 2975
was sequenced with PacBio and Illumina technologies and is 2 691 850 bp long, has a G+C content of 36.6 mol% and contains 2678 genes and 80 RNAs, including six copies of each5S rRNA, 16S rRNA and 23S rRNA genes. Biochemical profiling and a newly developed PCR assay enabled differentiation of Staphylococcus debuckii SDB 2975
and three other SDB strains from its closest staphylococcal species. Differentiation was also achieved by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Genes unique to Staphylococcus debuckii were identified and a PCR-based assay was developed to differentiate Staphylococcus debuckii from other staphylococcal species. In conclusion, the results of phylogenetic analysis along with the ANI values <95 %, and dDDH values <70 % from closely related species along with the phenotypic and biochemical characteristics and specific MALDI-TOF profiles demonstrated that Staphylococcus debuckii SDB 2975
represents a novel species within the genus Staphylococcus, named Staphylococcus debuckii sp. nov. (SDB 2975
=CECT 9737
=DSM 105892
).</description><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo9kDtPwzAURi0EoqWwMiKPDCT4ETvxiBAvqVIHYLYc-6a4TeIQN5X67wm0dLqvc7_hIHRNSUqJUvd-FaFJSUoIz0R-gqY0kzIR43R67JmcoIsYV4SMC0LO0YRTygXn2RQt3jem-9rVwQZrh4gdlINde49jl-I2bNM7bLANZjnUJkLSwtJs_BbGM1gPEVd9aHAZtr4F3Ph6fYnOKlNHuDrUGfp8fvp4fE3mi5e3x4d5YjkXmwTy3DgnqFQuK5SSrqRKCONKYZmBIqtkQUpFOJWWVczKoiBMcZeTzMgMuOQzdLvP7frwPUDc6MZHC3VtWghD1IxxRhnJZTGi6R61fYixh0p3vW9Mv9OU6F-J-k-iJnovcXy4OWQPZQPuiP9b4z9UEG1G</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Naushad, Sohail</creator><creator>Kanevets, Uliana</creator><creator>Nobrega, Diego</creator><creator>Carson, Domonique</creator><creator>Dufour, Simon</creator><creator>Roy, Jean-Philippe</creator><creator>Lewis, P Jeffrey</creator><creator>Barkema, Herman W</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190801</creationdate><title>Staphylococcus debuckii sp. nov., a coagulase-negative species from bovine milk</title><author>Naushad, Sohail ; Kanevets, Uliana ; Nobrega, Diego ; Carson, Domonique ; Dufour, Simon ; Roy, Jean-Philippe ; Lewis, P Jeffrey ; Barkema, Herman W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-e77add5169d48996db1955adb5c2ae84f680b90316c2f2c6880293d704a64e363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naushad, Sohail</creatorcontrib><creatorcontrib>Kanevets, Uliana</creatorcontrib><creatorcontrib>Nobrega, Diego</creatorcontrib><creatorcontrib>Carson, Domonique</creatorcontrib><creatorcontrib>Dufour, Simon</creatorcontrib><creatorcontrib>Roy, Jean-Philippe</creatorcontrib><creatorcontrib>Lewis, P Jeffrey</creatorcontrib><creatorcontrib>Barkema, Herman W</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naushad, Sohail</au><au>Kanevets, Uliana</au><au>Nobrega, Diego</au><au>Carson, Domonique</au><au>Dufour, Simon</au><au>Roy, Jean-Philippe</au><au>Lewis, P Jeffrey</au><au>Barkema, Herman W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcus debuckii sp. nov., a coagulase-negative species from bovine milk</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>69</volume><issue>8</issue><spage>2239</spage><epage>2249</epage><pages>2239-2249</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel type strain, designated SDB 2975
(=CECT 9737
=DSM 105892
), of the novel species Staphylococcus debuckii sp. nov. isolated from bovine milk is described. The novel species belongs to the genus Staphylococcus and showed resistance to tetracycline and was oxidase- and coagulase-negative, catalase-positive, and Gram-stain-positive. Phylogenetic relationships of Staphylococcus debuckii SDB 2975
to other staphylococcal species were inferred from 16S rRNA gene and whole-genome-based phylogenetic reconstruction. The 16S rRNA gene comparisons showed that the strain is closely related to Staphylococcus condimenti (99.73 %), Staphylococcus piscifermentans (99.66 %), Staphylococcus carnosus (99.59 %) and Staphylococcus simulans (98.03 %). Average nucleotide identity (ANI) values between S.taphylococcus debuckii SDB 2975
and its closely related Staphylococcus species were 83.96, 94.5, 84.03 and 78.09 %, respectively, and digital DNA-DNA hybridization (dDDH) values were 27.70, 58.02, 27.70 and 22.00 %, respectively. The genome of Staphylococcus debuckii SDB 2975
was sequenced with PacBio and Illumina technologies and is 2 691 850 bp long, has a G+C content of 36.6 mol% and contains 2678 genes and 80 RNAs, including six copies of each5S rRNA, 16S rRNA and 23S rRNA genes. Biochemical profiling and a newly developed PCR assay enabled differentiation of Staphylococcus debuckii SDB 2975
and three other SDB strains from its closest staphylococcal species. Differentiation was also achieved by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF). Genes unique to Staphylococcus debuckii were identified and a PCR-based assay was developed to differentiate Staphylococcus debuckii from other staphylococcal species. In conclusion, the results of phylogenetic analysis along with the ANI values <95 %, and dDDH values <70 % from closely related species along with the phenotypic and biochemical characteristics and specific MALDI-TOF profiles demonstrated that Staphylococcus debuckii SDB 2975
represents a novel species within the genus Staphylococcus, named Staphylococcus debuckii sp. nov. (SDB 2975
=CECT 9737
=DSM 105892
).</abstract><cop>England</cop><pmid>31135334</pmid><doi>10.1099/ijsem.0.003457</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| title | Staphylococcus debuckii sp. nov., a coagulase-negative species from bovine milk |
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