Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms
A new method on RP-HPLC is devised and validated, as per ICH guidelines, for the synchronous estimation of cefpodoxime proxetil and H2-receptor antagonits that are Cimetidine, Famotidine and Ranitidine. The method is simple, accurate, expeditious, reproducible, robust and precise. Chromatography was...
Gespeichert in:
| Veröffentlicht in: | Pakistan journal of pharmaceutical sciences 2019-03, Vol.32 (2 (Supplementary)), p.839-844 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 844 |
|---|---|
| container_issue | 2 (Supplementary) |
| container_start_page | 839 |
| container_title | Pakistan journal of pharmaceutical sciences |
| container_volume | 32 |
| creator | Hassan, Sohail Iqbal, Sadia Zaheer, Erum Hassan, Amir Hamid, Shaista Ali, Mohsin Akram, Arfa Maroof, Syed Zohaib Abedin, Saima Khan, Sidra J |
| description | A new method on RP-HPLC is devised and validated, as per ICH guidelines, for the synchronous estimation of cefpodoxime proxetil and H2-receptor antagonits that are Cimetidine, Famotidine and Ranitidine. The method is simple, accurate, expeditious, reproducible, robust and precise. Chromatography was done on a C
(250 x 4.6mm) column with methanol: water as mobile phae in the ratio of 70:30 (v/v), pumped at a flow rate of 1ml/min and pH was maintained using 85% ortho-phosphoric acid at 3. The λ max 240 nm was preferred for UV detection. A good linear relationship was attained, over the concentration ranges of 20-70 μg/ml and 5-30μg/ml, for cefpodoxime proxetil and H
blockers respectively, with a correlation coefficient of R= 0.9987 to 0.9992. The method was validated and found precised (i.e. intra day and interday analysis) with RSD |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_2232068381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2232068381</sourcerecordid><originalsourceid>FETCH-LOGICAL-p126t-30d7b62f0146fa48569f3588ba536285cf87a23f4548faba5d43e1d6f749d74c3</originalsourceid><addsrcrecordid>eNo9kM1OwzAQhHMA0VJ4BeQjl0j-i-McUfkpUiUqBBK3yI3XrZEdh9ipysvwrARaOK20mvl2Zk-yKcGE5AKTt0l2HuM7xoJXVXWWTRghmFUST7OvW9iBC52HNiHVarRTzmqVbGhRMOh5lS9WyznykLZBIxN6FK0fXFIthCEiDQl6b9t_QwOmCzrsrQfU9WEPybpf7oKiHhro0ohQbVKb0NqYIrIt6raq96qBIdlGOaRDVBv4ueXjRXZqlItweZyz7PX-7mW-yJdPD4_zm2XeESpSzrAu14IaTLgwistCVIYVUq5VwQSVRWNkqSgzvODSqHGrOQOihSl5pUvesFl2feCOmT8GiKn2Njbg3KFnTSmjWEgmySi9OkqHtQddd731qv-s_37KvgECLnVE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2232068381</pqid></control><display><type>article</type><title>Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Hassan, Sohail ; Iqbal, Sadia ; Zaheer, Erum ; Hassan, Amir ; Hamid, Shaista ; Ali, Mohsin ; Akram, Arfa ; Maroof, Syed Zohaib ; Abedin, Saima ; Khan, Sidra J</creator><creatorcontrib>Hassan, Sohail ; Iqbal, Sadia ; Zaheer, Erum ; Hassan, Amir ; Hamid, Shaista ; Ali, Mohsin ; Akram, Arfa ; Maroof, Syed Zohaib ; Abedin, Saima ; Khan, Sidra J</creatorcontrib><description>A new method on RP-HPLC is devised and validated, as per ICH guidelines, for the synchronous estimation of cefpodoxime proxetil and H2-receptor antagonits that are Cimetidine, Famotidine and Ranitidine. The method is simple, accurate, expeditious, reproducible, robust and precise. Chromatography was done on a C
(250 x 4.6mm) column with methanol: water as mobile phae in the ratio of 70:30 (v/v), pumped at a flow rate of 1ml/min and pH was maintained using 85% ortho-phosphoric acid at 3. The λ max 240 nm was preferred for UV detection. A good linear relationship was attained, over the concentration ranges of 20-70 μg/ml and 5-30μg/ml, for cefpodoxime proxetil and H
blockers respectively, with a correlation coefficient of R= 0.9987 to 0.9992. The method was validated and found precised (i.e. intra day and interday analysis) with RSD <2%. LOD and LOQ observations were under 0.4806 to 2.6069μg/ml which proved the method to be sensitive. The method provided satisfactory results of robustness and reproducibility, when validated and applied successfully for analysis of dosage forms.</description><identifier>ISSN: 1011-601X</identifier><identifier>PMID: 31103980</identifier><language>eng</language><publisher>Pakistan</publisher><subject>Cefpodoxime Proxetil ; Ceftizoxime - analogs & derivatives ; Ceftizoxime - analysis ; Chromatography, High Pressure Liquid - methods ; Chromatography, Reverse-Phase - methods ; Cimetidine - analysis ; Dosage Forms ; Famotidine - analysis ; Histamine H2 Antagonists - analysis ; Limit of Detection ; Ranitidine - analysis ; Tablets - analysis</subject><ispartof>Pakistan journal of pharmaceutical sciences, 2019-03, Vol.32 (2 (Supplementary)), p.839-844</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31103980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hassan, Sohail</creatorcontrib><creatorcontrib>Iqbal, Sadia</creatorcontrib><creatorcontrib>Zaheer, Erum</creatorcontrib><creatorcontrib>Hassan, Amir</creatorcontrib><creatorcontrib>Hamid, Shaista</creatorcontrib><creatorcontrib>Ali, Mohsin</creatorcontrib><creatorcontrib>Akram, Arfa</creatorcontrib><creatorcontrib>Maroof, Syed Zohaib</creatorcontrib><creatorcontrib>Abedin, Saima</creatorcontrib><creatorcontrib>Khan, Sidra J</creatorcontrib><title>Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms</title><title>Pakistan journal of pharmaceutical sciences</title><addtitle>Pak J Pharm Sci</addtitle><description>A new method on RP-HPLC is devised and validated, as per ICH guidelines, for the synchronous estimation of cefpodoxime proxetil and H2-receptor antagonits that are Cimetidine, Famotidine and Ranitidine. The method is simple, accurate, expeditious, reproducible, robust and precise. Chromatography was done on a C
(250 x 4.6mm) column with methanol: water as mobile phae in the ratio of 70:30 (v/v), pumped at a flow rate of 1ml/min and pH was maintained using 85% ortho-phosphoric acid at 3. The λ max 240 nm was preferred for UV detection. A good linear relationship was attained, over the concentration ranges of 20-70 μg/ml and 5-30μg/ml, for cefpodoxime proxetil and H
blockers respectively, with a correlation coefficient of R= 0.9987 to 0.9992. The method was validated and found precised (i.e. intra day and interday analysis) with RSD <2%. LOD and LOQ observations were under 0.4806 to 2.6069μg/ml which proved the method to be sensitive. The method provided satisfactory results of robustness and reproducibility, when validated and applied successfully for analysis of dosage forms.</description><subject>Cefpodoxime Proxetil</subject><subject>Ceftizoxime - analogs & derivatives</subject><subject>Ceftizoxime - analysis</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Reverse-Phase - methods</subject><subject>Cimetidine - analysis</subject><subject>Dosage Forms</subject><subject>Famotidine - analysis</subject><subject>Histamine H2 Antagonists - analysis</subject><subject>Limit of Detection</subject><subject>Ranitidine - analysis</subject><subject>Tablets - analysis</subject><issn>1011-601X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1OwzAQhHMA0VJ4BeQjl0j-i-McUfkpUiUqBBK3yI3XrZEdh9ipysvwrARaOK20mvl2Zk-yKcGE5AKTt0l2HuM7xoJXVXWWTRghmFUST7OvW9iBC52HNiHVarRTzmqVbGhRMOh5lS9WyznykLZBIxN6FK0fXFIthCEiDQl6b9t_QwOmCzrsrQfU9WEPybpf7oKiHhro0ohQbVKb0NqYIrIt6raq96qBIdlGOaRDVBv4ueXjRXZqlItweZyz7PX-7mW-yJdPD4_zm2XeESpSzrAu14IaTLgwistCVIYVUq5VwQSVRWNkqSgzvODSqHGrOQOihSl5pUvesFl2feCOmT8GiKn2Njbg3KFnTSmjWEgmySi9OkqHtQddd731qv-s_37KvgECLnVE</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Hassan, Sohail</creator><creator>Iqbal, Sadia</creator><creator>Zaheer, Erum</creator><creator>Hassan, Amir</creator><creator>Hamid, Shaista</creator><creator>Ali, Mohsin</creator><creator>Akram, Arfa</creator><creator>Maroof, Syed Zohaib</creator><creator>Abedin, Saima</creator><creator>Khan, Sidra J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201903</creationdate><title>Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms</title><author>Hassan, Sohail ; Iqbal, Sadia ; Zaheer, Erum ; Hassan, Amir ; Hamid, Shaista ; Ali, Mohsin ; Akram, Arfa ; Maroof, Syed Zohaib ; Abedin, Saima ; Khan, Sidra J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-30d7b62f0146fa48569f3588ba536285cf87a23f4548faba5d43e1d6f749d74c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Cefpodoxime Proxetil</topic><topic>Ceftizoxime - analogs & derivatives</topic><topic>Ceftizoxime - analysis</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Reverse-Phase - methods</topic><topic>Cimetidine - analysis</topic><topic>Dosage Forms</topic><topic>Famotidine - analysis</topic><topic>Histamine H2 Antagonists - analysis</topic><topic>Limit of Detection</topic><topic>Ranitidine - analysis</topic><topic>Tablets - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassan, Sohail</creatorcontrib><creatorcontrib>Iqbal, Sadia</creatorcontrib><creatorcontrib>Zaheer, Erum</creatorcontrib><creatorcontrib>Hassan, Amir</creatorcontrib><creatorcontrib>Hamid, Shaista</creatorcontrib><creatorcontrib>Ali, Mohsin</creatorcontrib><creatorcontrib>Akram, Arfa</creatorcontrib><creatorcontrib>Maroof, Syed Zohaib</creatorcontrib><creatorcontrib>Abedin, Saima</creatorcontrib><creatorcontrib>Khan, Sidra J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassan, Sohail</au><au>Iqbal, Sadia</au><au>Zaheer, Erum</au><au>Hassan, Amir</au><au>Hamid, Shaista</au><au>Ali, Mohsin</au><au>Akram, Arfa</au><au>Maroof, Syed Zohaib</au><au>Abedin, Saima</au><au>Khan, Sidra J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms</atitle><jtitle>Pakistan journal of pharmaceutical sciences</jtitle><addtitle>Pak J Pharm Sci</addtitle><date>2019-03</date><risdate>2019</risdate><volume>32</volume><issue>2 (Supplementary)</issue><spage>839</spage><epage>844</epage><pages>839-844</pages><issn>1011-601X</issn><abstract>A new method on RP-HPLC is devised and validated, as per ICH guidelines, for the synchronous estimation of cefpodoxime proxetil and H2-receptor antagonits that are Cimetidine, Famotidine and Ranitidine. The method is simple, accurate, expeditious, reproducible, robust and precise. Chromatography was done on a C
(250 x 4.6mm) column with methanol: water as mobile phae in the ratio of 70:30 (v/v), pumped at a flow rate of 1ml/min and pH was maintained using 85% ortho-phosphoric acid at 3. The λ max 240 nm was preferred for UV detection. A good linear relationship was attained, over the concentration ranges of 20-70 μg/ml and 5-30μg/ml, for cefpodoxime proxetil and H
blockers respectively, with a correlation coefficient of R= 0.9987 to 0.9992. The method was validated and found precised (i.e. intra day and interday analysis) with RSD <2%. LOD and LOQ observations were under 0.4806 to 2.6069μg/ml which proved the method to be sensitive. The method provided satisfactory results of robustness and reproducibility, when validated and applied successfully for analysis of dosage forms.</abstract><cop>Pakistan</cop><pmid>31103980</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1011-601X |
| ispartof | Pakistan journal of pharmaceutical sciences, 2019-03, Vol.32 (2 (Supplementary)), p.839-844 |
| issn | 1011-601X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2232068381 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Cefpodoxime Proxetil Ceftizoxime - analogs & derivatives Ceftizoxime - analysis Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase - methods Cimetidine - analysis Dosage Forms Famotidine - analysis Histamine H2 Antagonists - analysis Limit of Detection Ranitidine - analysis Tablets - analysis |
| title | Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T22%3A35%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20validation%20of%20RP-HPLC%20method%20for%20simultaneous%20determination%20of%20cefpodoxime%20proxetil%20and%20H2%20receptor%20antagonists%20in%20pharmaceutical%20dosage%20forms&rft.jtitle=Pakistan%20journal%20of%20pharmaceutical%20sciences&rft.au=Hassan,%20Sohail&rft.date=2019-03&rft.volume=32&rft.issue=2%20(Supplementary)&rft.spage=839&rft.epage=844&rft.pages=839-844&rft.issn=1011-601X&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E2232068381%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2232068381&rft_id=info:pmid/31103980&rfr_iscdi=true |