Rapid detection of Salmonella in foods using EF-18 agar in conjunction with the hydrophobic grid membrane filter
A new selective and differential culture medium for Salmonella, EF-18 agar, was developed for use with the hydrophobic grid membrane filter (HGMF). The new medium was designed to be both more highly selective and more specific in its differential reactions than its predecessor, selective lysine agar...
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| Veröffentlicht in: | Journal of food protection 1991-12, Vol.54 (12), p.930-934 |
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| container_end_page | 934 |
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| container_issue | 12 |
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| container_title | Journal of food protection |
| container_volume | 54 |
| creator | Entis, P. (QA Life Sciences, Inc., San Diego, CA) Boleszczuk, P |
| description | A new selective and differential culture medium for Salmonella, EF-18 agar, was developed for use with the hydrophobic grid membrane filter (HGMF). The new medium was designed to be both more highly selective and more specific in its differential reactions than its predecessor, selective lysine agar. The HGMF/EF-18 agar method was evaluated against the conventional cultural method (AOAC/BAM) using a total of 954 samples comprising 25 product categories. The HGMF/EF-18 method detected 653 Salmonella-positive samples and the AOAC/BAM method detected 654. The HGMF/EF-18 method with an overall false-negative rate of 2% was determined to be equivalent in sensitivity to the AOAC/BAM procedure. The presumptive false-positive rates were 0.3% for HGMF/EF-18 and 7.9% for AOAC/BAM |
| doi_str_mv | 10.4315/0362-028X-54.12.930 |
| format | Article |
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(QA Life Sciences, Inc., San Diego, CA)</creatorcontrib><creatorcontrib>Boleszczuk, P</creatorcontrib><title>Rapid detection of Salmonella in foods using EF-18 agar in conjunction with the hydrophobic grid membrane filter</title><title>Journal of food protection</title><addtitle>J Food Prot</addtitle><description>A new selective and differential culture medium for Salmonella, EF-18 agar, was developed for use with the hydrophobic grid membrane filter (HGMF). The new medium was designed to be both more highly selective and more specific in its differential reactions than its predecessor, selective lysine agar. The HGMF/EF-18 agar method was evaluated against the conventional cultural method (AOAC/BAM) using a total of 954 samples comprising 25 product categories. The HGMF/EF-18 method detected 653 Salmonella-positive samples and the AOAC/BAM method detected 654. The HGMF/EF-18 method with an overall false-negative rate of 2% was determined to be equivalent in sensitivity to the AOAC/BAM procedure. The presumptive false-positive rates were 0.3% for HGMF/EF-18 and 7.9% for AOAC/BAM</description><subject>AGAR-AGAR</subject><subject>ALIMENTOS</subject><subject>Biological and medical sciences</subject><subject>CONTAMINACION</subject><subject>CONTAMINATION</subject><subject>EXPERIMENTATION EN LABORATOIRE</subject><subject>EXPERIMENTOS EN LABORATORIO</subject><subject>FILTRE</subject><subject>FILTROS</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GELOSE</subject><subject>General aspects</subject><subject>MEMBRANA</subject><subject>MEMBRANE</subject><subject>METHODE</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>METODOS</subject><subject>PRODUIT ALIMENTAIRE</subject><subject>SALMONELLA</subject><issn>0362-028X</issn><issn>1944-9097</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNo9kM1q3DAUhUVJaSZpX6CFokUX3XiqP9vSMgxJUwgUmga6E9f6mVGwLVeyCXn7ysx0VoKr75zL_RD6RMlWcFp_I7xhFWHyT1WLLWVbxckbtKFKiEoR1V6gzZm4RFc5PxNCmGLNO3TJKWmpZGKDpl8wBYutm52ZQxxx9PgR-iGOru8BhxH7GG3GSw7jHt_eVVRi2ENaf0wcn5fxGHsJ8wHPB4cPrzbF6RC7YPA-lerBDV2C0WEf-tml9-ithz67D6f3Gj3d3f7e3VcPP7__2N08VIYrOVc1ENoaYL6TUnjjpFBNZ4VSlINrwNbGtpQQSSgntOMthZY0HjxXtlNQJtfo67F3SvHv4vKsh5DNetTo4pI1Y5xKJSXhBeVH1KSYc3JeTykMkF41JXpVrVeRehWpa6Ep00V1SX0-LVi6wdlz5r_bAnw5AZAN9L5IMCGfuZqKRnFWsI9HzEPUUJRl_fSoSkPNGv4PO8WO4Q</recordid><startdate>19911201</startdate><enddate>19911201</enddate><creator>Entis, P. 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(QA Life Sciences, Inc., San Diego, CA) ; Boleszczuk, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-5a017ca2fb884fce8496bd49913ae6ad5cd7100801301b371a706faf39db9a1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>AGAR-AGAR</topic><topic>ALIMENTOS</topic><topic>Biological and medical sciences</topic><topic>CONTAMINACION</topic><topic>CONTAMINATION</topic><topic>EXPERIMENTATION EN LABORATOIRE</topic><topic>EXPERIMENTOS EN LABORATORIO</topic><topic>FILTRE</topic><topic>FILTROS</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GELOSE</topic><topic>General aspects</topic><topic>MEMBRANA</topic><topic>MEMBRANE</topic><topic>METHODE</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>METODOS</topic><topic>PRODUIT ALIMENTAIRE</topic><topic>SALMONELLA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Entis, P. (QA Life Sciences, Inc., San Diego, CA)</creatorcontrib><creatorcontrib>Boleszczuk, P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of food protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Entis, P. (QA Life Sciences, Inc., San Diego, CA)</au><au>Boleszczuk, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of Salmonella in foods using EF-18 agar in conjunction with the hydrophobic grid membrane filter</atitle><jtitle>Journal of food protection</jtitle><addtitle>J Food Prot</addtitle><date>1991-12-01</date><risdate>1991</risdate><volume>54</volume><issue>12</issue><spage>930</spage><epage>934</epage><pages>930-934</pages><issn>0362-028X</issn><eissn>1944-9097</eissn><coden>JFPRDR</coden><abstract>A new selective and differential culture medium for Salmonella, EF-18 agar, was developed for use with the hydrophobic grid membrane filter (HGMF). The new medium was designed to be both more highly selective and more specific in its differential reactions than its predecessor, selective lysine agar. The HGMF/EF-18 agar method was evaluated against the conventional cultural method (AOAC/BAM) using a total of 954 samples comprising 25 product categories. The HGMF/EF-18 method detected 653 Salmonella-positive samples and the AOAC/BAM method detected 654. The HGMF/EF-18 method with an overall false-negative rate of 2% was determined to be equivalent in sensitivity to the AOAC/BAM procedure. The presumptive false-positive rates were 0.3% for HGMF/EF-18 and 7.9% for AOAC/BAM</abstract><cop>Des Moines, IA</cop><pub>International Association of Milk, Food and Environmental Sanitarians</pub><pmid>31071824</pmid><doi>10.4315/0362-028X-54.12.930</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0362-028X |
| ispartof | Journal of food protection, 1991-12, Vol.54 (12), p.930-934 |
| issn | 0362-028X 1944-9097 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2231898803 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | AGAR-AGAR ALIMENTOS Biological and medical sciences CONTAMINACION CONTAMINATION EXPERIMENTATION EN LABORATOIRE EXPERIMENTOS EN LABORATORIO FILTRE FILTROS Food industries Fundamental and applied biological sciences. Psychology GELOSE General aspects MEMBRANA MEMBRANE METHODE Methods of analysis, processing and quality control, regulation, standards METODOS PRODUIT ALIMENTAIRE SALMONELLA |
| title | Rapid detection of Salmonella in foods using EF-18 agar in conjunction with the hydrophobic grid membrane filter |
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