Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls

Protein tyrosine kinases (PTKs) are key signaling molecules and important drug targets. Although the efficient recombinant production of active PTKs is important for both pharmaceutical industry and academic research, most PTKs are still obtained from conventional, expensive and time-consuming insec...

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Veröffentlicht in:	Methods in enzymology 2019, Vol.621, p.131-152
Hauptverfasser:	Díaz Galicia, M Escarlet, Aldehaiman, Abdullah, Hong, SeungBeom, Arold, Stefan T, Grünberg, Raik
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cloning, Molecular - methods Enzyme Assays - methods Escherichia coli - genetics Gene Expression Humans Models, Molecular Protein Domains Protein-Tyrosine Kinases - chemistry Protein-Tyrosine Kinases - genetics Protein-Tyrosine Kinases - metabolism Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism
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