Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining
Olfactomedin-like (OLFML) proteins are members of the olfactomedin domain–containing secreted glycoprotein (OLF) family. OLFML2A and OLFML2B are representative molecules of these glycoproteins. Olfactomedins are critical for the development and functional organization of the nervous system and retin...
Gespeichert in:
| Veröffentlicht in: | Fish physiology and biochemistry 2019-10, Vol.45 (5), p.1575-1587 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1587 |
|---|---|
| container_issue | 5 |
| container_start_page | 1575 |
| container_title | Fish physiology and biochemistry |
| container_volume | 45 |
| creator | Garza-Rodríguez, María Lourdes González-Álvarez, Rafael Mendoza Alfaro, Roberto Eduardo Pérez-Ibave, Diana Cristina Perez-Maya, Antonio Ali Luna-Muñoz, Maricela Mohamed-Noriega, Karim Arámburo-De-La-Hoz, Carlos Aguilera González, Carlos Javier Rodriguez Sanchez, Iram Pablo |
| description | Olfactomedin-like (OLFML) proteins are members of the olfactomedin domain–containing secreted glycoprotein (OLF) family. OLFML2A and OLFML2B are representative molecules of these glycoproteins. Olfactomedins are critical for the development and functional organization of the nervous system and retina, which is a highly conserved structure in vertebrates, having almost identical anatomical and physiological characteristics in multiple taxa. Spotted gar, a member of the
Lepisosteidae
family, is a freshwater fish that inhabits rivers, bayous, swamps, and brackish waters. Recently, the complete genome has been sequenced, providing a unique bridge between fish medical models to human biology, making it an excellent animal model. This study was aimed to understanding the evolution OLFML2A and OLFML2B in the retina of spotted gar through looking for the expression of these genes. Spotted gar retina was analyzed with hematoxylin-eosin staining assays to provide an overall view of the retina structure and an immunofluorescence assay to identify OLFML2A and OLFML2B protein expression. A phylogenetic tree was created using the neighbor-joining method. Forces that direct the evolution of the fish genes were tested. Spotted gar retina, as in other vertebrates, is made of several layers. OLFML2A and OLFML2B proteins were detected in the rod and cone photoreceptor layer (PRL), outer nuclear layer (ONL), and inner nuclear layer (INL). Phylogenetic tree analysis confirms the orthology within the OLFML2A gene. Purifying selection is the evolutionary force that directs the OLFML2A genes. OLFML2A genes have a well-conserved function over time and species. |
| doi_str_mv | 10.1007/s10695-019-00647-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2232084781</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2232084781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-ff06260933c1be72076d024a6504346acb53127f0f20c9c643684b9840c2cc8f3</originalsourceid><addsrcrecordid>eNp9kc-O1SAUh4nRONfRF3BhSNzcWVQPfwrtcmbiqEnN3eiaUC5cGVuoQBN9DZ9YnI6auJANEL7zO8CH0HMCrwiAfJ0JiL5tgPQNgOCygQdoR1rJmpaI7iHaQU-hIZLTM_Qk51sA6KUgj9EZI3UwInfox2Fy2pQ426MPzeS_WEzxJdbhiK_w_jDcfBjott3WVxd4SdH5yWL7bUk2Zx8D9gGXzxYnW3zQODqcl1iKPeKTTng_2MXnmItdM45mnXRZ88Vd5uijDy6mWRdvMp598OH0FD1yesr22f18jj7dvPl4_a4ZDm_fX18OjWFUlMY5EFRAz5gho5UUpDgC5Vq0wBkX2owtI1Q6cBRMbwRnouNj33Ew1JjOsXO033Lrg76uNhc1-2zsNOlg45oVpYxCx2VHKvryH_Q2rinU21WKSsFk_dlK0Y0yKeacrFNL8rNO3xUB9cuY2oypakzdGVNQi17cR69jlfCn5LeiCrANyPUonGz62_s_sT8BXxWepA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2227637097</pqid></control><display><type>article</type><title>Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Garza-Rodríguez, María Lourdes ; González-Álvarez, Rafael ; Mendoza Alfaro, Roberto Eduardo ; Pérez-Ibave, Diana Cristina ; Perez-Maya, Antonio Ali ; Luna-Muñoz, Maricela ; Mohamed-Noriega, Karim ; Arámburo-De-La-Hoz, Carlos ; Aguilera González, Carlos Javier ; Rodriguez Sanchez, Iram Pablo</creator><creatorcontrib>Garza-Rodríguez, María Lourdes ; González-Álvarez, Rafael ; Mendoza Alfaro, Roberto Eduardo ; Pérez-Ibave, Diana Cristina ; Perez-Maya, Antonio Ali ; Luna-Muñoz, Maricela ; Mohamed-Noriega, Karim ; Arámburo-De-La-Hoz, Carlos ; Aguilera González, Carlos Javier ; Rodriguez Sanchez, Iram Pablo</creatorcontrib><description>Olfactomedin-like (OLFML) proteins are members of the olfactomedin domain–containing secreted glycoprotein (OLF) family. OLFML2A and OLFML2B are representative molecules of these glycoproteins. Olfactomedins are critical for the development and functional organization of the nervous system and retina, which is a highly conserved structure in vertebrates, having almost identical anatomical and physiological characteristics in multiple taxa. Spotted gar, a member of the
Lepisosteidae
family, is a freshwater fish that inhabits rivers, bayous, swamps, and brackish waters. Recently, the complete genome has been sequenced, providing a unique bridge between fish medical models to human biology, making it an excellent animal model. This study was aimed to understanding the evolution OLFML2A and OLFML2B in the retina of spotted gar through looking for the expression of these genes. Spotted gar retina was analyzed with hematoxylin-eosin staining assays to provide an overall view of the retina structure and an immunofluorescence assay to identify OLFML2A and OLFML2B protein expression. A phylogenetic tree was created using the neighbor-joining method. Forces that direct the evolution of the fish genes were tested. Spotted gar retina, as in other vertebrates, is made of several layers. OLFML2A and OLFML2B proteins were detected in the rod and cone photoreceptor layer (PRL), outer nuclear layer (ONL), and inner nuclear layer (INL). Phylogenetic tree analysis confirms the orthology within the OLFML2A gene. Purifying selection is the evolutionary force that directs the OLFML2A genes. OLFML2A genes have a well-conserved function over time and species.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-019-00647-0</identifier><identifier>PMID: 31111317</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animal models ; Animal Physiology ; Animals ; Bayous ; Bioinformatics ; Biological evolution ; Biology ; Biomedical and Life Sciences ; Computational Biology - methods ; Data Mining - methods ; Evolution ; Evolutionary genetics ; Extracellular Matrix Proteins - genetics ; Extracellular Matrix Proteins - metabolism ; Fish ; Fishes - metabolism ; Freshwater ; Freshwater & Marine Ecology ; Freshwater fish ; Freshwater fishes ; Functional morphology ; Gene expression ; Gene Expression Regulation ; Genes ; Genomes ; Glycoproteins ; Glycoproteins - genetics ; Glycoproteins - metabolism ; Histology ; Immunofluorescence ; Inland water environment ; Lepisosteus oculatus ; Life Sciences ; Membrane Proteins ; Morphology ; Nervous system ; Olfactomedin ; Organizations ; Orthology ; Photoreceptors ; Phylogenetics ; Phylogeny ; Proteins ; Retina ; Retina - metabolism ; Rivers ; Swamps ; Transcriptome ; Vertebrates ; Zoology</subject><ispartof>Fish physiology and biochemistry, 2019-10, Vol.45 (5), p.1575-1587</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Fish Physiology and Biochemistry is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-ff06260933c1be72076d024a6504346acb53127f0f20c9c643684b9840c2cc8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10695-019-00647-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10695-019-00647-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31111317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garza-Rodríguez, María Lourdes</creatorcontrib><creatorcontrib>González-Álvarez, Rafael</creatorcontrib><creatorcontrib>Mendoza Alfaro, Roberto Eduardo</creatorcontrib><creatorcontrib>Pérez-Ibave, Diana Cristina</creatorcontrib><creatorcontrib>Perez-Maya, Antonio Ali</creatorcontrib><creatorcontrib>Luna-Muñoz, Maricela</creatorcontrib><creatorcontrib>Mohamed-Noriega, Karim</creatorcontrib><creatorcontrib>Arámburo-De-La-Hoz, Carlos</creatorcontrib><creatorcontrib>Aguilera González, Carlos Javier</creatorcontrib><creatorcontrib>Rodriguez Sanchez, Iram Pablo</creatorcontrib><title>Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining</title><title>Fish physiology and biochemistry</title><addtitle>Fish Physiol Biochem</addtitle><addtitle>Fish Physiol Biochem</addtitle><description>Olfactomedin-like (OLFML) proteins are members of the olfactomedin domain–containing secreted glycoprotein (OLF) family. OLFML2A and OLFML2B are representative molecules of these glycoproteins. Olfactomedins are critical for the development and functional organization of the nervous system and retina, which is a highly conserved structure in vertebrates, having almost identical anatomical and physiological characteristics in multiple taxa. Spotted gar, a member of the
Lepisosteidae
family, is a freshwater fish that inhabits rivers, bayous, swamps, and brackish waters. Recently, the complete genome has been sequenced, providing a unique bridge between fish medical models to human biology, making it an excellent animal model. This study was aimed to understanding the evolution OLFML2A and OLFML2B in the retina of spotted gar through looking for the expression of these genes. Spotted gar retina was analyzed with hematoxylin-eosin staining assays to provide an overall view of the retina structure and an immunofluorescence assay to identify OLFML2A and OLFML2B protein expression. A phylogenetic tree was created using the neighbor-joining method. Forces that direct the evolution of the fish genes were tested. Spotted gar retina, as in other vertebrates, is made of several layers. OLFML2A and OLFML2B proteins were detected in the rod and cone photoreceptor layer (PRL), outer nuclear layer (ONL), and inner nuclear layer (INL). Phylogenetic tree analysis confirms the orthology within the OLFML2A gene. Purifying selection is the evolutionary force that directs the OLFML2A genes. OLFML2A genes have a well-conserved function over time and species.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal models</subject><subject>Animal Physiology</subject><subject>Animals</subject><subject>Bayous</subject><subject>Bioinformatics</subject><subject>Biological evolution</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Computational Biology - methods</subject><subject>Data Mining - methods</subject><subject>Evolution</subject><subject>Evolutionary genetics</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Fish</subject><subject>Fishes - metabolism</subject><subject>Freshwater</subject><subject>Freshwater & Marine Ecology</subject><subject>Freshwater fish</subject><subject>Freshwater fishes</subject><subject>Functional morphology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genomes</subject><subject>Glycoproteins</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>Histology</subject><subject>Immunofluorescence</subject><subject>Inland water environment</subject><subject>Lepisosteus oculatus</subject><subject>Life Sciences</subject><subject>Membrane Proteins</subject><subject>Morphology</subject><subject>Nervous system</subject><subject>Olfactomedin</subject><subject>Organizations</subject><subject>Orthology</subject><subject>Photoreceptors</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Retina</subject><subject>Retina - metabolism</subject><subject>Rivers</subject><subject>Swamps</subject><subject>Transcriptome</subject><subject>Vertebrates</subject><subject>Zoology</subject><issn>0920-1742</issn><issn>1573-5168</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc-O1SAUh4nRONfRF3BhSNzcWVQPfwrtcmbiqEnN3eiaUC5cGVuoQBN9DZ9YnI6auJANEL7zO8CH0HMCrwiAfJ0JiL5tgPQNgOCygQdoR1rJmpaI7iHaQU-hIZLTM_Qk51sA6KUgj9EZI3UwInfox2Fy2pQ426MPzeS_WEzxJdbhiK_w_jDcfBjott3WVxd4SdH5yWL7bUk2Zx8D9gGXzxYnW3zQODqcl1iKPeKTTng_2MXnmItdM45mnXRZ88Vd5uijDy6mWRdvMp598OH0FD1yesr22f18jj7dvPl4_a4ZDm_fX18OjWFUlMY5EFRAz5gho5UUpDgC5Vq0wBkX2owtI1Q6cBRMbwRnouNj33Ew1JjOsXO033Lrg76uNhc1-2zsNOlg45oVpYxCx2VHKvryH_Q2rinU21WKSsFk_dlK0Y0yKeacrFNL8rNO3xUB9cuY2oypakzdGVNQi17cR69jlfCn5LeiCrANyPUonGz62_s_sT8BXxWepA</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Garza-Rodríguez, María Lourdes</creator><creator>González-Álvarez, Rafael</creator><creator>Mendoza Alfaro, Roberto Eduardo</creator><creator>Pérez-Ibave, Diana Cristina</creator><creator>Perez-Maya, Antonio Ali</creator><creator>Luna-Muñoz, Maricela</creator><creator>Mohamed-Noriega, Karim</creator><creator>Arámburo-De-La-Hoz, Carlos</creator><creator>Aguilera González, Carlos Javier</creator><creator>Rodriguez Sanchez, Iram Pablo</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20191001</creationdate><title>Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining</title><author>Garza-Rodríguez, María Lourdes ; González-Álvarez, Rafael ; Mendoza Alfaro, Roberto Eduardo ; Pérez-Ibave, Diana Cristina ; Perez-Maya, Antonio Ali ; Luna-Muñoz, Maricela ; Mohamed-Noriega, Karim ; Arámburo-De-La-Hoz, Carlos ; Aguilera González, Carlos Javier ; Rodriguez Sanchez, Iram Pablo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-ff06260933c1be72076d024a6504346acb53127f0f20c9c643684b9840c2cc8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animal models</topic><topic>Animal Physiology</topic><topic>Animals</topic><topic>Bayous</topic><topic>Bioinformatics</topic><topic>Biological evolution</topic><topic>Biology</topic><topic>Biomedical and Life Sciences</topic><topic>Computational Biology - methods</topic><topic>Data Mining - methods</topic><topic>Evolution</topic><topic>Evolutionary genetics</topic><topic>Extracellular Matrix Proteins - genetics</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Fish</topic><topic>Fishes - metabolism</topic><topic>Freshwater</topic><topic>Freshwater & Marine Ecology</topic><topic>Freshwater fish</topic><topic>Freshwater fishes</topic><topic>Functional morphology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Genomes</topic><topic>Glycoproteins</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>Histology</topic><topic>Immunofluorescence</topic><topic>Inland water environment</topic><topic>Lepisosteus oculatus</topic><topic>Life Sciences</topic><topic>Membrane Proteins</topic><topic>Morphology</topic><topic>Nervous system</topic><topic>Olfactomedin</topic><topic>Organizations</topic><topic>Orthology</topic><topic>Photoreceptors</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Proteins</topic><topic>Retina</topic><topic>Retina - metabolism</topic><topic>Rivers</topic><topic>Swamps</topic><topic>Transcriptome</topic><topic>Vertebrates</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garza-Rodríguez, María Lourdes</creatorcontrib><creatorcontrib>González-Álvarez, Rafael</creatorcontrib><creatorcontrib>Mendoza Alfaro, Roberto Eduardo</creatorcontrib><creatorcontrib>Pérez-Ibave, Diana Cristina</creatorcontrib><creatorcontrib>Perez-Maya, Antonio Ali</creatorcontrib><creatorcontrib>Luna-Muñoz, Maricela</creatorcontrib><creatorcontrib>Mohamed-Noriega, Karim</creatorcontrib><creatorcontrib>Arámburo-De-La-Hoz, Carlos</creatorcontrib><creatorcontrib>Aguilera González, Carlos Javier</creatorcontrib><creatorcontrib>Rodriguez Sanchez, Iram Pablo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fish physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garza-Rodríguez, María Lourdes</au><au>González-Álvarez, Rafael</au><au>Mendoza Alfaro, Roberto Eduardo</au><au>Pérez-Ibave, Diana Cristina</au><au>Perez-Maya, Antonio Ali</au><au>Luna-Muñoz, Maricela</au><au>Mohamed-Noriega, Karim</au><au>Arámburo-De-La-Hoz, Carlos</au><au>Aguilera González, Carlos Javier</au><au>Rodriguez Sanchez, Iram Pablo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><addtitle>Fish Physiol Biochem</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>45</volume><issue>5</issue><spage>1575</spage><epage>1587</epage><pages>1575-1587</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>Olfactomedin-like (OLFML) proteins are members of the olfactomedin domain–containing secreted glycoprotein (OLF) family. OLFML2A and OLFML2B are representative molecules of these glycoproteins. Olfactomedins are critical for the development and functional organization of the nervous system and retina, which is a highly conserved structure in vertebrates, having almost identical anatomical and physiological characteristics in multiple taxa. Spotted gar, a member of the
Lepisosteidae
family, is a freshwater fish that inhabits rivers, bayous, swamps, and brackish waters. Recently, the complete genome has been sequenced, providing a unique bridge between fish medical models to human biology, making it an excellent animal model. This study was aimed to understanding the evolution OLFML2A and OLFML2B in the retina of spotted gar through looking for the expression of these genes. Spotted gar retina was analyzed with hematoxylin-eosin staining assays to provide an overall view of the retina structure and an immunofluorescence assay to identify OLFML2A and OLFML2B protein expression. A phylogenetic tree was created using the neighbor-joining method. Forces that direct the evolution of the fish genes were tested. Spotted gar retina, as in other vertebrates, is made of several layers. OLFML2A and OLFML2B proteins were detected in the rod and cone photoreceptor layer (PRL), outer nuclear layer (ONL), and inner nuclear layer (INL). Phylogenetic tree analysis confirms the orthology within the OLFML2A gene. Purifying selection is the evolutionary force that directs the OLFML2A genes. OLFML2A genes have a well-conserved function over time and species.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31111317</pmid><doi>10.1007/s10695-019-00647-0</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-1742 |
| ispartof | Fish physiology and biochemistry, 2019-10, Vol.45 (5), p.1575-1587 |
| issn | 0920-1742 1573-5168 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2232084781 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animal Anatomy Animal Biochemistry Animal models Animal Physiology Animals Bayous Bioinformatics Biological evolution Biology Biomedical and Life Sciences Computational Biology - methods Data Mining - methods Evolution Evolutionary genetics Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - metabolism Fish Fishes - metabolism Freshwater Freshwater & Marine Ecology Freshwater fish Freshwater fishes Functional morphology Gene expression Gene Expression Regulation Genes Genomes Glycoproteins Glycoproteins - genetics Glycoproteins - metabolism Histology Immunofluorescence Inland water environment Lepisosteus oculatus Life Sciences Membrane Proteins Morphology Nervous system Olfactomedin Organizations Orthology Photoreceptors Phylogenetics Phylogeny Proteins Retina Retina - metabolism Rivers Swamps Transcriptome Vertebrates Zoology |
| title | Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T22%3A22%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Olfactomedin-like%202%20A%20and%20B%20(OLFML2A%20and%20OLFML2B)%20profile%20expression%20in%20the%20retina%20of%20spotted%20gar%20(Lepisosteus%20oculatus)%20and%20bioinformatics%20mining&rft.jtitle=Fish%20physiology%20and%20biochemistry&rft.au=Garza-Rodr%C3%ADguez,%20Mar%C3%ADa%20Lourdes&rft.date=2019-10-01&rft.volume=45&rft.issue=5&rft.spage=1575&rft.epage=1587&rft.pages=1575-1587&rft.issn=0920-1742&rft.eissn=1573-5168&rft_id=info:doi/10.1007/s10695-019-00647-0&rft_dat=%3Cproquest_cross%3E2232084781%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2227637097&rft_id=info:pmid/31111317&rfr_iscdi=true |