Scanning laser optical tomography in a neuropathic mouse model: Visualization of structural changes
Background In the field of hearing research a variety of imaging techniques are available to study molecular and cellular structures of the cochlea. Most of them are based on decalcifying, embedding, and cutting of the cochlea. By means of scanning laser optical tomography (SLOT), the complete cochl...
Gespeichert in:
| Veröffentlicht in: | HNO 2019-06, Vol.67 (Suppl 2), p.69-76 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 76 |
|---|---|
| container_issue | Suppl 2 |
| container_start_page | 69 |
| container_title | HNO |
| container_volume | 67 |
| creator | Schulze, J. Nolte, L. Lyutenski, S. Tinne, N. Heinemann, D. Ripken, T. Willaredt, M. A. Nothwang, H. G. Lenarz, T. Warnecke, A. |
| description | Background
In the field of hearing research a variety of imaging techniques are available to study molecular and cellular structures of the cochlea. Most of them are based on decalcifying, embedding, and cutting of the cochlea. By means of scanning laser optical tomography (SLOT), the complete cochlea can be visualized without cutting. The Ca
v
1.3
−/−
mice have already been extensively characterized and show structural changes in the inner ear. Therefore, they were used in this study as a model to investigate whether SLOT can detect structural differences in the murine cochlea.
Materials and methods
Whole undissected cochleae from Ca
v
1.3
−/−
and wild-type mice of various postnatal stages were immunostained and analyzed by SLOT. The results were compared to cochlea preparations that were immunostained and analyzed by fluorescence microscopy. In addition, cochlea preparations were stained with osmium tetraoxide.
Results
Visualization by SLOT showed that the staining of nerve fibers at P27 in Ca
v
1.3
−/−
mice was almost absent compared to wild-type mice and earlier timepoints (P9). The analysis of cochlea preparations confirmed a reduction of the radial nerve fibers. In addition, a significantly reduced number of ribbon synapses per inner hair cell (IHC) at P20 and P27 in the apical part of the cochlea of Ca
v
1.3
−/−
mice was detected.
Conclusion
The visualization of whole non-dissected cochleae by SLOT is a suitable tool for the analysis of gross phenotypic changes, as demonstrated by means of the Ca
v
1.3
−/−
mouse model. For the analysis of finer structures of the cochlea, however, further methods must be used. |
| doi_str_mv | 10.1007/s00106-019-0654-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2231895994</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2231895994</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2112-5ba0e33617c831fd6ca3aa0ac20c6afa25dfd51d84ebb8fbf03cc4b1dc0161b23</originalsourceid><addsrcrecordid>eNp9kE1OwzAQhS0EoqVwADYoSzYGjx07yQqhij-pEgtgbTmO06ZK7GAni96Gs3AyXKWwZDOj0XvzNPMhdAnkBgjJbgMhQAQmUGAieIrpEZpDyhgmKc-P0TzKGRZQ0Bk6C2EbR15QdopmLK7xTNA5unvTytrGrpNWBeMT1w-NVm0yuM6tveo3u6Sxifr-smb0rlfDptFJ58ZgYq1Me45OatUGc3HoC_Tx-PC-fMar16eX5f0KawpAMS8VMYwJyHTOoK6EVkwpojQlWqhaUV7VFYcqT01Z5nVZE6Z1WkKlCQgoKVug6ym39-5zNGGQXRO0aVtlTbxGUsogL3hRpNEKk1V7F4I3tex90ym_k0DknpucuMnITe65yX381SF-LDtT_W38gooGOhlClOzaeLl1o7fx5X9SfwD-c3mF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2231895994</pqid></control><display><type>article</type><title>Scanning laser optical tomography in a neuropathic mouse model: Visualization of structural changes</title><source>SpringerLink Journals - AutoHoldings</source><creator>Schulze, J. ; Nolte, L. ; Lyutenski, S. ; Tinne, N. ; Heinemann, D. ; Ripken, T. ; Willaredt, M. A. ; Nothwang, H. G. ; Lenarz, T. ; Warnecke, A.</creator><creatorcontrib>Schulze, J. ; Nolte, L. ; Lyutenski, S. ; Tinne, N. ; Heinemann, D. ; Ripken, T. ; Willaredt, M. A. ; Nothwang, H. G. ; Lenarz, T. ; Warnecke, A.</creatorcontrib><description>Background
In the field of hearing research a variety of imaging techniques are available to study molecular and cellular structures of the cochlea. Most of them are based on decalcifying, embedding, and cutting of the cochlea. By means of scanning laser optical tomography (SLOT), the complete cochlea can be visualized without cutting. The Ca
v
1.3
−/−
mice have already been extensively characterized and show structural changes in the inner ear. Therefore, they were used in this study as a model to investigate whether SLOT can detect structural differences in the murine cochlea.
Materials and methods
Whole undissected cochleae from Ca
v
1.3
−/−
and wild-type mice of various postnatal stages were immunostained and analyzed by SLOT. The results were compared to cochlea preparations that were immunostained and analyzed by fluorescence microscopy. In addition, cochlea preparations were stained with osmium tetraoxide.
Results
Visualization by SLOT showed that the staining of nerve fibers at P27 in Ca
v
1.3
−/−
mice was almost absent compared to wild-type mice and earlier timepoints (P9). The analysis of cochlea preparations confirmed a reduction of the radial nerve fibers. In addition, a significantly reduced number of ribbon synapses per inner hair cell (IHC) at P20 and P27 in the apical part of the cochlea of Ca
v
1.3
−/−
mice was detected.
Conclusion
The visualization of whole non-dissected cochleae by SLOT is a suitable tool for the analysis of gross phenotypic changes, as demonstrated by means of the Ca
v
1.3
−/−
mouse model. For the analysis of finer structures of the cochlea, however, further methods must be used.</description><identifier>ISSN: 0017-6192</identifier><identifier>EISSN: 1433-0458</identifier><identifier>DOI: 10.1007/s00106-019-0654-2</identifier><identifier>PMID: 31065762</identifier><language>eng</language><publisher>Munich: Springer Medizin</publisher><subject>Allergology ; Head and Neck Surgery ; Medicine ; Medicine & Public Health ; Original Articles ; Otorhinolaryngology ; Plastic Surgery</subject><ispartof>HNO, 2019-06, Vol.67 (Suppl 2), p.69-76</ispartof><rights>Springer Medizin Verlag GmbH, ein Teil von Springer Nature 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2112-5ba0e33617c831fd6ca3aa0ac20c6afa25dfd51d84ebb8fbf03cc4b1dc0161b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00106-019-0654-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00106-019-0654-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31065762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schulze, J.</creatorcontrib><creatorcontrib>Nolte, L.</creatorcontrib><creatorcontrib>Lyutenski, S.</creatorcontrib><creatorcontrib>Tinne, N.</creatorcontrib><creatorcontrib>Heinemann, D.</creatorcontrib><creatorcontrib>Ripken, T.</creatorcontrib><creatorcontrib>Willaredt, M. A.</creatorcontrib><creatorcontrib>Nothwang, H. G.</creatorcontrib><creatorcontrib>Lenarz, T.</creatorcontrib><creatorcontrib>Warnecke, A.</creatorcontrib><title>Scanning laser optical tomography in a neuropathic mouse model: Visualization of structural changes</title><title>HNO</title><addtitle>HNO</addtitle><addtitle>HNO</addtitle><description>Background
In the field of hearing research a variety of imaging techniques are available to study molecular and cellular structures of the cochlea. Most of them are based on decalcifying, embedding, and cutting of the cochlea. By means of scanning laser optical tomography (SLOT), the complete cochlea can be visualized without cutting. The Ca
v
1.3
−/−
mice have already been extensively characterized and show structural changes in the inner ear. Therefore, they were used in this study as a model to investigate whether SLOT can detect structural differences in the murine cochlea.
Materials and methods
Whole undissected cochleae from Ca
v
1.3
−/−
and wild-type mice of various postnatal stages were immunostained and analyzed by SLOT. The results were compared to cochlea preparations that were immunostained and analyzed by fluorescence microscopy. In addition, cochlea preparations were stained with osmium tetraoxide.
Results
Visualization by SLOT showed that the staining of nerve fibers at P27 in Ca
v
1.3
−/−
mice was almost absent compared to wild-type mice and earlier timepoints (P9). The analysis of cochlea preparations confirmed a reduction of the radial nerve fibers. In addition, a significantly reduced number of ribbon synapses per inner hair cell (IHC) at P20 and P27 in the apical part of the cochlea of Ca
v
1.3
−/−
mice was detected.
Conclusion
The visualization of whole non-dissected cochleae by SLOT is a suitable tool for the analysis of gross phenotypic changes, as demonstrated by means of the Ca
v
1.3
−/−
mouse model. For the analysis of finer structures of the cochlea, however, further methods must be used.</description><subject>Allergology</subject><subject>Head and Neck Surgery</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Articles</subject><subject>Otorhinolaryngology</subject><subject>Plastic Surgery</subject><issn>0017-6192</issn><issn>1433-0458</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kE1OwzAQhS0EoqVwADYoSzYGjx07yQqhij-pEgtgbTmO06ZK7GAni96Gs3AyXKWwZDOj0XvzNPMhdAnkBgjJbgMhQAQmUGAieIrpEZpDyhgmKc-P0TzKGRZQ0Bk6C2EbR15QdopmLK7xTNA5unvTytrGrpNWBeMT1w-NVm0yuM6tveo3u6Sxifr-smb0rlfDptFJ58ZgYq1Me45OatUGc3HoC_Tx-PC-fMar16eX5f0KawpAMS8VMYwJyHTOoK6EVkwpojQlWqhaUV7VFYcqT01Z5nVZE6Z1WkKlCQgoKVug6ym39-5zNGGQXRO0aVtlTbxGUsogL3hRpNEKk1V7F4I3tex90ym_k0DknpucuMnITe65yX381SF-LDtT_W38gooGOhlClOzaeLl1o7fx5X9SfwD-c3mF</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Schulze, J.</creator><creator>Nolte, L.</creator><creator>Lyutenski, S.</creator><creator>Tinne, N.</creator><creator>Heinemann, D.</creator><creator>Ripken, T.</creator><creator>Willaredt, M. A.</creator><creator>Nothwang, H. G.</creator><creator>Lenarz, T.</creator><creator>Warnecke, A.</creator><general>Springer Medizin</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190601</creationdate><title>Scanning laser optical tomography in a neuropathic mouse model</title><author>Schulze, J. ; Nolte, L. ; Lyutenski, S. ; Tinne, N. ; Heinemann, D. ; Ripken, T. ; Willaredt, M. A. ; Nothwang, H. G. ; Lenarz, T. ; Warnecke, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2112-5ba0e33617c831fd6ca3aa0ac20c6afa25dfd51d84ebb8fbf03cc4b1dc0161b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Allergology</topic><topic>Head and Neck Surgery</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Articles</topic><topic>Otorhinolaryngology</topic><topic>Plastic Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schulze, J.</creatorcontrib><creatorcontrib>Nolte, L.</creatorcontrib><creatorcontrib>Lyutenski, S.</creatorcontrib><creatorcontrib>Tinne, N.</creatorcontrib><creatorcontrib>Heinemann, D.</creatorcontrib><creatorcontrib>Ripken, T.</creatorcontrib><creatorcontrib>Willaredt, M. A.</creatorcontrib><creatorcontrib>Nothwang, H. G.</creatorcontrib><creatorcontrib>Lenarz, T.</creatorcontrib><creatorcontrib>Warnecke, A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>HNO</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schulze, J.</au><au>Nolte, L.</au><au>Lyutenski, S.</au><au>Tinne, N.</au><au>Heinemann, D.</au><au>Ripken, T.</au><au>Willaredt, M. A.</au><au>Nothwang, H. G.</au><au>Lenarz, T.</au><au>Warnecke, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scanning laser optical tomography in a neuropathic mouse model: Visualization of structural changes</atitle><jtitle>HNO</jtitle><stitle>HNO</stitle><addtitle>HNO</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>67</volume><issue>Suppl 2</issue><spage>69</spage><epage>76</epage><pages>69-76</pages><issn>0017-6192</issn><eissn>1433-0458</eissn><abstract>Background
In the field of hearing research a variety of imaging techniques are available to study molecular and cellular structures of the cochlea. Most of them are based on decalcifying, embedding, and cutting of the cochlea. By means of scanning laser optical tomography (SLOT), the complete cochlea can be visualized without cutting. The Ca
v
1.3
−/−
mice have already been extensively characterized and show structural changes in the inner ear. Therefore, they were used in this study as a model to investigate whether SLOT can detect structural differences in the murine cochlea.
Materials and methods
Whole undissected cochleae from Ca
v
1.3
−/−
and wild-type mice of various postnatal stages were immunostained and analyzed by SLOT. The results were compared to cochlea preparations that were immunostained and analyzed by fluorescence microscopy. In addition, cochlea preparations were stained with osmium tetraoxide.
Results
Visualization by SLOT showed that the staining of nerve fibers at P27 in Ca
v
1.3
−/−
mice was almost absent compared to wild-type mice and earlier timepoints (P9). The analysis of cochlea preparations confirmed a reduction of the radial nerve fibers. In addition, a significantly reduced number of ribbon synapses per inner hair cell (IHC) at P20 and P27 in the apical part of the cochlea of Ca
v
1.3
−/−
mice was detected.
Conclusion
The visualization of whole non-dissected cochleae by SLOT is a suitable tool for the analysis of gross phenotypic changes, as demonstrated by means of the Ca
v
1.3
−/−
mouse model. For the analysis of finer structures of the cochlea, however, further methods must be used.</abstract><cop>Munich</cop><pub>Springer Medizin</pub><pmid>31065762</pmid><doi>10.1007/s00106-019-0654-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0017-6192 |
| ispartof | HNO, 2019-06, Vol.67 (Suppl 2), p.69-76 |
| issn | 0017-6192 1433-0458 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2231895994 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Allergology Head and Neck Surgery Medicine Medicine & Public Health Original Articles Otorhinolaryngology Plastic Surgery |
| title | Scanning laser optical tomography in a neuropathic mouse model: Visualization of structural changes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T12%3A01%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Scanning%20laser%20optical%20tomography%20in%20a%C2%A0neuropathic%20mouse%20model:%20Visualization%20of%20structural%20changes&rft.jtitle=HNO&rft.au=Schulze,%20J.&rft.date=2019-06-01&rft.volume=67&rft.issue=Suppl%202&rft.spage=69&rft.epage=76&rft.pages=69-76&rft.issn=0017-6192&rft.eissn=1433-0458&rft_id=info:doi/10.1007/s00106-019-0654-2&rft_dat=%3Cproquest_cross%3E2231895994%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2231895994&rft_id=info:pmid/31065762&rfr_iscdi=true |