C-type natriuretic peptide signaling in human follicular environment and its relation with oocyte maturation

C-type natriuretic peptide signaling in human follicular environment and its relation with oocyte maturation

Studies in mice have shown that C-type natriuretic peptide (CNP) is produced by granulosa cells and contributes to ovarian follicle growth and oocyte meiotic arrest until the preovulatory LH surge. In humans, the relationship between intraovarian CNP levels and oocyte meiotic resumption is unknown....

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Veröffentlicht in:Molecular and cellular endocrinology 2019-07, Vol.492, p.110444-110444, Article 110444
Hauptverfasser: Casalechi, Maíra, Dias, Júlia A., Pinto, Lorena V., Lobach, Verônica N., Pereira, Maria T., Cavallo, Ines K., Reis, Adelina M., Dela Cruz, Cynthia, Reis, Fernando M.
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CNP
Follicular fluid
Granulosa cells
IVF
Oocyte maturation
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container_title Molecular and cellular endocrinology
container_volume 492
creator Casalechi, Maíra
Dias, Júlia A.
Pinto, Lorena V.
Lobach, Verônica N.
Pereira, Maria T.
Cavallo, Ines K.
Reis, Adelina M.
Dela Cruz, Cynthia
Reis, Fernando M.
description Studies in mice have shown that C-type natriuretic peptide (CNP) is produced by granulosa cells and contributes to ovarian follicle growth and oocyte meiotic arrest until the preovulatory LH surge. In humans, the relationship between intraovarian CNP levels and oocyte meiotic resumption is unknown. The aim of this study was to investigate whether CNP and its receptor NPR2 are expressed in human ovarian follicles and if their levels change according to the meiotic phase of oocytes. We collected follicular fluid (FF) and luteinized granulosa cells (LGC) from follicle pools (n = 47), and FF, LGC and cumulus cells (CC) from individual follicles (n = 96) during oocyte pickup for in vitro fertilization. There was a positive linear correlation between CNP levels in FF pools and basal antral follicle counting (rs = 0.458; p = 0.002), number of preovulatory follicles >16 mm (rs = 0.361; p = 0.016) and number of oocytes retrieved (rs = 0,378; p = 0.011) and a negative correlation between CNP levels in FF pools and the percentage of mature (MII) oocytes retrieved (rs = −0.39; p = 0.033). FF CNP levels in follicles containing MII oocytes were significantly lower than in follicles containing immature (MI) oocytes (median = 0.44 vs. 0.57 ng/mL, p 
doi_str_mv 10.1016/j.mce.2019.05.003
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In humans, the relationship between intraovarian CNP levels and oocyte meiotic resumption is unknown. The aim of this study was to investigate whether CNP and its receptor NPR2 are expressed in human ovarian follicles and if their levels change according to the meiotic phase of oocytes. We collected follicular fluid (FF) and luteinized granulosa cells (LGC) from follicle pools (n = 47), and FF, LGC and cumulus cells (CC) from individual follicles (n = 96) during oocyte pickup for in vitro fertilization. There was a positive linear correlation between CNP levels in FF pools and basal antral follicle counting (rs = 0.458; p = 0.002), number of preovulatory follicles &gt;16 mm (rs = 0.361; p = 0.016) and number of oocytes retrieved (rs = 0,378; p = 0.011) and a negative correlation between CNP levels in FF pools and the percentage of mature (MII) oocytes retrieved (rs = −0.39; p = 0.033). FF CNP levels in follicles containing MII oocytes were significantly lower than in follicles containing immature (MI) oocytes (median = 0.44 vs. 0.57 ng/mL, p &lt; 0.05). Accordingly, the CNP precursor gene NPPC was 50% less expressed in LGC from follicles containing MII oocytes than in follicles containing MI oocytes (p &lt; 0.01). In addition, NPR2 mRNA was down-regulated in CC surrounding MII oocytes (60% reduction, p &lt; 0.01). CNP signaling is downregulated in human ovarian follicles containing mature oocytes. Further studies should clarify whether CNP signaling is essential to keep oocyte meiotic arrest in humans. •CNP levels in human follicular fluid correlate positively with antral follicle count.•mRNAs for CNP and its receptor NPR2 are expressed in human granulosa cells.•Lower follicular CNP and NPR2 levels are associated with mature oocytes.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2019.05.003</identifier><identifier>PMID: 31075302</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>CNP ; Follicular fluid ; Granulosa cells ; IVF ; Oocyte maturation</subject><ispartof>Molecular and cellular endocrinology, 2019-07, Vol.492, p.110444-110444, Article 110444</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. 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In humans, the relationship between intraovarian CNP levels and oocyte meiotic resumption is unknown. The aim of this study was to investigate whether CNP and its receptor NPR2 are expressed in human ovarian follicles and if their levels change according to the meiotic phase of oocytes. We collected follicular fluid (FF) and luteinized granulosa cells (LGC) from follicle pools (n = 47), and FF, LGC and cumulus cells (CC) from individual follicles (n = 96) during oocyte pickup for in vitro fertilization. There was a positive linear correlation between CNP levels in FF pools and basal antral follicle counting (rs = 0.458; p = 0.002), number of preovulatory follicles &gt;16 mm (rs = 0.361; p = 0.016) and number of oocytes retrieved (rs = 0,378; p = 0.011) and a negative correlation between CNP levels in FF pools and the percentage of mature (MII) oocytes retrieved (rs = −0.39; p = 0.033). FF CNP levels in follicles containing MII oocytes were significantly lower than in follicles containing immature (MI) oocytes (median = 0.44 vs. 0.57 ng/mL, p &lt; 0.05). Accordingly, the CNP precursor gene NPPC was 50% less expressed in LGC from follicles containing MII oocytes than in follicles containing MI oocytes (p &lt; 0.01). In addition, NPR2 mRNA was down-regulated in CC surrounding MII oocytes (60% reduction, p &lt; 0.01). CNP signaling is downregulated in human ovarian follicles containing mature oocytes. Further studies should clarify whether CNP signaling is essential to keep oocyte meiotic arrest in humans. •CNP levels in human follicular fluid correlate positively with antral follicle count.•mRNAs for CNP and its receptor NPR2 are expressed in human granulosa cells.•Lower follicular CNP and NPR2 levels are associated with mature oocytes.</description><subject>CNP</subject><subject>Follicular fluid</subject><subject>Granulosa cells</subject><subject>IVF</subject><subject>Oocyte maturation</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kMFO3DAQhq2KqmyBB-il8pFL0rEdbxJxqlZAKyH1wt1y7Al45djBdkD79g0s7ZHTjEbf_0vzEfKNQc2AbX_s68lgzYH1NcgaQHwiG9a1vOpAtidkAwJE1XJoT8nXnPcA0ErefSGngq2bAL4hfleVw4w06JLckrA4Q2eci7NIs3sI2rvwQF2gj8ukAx2j984sXieK4dmlGCYMhepgqSuZJvS6uBjoiyuPNEZzKEgnXZb0dj4nn0ftM168zzNyf3N9v_tV3f25_b37eVcZIUWpRt51PQDvm2HbD2i3YyNBAw69la0ddDs2GlrLzFYyYVZibBqUg9QjdBI7cUYuj7Vzik8L5qImlw16rwPGJSvOBet5x0CuKDuiJsWcE45qTm7S6aAYqFfHaq9Wx-rVsQKpVsdr5vt7_TJMaP8n_kldgasjgOuPzw6TysZhMGhdQlOUje6D-r81kY7q</recordid><startdate>20190715</startdate><enddate>20190715</enddate><creator>Casalechi, Maíra</creator><creator>Dias, Júlia A.</creator><creator>Pinto, Lorena V.</creator><creator>Lobach, Verônica N.</creator><creator>Pereira, Maria T.</creator><creator>Cavallo, Ines K.</creator><creator>Reis, Adelina M.</creator><creator>Dela Cruz, Cynthia</creator><creator>Reis, Fernando M.</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9258-7472</orcidid><orcidid>https://orcid.org/0000-0003-1444-9290</orcidid></search><sort><creationdate>20190715</creationdate><title>C-type natriuretic peptide signaling in human follicular environment and its relation with oocyte maturation</title><author>Casalechi, Maíra ; Dias, Júlia A. ; Pinto, Lorena V. ; Lobach, Verônica N. ; Pereira, Maria T. ; Cavallo, Ines K. ; Reis, Adelina M. ; Dela Cruz, Cynthia ; Reis, Fernando M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-f288900294b69bed6f450a0eb9d57dba7f4a07d1c6513c9bef44e5b5af085e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>CNP</topic><topic>Follicular fluid</topic><topic>Granulosa cells</topic><topic>IVF</topic><topic>Oocyte maturation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casalechi, Maíra</creatorcontrib><creatorcontrib>Dias, Júlia A.</creatorcontrib><creatorcontrib>Pinto, Lorena V.</creatorcontrib><creatorcontrib>Lobach, Verônica N.</creatorcontrib><creatorcontrib>Pereira, Maria T.</creatorcontrib><creatorcontrib>Cavallo, Ines K.</creatorcontrib><creatorcontrib>Reis, Adelina M.</creatorcontrib><creatorcontrib>Dela Cruz, Cynthia</creatorcontrib><creatorcontrib>Reis, Fernando M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casalechi, Maíra</au><au>Dias, Júlia A.</au><au>Pinto, Lorena V.</au><au>Lobach, Verônica N.</au><au>Pereira, Maria T.</au><au>Cavallo, Ines K.</au><au>Reis, Adelina M.</au><au>Dela Cruz, Cynthia</au><au>Reis, Fernando M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-type natriuretic peptide signaling in human follicular environment and its relation with oocyte maturation</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2019-07-15</date><risdate>2019</risdate><volume>492</volume><spage>110444</spage><epage>110444</epage><pages>110444-110444</pages><artnum>110444</artnum><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Studies in mice have shown that C-type natriuretic peptide (CNP) is produced by granulosa cells and contributes to ovarian follicle growth and oocyte meiotic arrest until the preovulatory LH surge. In humans, the relationship between intraovarian CNP levels and oocyte meiotic resumption is unknown. The aim of this study was to investigate whether CNP and its receptor NPR2 are expressed in human ovarian follicles and if their levels change according to the meiotic phase of oocytes. We collected follicular fluid (FF) and luteinized granulosa cells (LGC) from follicle pools (n = 47), and FF, LGC and cumulus cells (CC) from individual follicles (n = 96) during oocyte pickup for in vitro fertilization. There was a positive linear correlation between CNP levels in FF pools and basal antral follicle counting (rs = 0.458; p = 0.002), number of preovulatory follicles &gt;16 mm (rs = 0.361; p = 0.016) and number of oocytes retrieved (rs = 0,378; p = 0.011) and a negative correlation between CNP levels in FF pools and the percentage of mature (MII) oocytes retrieved (rs = −0.39; p = 0.033). FF CNP levels in follicles containing MII oocytes were significantly lower than in follicles containing immature (MI) oocytes (median = 0.44 vs. 0.57 ng/mL, p &lt; 0.05). Accordingly, the CNP precursor gene NPPC was 50% less expressed in LGC from follicles containing MII oocytes than in follicles containing MI oocytes (p &lt; 0.01). In addition, NPR2 mRNA was down-regulated in CC surrounding MII oocytes (60% reduction, p &lt; 0.01). CNP signaling is downregulated in human ovarian follicles containing mature oocytes. Further studies should clarify whether CNP signaling is essential to keep oocyte meiotic arrest in humans. •CNP levels in human follicular fluid correlate positively with antral follicle count.•mRNAs for CNP and its receptor NPR2 are expressed in human granulosa cells.•Lower follicular CNP and NPR2 levels are associated with mature oocytes.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>31075302</pmid><doi>10.1016/j.mce.2019.05.003</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9258-7472</orcidid><orcidid>https://orcid.org/0000-0003-1444-9290</orcidid></addata></record>
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subjects CNP
Follicular fluid
Granulosa cells
IVF
Oocyte maturation
title C-type natriuretic peptide signaling in human follicular environment and its relation with oocyte maturation
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