Diethylstilbestrol induces morphological changes in the spermatogonia, Sertoli cells and Leydig cells of adult rat
It is now established that diethylstilbestrol (DES) has damaging effects on the male reproductive system. However, to date there have been no studies morphological analysis of adult rat testes upon treatment with DES. Here, we examined whether DES has any significant morphological effect on steroido...
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| Veröffentlicht in: | Research in veterinary science 2019-06, Vol.124, p.433-438 |
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| creator | Rahman, Md. Mahfujur Wie, Jehyung Cho, Jeong-Hwi Tae, Hyun-Jin Ahn, Dongchoon Lee, Seok Won Kim, In-Shik Park, Byung-Yong |
| description | It is now established that diethylstilbestrol (DES) has damaging effects on the male reproductive system. However, to date there have been no studies morphological analysis of adult rat testes upon treatment with DES. Here, we examined whether DES has any significant morphological effect on steroidogenesis and spermatogenesis. DES was injected subcutaneously at 3 μg/day and 30 μg/day in adult male Sprague-Dawley (SD) rats for two different treatment lengths (1 or 3 weeks), after which rats were necropsied. TUNEL labeling, cell counting, and morphological analysis were used to evaluate the effects of DES. A high dose of DES and longer exposure severely affected the cellular development of the testis. Specifically, DES treatment disrupted both steroidogenesis and spermatogenesis by decreasing the number of spermatogonia, Sertoli cells, and Leydig cells in a dose- and time-dependent manner. Thus, DES may account for decreases in the number of spermatogenic cells, Sertoli cells and Leydig cells, which in turn may lead to reduced fertility in males.
•Diethylstilbestrol treatment disrupted both steroidogenesis and spermatogenesis.•Diethylstilbestrol treatment decreased the number of spermatogonia, Sertoli cells, Leydig cells.•Diethylstilbestrol may lead to reduced fertility in males. |
| doi_str_mv | 10.1016/j.rvsc.2019.04.015 |
| format | Article |
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•Diethylstilbestrol treatment disrupted both steroidogenesis and spermatogenesis.•Diethylstilbestrol treatment decreased the number of spermatogonia, Sertoli cells, Leydig cells.•Diethylstilbestrol may lead to reduced fertility in males.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2019.04.015</identifier><identifier>PMID: 31082573</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adult rat ; Apoptosis ; Diethylstibestrol ; Diethylstilbestrol ; Estrogens ; Fertility ; Labeling ; Laboratory animals ; Leydig cells ; Males ; Microscopy ; Morphology ; Reproductive system ; Rodents ; Sertoli cells ; Spermatogenesis ; Spermatogonia ; Steroidogenesis ; Testes ; Time dependence ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2019-06, Vol.124, p.433-438</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Jun 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-266a9aa9422af2cc592ecf6f9db8d2961474ccc51215d6249a8f8a27b56bf91d3</citedby><cites>FETCH-LOGICAL-c384t-266a9aa9422af2cc592ecf6f9db8d2961474ccc51215d6249a8f8a27b56bf91d3</cites><orcidid>0000-0001-8897-2258</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0034528818314243$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31082573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahman, Md. Mahfujur</creatorcontrib><creatorcontrib>Wie, Jehyung</creatorcontrib><creatorcontrib>Cho, Jeong-Hwi</creatorcontrib><creatorcontrib>Tae, Hyun-Jin</creatorcontrib><creatorcontrib>Ahn, Dongchoon</creatorcontrib><creatorcontrib>Lee, Seok Won</creatorcontrib><creatorcontrib>Kim, In-Shik</creatorcontrib><creatorcontrib>Park, Byung-Yong</creatorcontrib><title>Diethylstilbestrol induces morphological changes in the spermatogonia, Sertoli cells and Leydig cells of adult rat</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>It is now established that diethylstilbestrol (DES) has damaging effects on the male reproductive system. However, to date there have been no studies morphological analysis of adult rat testes upon treatment with DES. Here, we examined whether DES has any significant morphological effect on steroidogenesis and spermatogenesis. DES was injected subcutaneously at 3 μg/day and 30 μg/day in adult male Sprague-Dawley (SD) rats for two different treatment lengths (1 or 3 weeks), after which rats were necropsied. TUNEL labeling, cell counting, and morphological analysis were used to evaluate the effects of DES. A high dose of DES and longer exposure severely affected the cellular development of the testis. Specifically, DES treatment disrupted both steroidogenesis and spermatogenesis by decreasing the number of spermatogonia, Sertoli cells, and Leydig cells in a dose- and time-dependent manner. Thus, DES may account for decreases in the number of spermatogenic cells, Sertoli cells and Leydig cells, which in turn may lead to reduced fertility in males.
•Diethylstilbestrol treatment disrupted both steroidogenesis and spermatogenesis.•Diethylstilbestrol treatment decreased the number of spermatogonia, Sertoli cells, Leydig cells.•Diethylstilbestrol may lead to reduced fertility in males.</description><subject>Adult rat</subject><subject>Apoptosis</subject><subject>Diethylstibestrol</subject><subject>Diethylstilbestrol</subject><subject>Estrogens</subject><subject>Fertility</subject><subject>Labeling</subject><subject>Laboratory animals</subject><subject>Leydig cells</subject><subject>Males</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Reproductive system</subject><subject>Rodents</subject><subject>Sertoli cells</subject><subject>Spermatogenesis</subject><subject>Spermatogonia</subject><subject>Steroidogenesis</subject><subject>Testes</subject><subject>Time dependence</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kc2KFDEUhYM4OO3oC7iQgBsXdpm_SlfAzTD-zECDC3UdUsmt7jSpSpukBvrtTdGtCxezChy-e7g3H0JvKGkoofLjoUmP2TaMUNUQ0RDaPkMr2nK2ZlLS52hFCBfrlnXdNXqZ84EQIijdvEDXnJKOtRu-Qumzh7I_hVx86CGXFAP2k5stZDzGdNzHEHfemoDt3ky7mvoJlz3gfIQ0mhJ3cfLmA_4BqcTgsYUQMjaTw1s4Ob-7BHHAxs2h4GTKK3Q1mJDh9eW9Qb--fvl5d7_efv_2cHe7XVveibLcYJQxSjBmBmZtqxjYQQ7K9Z1jSlKxEbbGlNHWSSaU6YbOsE3fyn5Q1PEb9P7ce0zx91xv06PPyzpmgjhnzRinSgmueEXf_Yce4pymut1CEUm4lKJS7EzZFHNOMOhj8qNJJ02JXozog16M6MWIJkJXI3Xo7aV67kdw_0b-KqjApzMA9S8ePSSdrYfJgvMJbNEu-qf6_wDAb534</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Rahman, Md. Mahfujur</creator><creator>Wie, Jehyung</creator><creator>Cho, Jeong-Hwi</creator><creator>Tae, Hyun-Jin</creator><creator>Ahn, Dongchoon</creator><creator>Lee, Seok Won</creator><creator>Kim, In-Shik</creator><creator>Park, Byung-Yong</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8897-2258</orcidid></search><sort><creationdate>20190601</creationdate><title>Diethylstilbestrol induces morphological changes in the spermatogonia, Sertoli cells and Leydig cells of adult rat</title><author>Rahman, Md. Mahfujur ; Wie, Jehyung ; Cho, Jeong-Hwi ; Tae, Hyun-Jin ; Ahn, Dongchoon ; Lee, Seok Won ; Kim, In-Shik ; Park, Byung-Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-266a9aa9422af2cc592ecf6f9db8d2961474ccc51215d6249a8f8a27b56bf91d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult rat</topic><topic>Apoptosis</topic><topic>Diethylstibestrol</topic><topic>Diethylstilbestrol</topic><topic>Estrogens</topic><topic>Fertility</topic><topic>Labeling</topic><topic>Laboratory animals</topic><topic>Leydig cells</topic><topic>Males</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Reproductive system</topic><topic>Rodents</topic><topic>Sertoli cells</topic><topic>Spermatogenesis</topic><topic>Spermatogonia</topic><topic>Steroidogenesis</topic><topic>Testes</topic><topic>Time dependence</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahman, Md. Mahfujur</creatorcontrib><creatorcontrib>Wie, Jehyung</creatorcontrib><creatorcontrib>Cho, Jeong-Hwi</creatorcontrib><creatorcontrib>Tae, Hyun-Jin</creatorcontrib><creatorcontrib>Ahn, Dongchoon</creatorcontrib><creatorcontrib>Lee, Seok Won</creatorcontrib><creatorcontrib>Kim, In-Shik</creatorcontrib><creatorcontrib>Park, Byung-Yong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahman, Md. Mahfujur</au><au>Wie, Jehyung</au><au>Cho, Jeong-Hwi</au><au>Tae, Hyun-Jin</au><au>Ahn, Dongchoon</au><au>Lee, Seok Won</au><au>Kim, In-Shik</au><au>Park, Byung-Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diethylstilbestrol induces morphological changes in the spermatogonia, Sertoli cells and Leydig cells of adult rat</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>124</volume><spage>433</spage><epage>438</epage><pages>433-438</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>It is now established that diethylstilbestrol (DES) has damaging effects on the male reproductive system. However, to date there have been no studies morphological analysis of adult rat testes upon treatment with DES. Here, we examined whether DES has any significant morphological effect on steroidogenesis and spermatogenesis. DES was injected subcutaneously at 3 μg/day and 30 μg/day in adult male Sprague-Dawley (SD) rats for two different treatment lengths (1 or 3 weeks), after which rats were necropsied. TUNEL labeling, cell counting, and morphological analysis were used to evaluate the effects of DES. A high dose of DES and longer exposure severely affected the cellular development of the testis. Specifically, DES treatment disrupted both steroidogenesis and spermatogenesis by decreasing the number of spermatogonia, Sertoli cells, and Leydig cells in a dose- and time-dependent manner. Thus, DES may account for decreases in the number of spermatogenic cells, Sertoli cells and Leydig cells, which in turn may lead to reduced fertility in males.
•Diethylstilbestrol treatment disrupted both steroidogenesis and spermatogenesis.•Diethylstilbestrol treatment decreased the number of spermatogonia, Sertoli cells, Leydig cells.•Diethylstilbestrol may lead to reduced fertility in males.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31082573</pmid><doi>10.1016/j.rvsc.2019.04.015</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-8897-2258</orcidid></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Adult rat Apoptosis Diethylstibestrol Diethylstilbestrol Estrogens Fertility Labeling Laboratory animals Leydig cells Males Microscopy Morphology Reproductive system Rodents Sertoli cells Spermatogenesis Spermatogonia Steroidogenesis Testes Time dependence Veterinary medicine |
| title | Diethylstilbestrol induces morphological changes in the spermatogonia, Sertoli cells and Leydig cells of adult rat |
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