A novel one-step extracellular staining for flow cytometry: Proof-of-concept on sepsis-related biomarkers

Flow cytometry is a powerful analytical technique. However, it requires time-consuming, multi-step sample procedure. A new protocol was developed to perform extracellular staining and red blood cell lysis in one step, using dry antibodies. Common markers of white blood cells as well as sepsis biomar...

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Veröffentlicht in:	Journal of immunological methods 2019-07, Vol.470, p.59-63
Hauptverfasser:	Bourgoin, Pénélope, Hayman, Jack, Rimmelé, Thomas, Venet, Fabienne, Malergue, Fabrice, Monneret, Guillaume
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Biomarkers - blood Case-Control Studies CD64 Female Flow cytometry Flow Cytometry - methods Gene Expression HLA-DR HLA-DR Antigens - blood HLA-DR Antigens - genetics Humans Male Monocytes - metabolism Monocytes - pathology Neutrophils - metabolism Neutrophils - pathology One-step protocol Receptors, IgG - blood Receptors, IgG - genetics Sepsis Shock, Septic - blood Shock, Septic - diagnosis Shock, Septic - genetics Shock, Septic - mortality Staining and Labeling - methods Survival Analysis Whole blood analysis
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