Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecC in wild rabbits
Aims To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates. Methods and Results Nasal and rectal swabs of 103 mammals were collected in Aragón during the...
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| Veröffentlicht in: | Journal of applied microbiology 2019-07, Vol.127 (1), p.284-291 |
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| creator | Ruiz‐Ripa, L. Alcalá, L. Simón, C. Gómez, P. Mama, O.M. Rezusta, A. Zarazaga, M. Torres, C. |
| description | Aims
To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates.
Methods and Results
Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012–2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin‐resistant S. aureus (MRSA). Three MRSA were mecC‐positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA‐carrying isolate from European hedgehog, typed as ST1‐t386‐SCCmecIVa‐agrIII and it harboured the blaZ, erm(C), ant(6)‐Ia and aph(3´)‐IIIa resistance genes. A high diversity of spa‐types was detected among the 19 methicillin‐susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found.
Conclusions
Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC‐MRSA.
Significance and Impact of the Study
This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain. |
| doi_str_mv | 10.1111/jam.14301 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2231889326</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2235933989</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-e113b01584a71c0b75b3c8d90117e4e323d2538e900d5c1abe18c0ffe5816fb13</originalsourceid><addsrcrecordid>eNp1kc1O3DAURq2qVYGhi75AZambViLg6zvOz3I0tNAKVImh68hxbopHSTy1E9Cs6CPwjDwJnhnKolK9-Xylo3Mtf4y9B3EM8ZwsdXcMUxTwiu0DpiqRaSZfb-_TRIlM7rGDEJZCAAqVvmV7CCLFVOI-uz-1t-SDHdbcNXwx6NXNunXGGTMGrkdPMUzregrc9vzOtjXvdNfpdjvPvP7l-iO-WGkb484ON7ymgcxgXb8RXl4tZnxxHfc-_nnoyMxfLF5XlR3CIXvTRBm9e84J-_n1y_X8PLn4cfZtPrtIDCqEhACwEqDyqc7AiCpTFZq8LgRARlNCibVUmFMhRK0M6IogN6JpSOWQNhXghH3aeVfe_R4pDGVng6G21T25MZRSIuR5gTKN6Md_0KUbfR9ft6FUgVhEcMI-7yjjXQiemnLlbaf9ugRRblopYyvltpXIfng2jlVH9Qv5t4YInOyA-DW0_r-p_D673CmfAGPrlWw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2235933989</pqid></control><display><type>article</type><title>Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecC in wild rabbits</title><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Ruiz‐Ripa, L. ; Alcalá, L. ; Simón, C. ; Gómez, P. ; Mama, O.M. ; Rezusta, A. ; Zarazaga, M. ; Torres, C.</creator><creatorcontrib>Ruiz‐Ripa, L. ; Alcalá, L. ; Simón, C. ; Gómez, P. ; Mama, O.M. ; Rezusta, A. ; Zarazaga, M. ; Torres, C.</creatorcontrib><description>Aims
To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates.
Methods and Results
Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012–2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin‐resistant S. aureus (MRSA). Three MRSA were mecC‐positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA‐carrying isolate from European hedgehog, typed as ST1‐t386‐SCCmecIVa‐agrIII and it harboured the blaZ, erm(C), ant(6)‐Ia and aph(3´)‐IIIa resistance genes. A high diversity of spa‐types was detected among the 19 methicillin‐susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found.
Conclusions
Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC‐MRSA.
Significance and Impact of the Study
This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14301</identifier><identifier>PMID: 31063623</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Antiinfectives and antibacterials ; Antimicrobial agents ; Antimicrobial resistance ; CC130 ; Drug resistance ; free‐living animals ; Genes ; Genotypes ; Mammals ; mecA‐MRSA ; mecC‐MRSA ; Methicillin ; Pathogens ; Penicillin ; Phenotypes ; Rabbits ; Rectum ; Resistance factors ; Staphylococcal enterotoxin F ; Staphylococcus aureus ; Staphylococcus infections ; Virulence ; Virulence factors</subject><ispartof>Journal of applied microbiology, 2019-07, Vol.127 (1), p.284-291</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-e113b01584a71c0b75b3c8d90117e4e323d2538e900d5c1abe18c0ffe5816fb13</citedby><cites>FETCH-LOGICAL-c3531-e113b01584a71c0b75b3c8d90117e4e323d2538e900d5c1abe18c0ffe5816fb13</cites><orcidid>0000-0003-3709-1690 ; 0000-0002-8703-5489</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14301$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14301$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31063623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruiz‐Ripa, L.</creatorcontrib><creatorcontrib>Alcalá, L.</creatorcontrib><creatorcontrib>Simón, C.</creatorcontrib><creatorcontrib>Gómez, P.</creatorcontrib><creatorcontrib>Mama, O.M.</creatorcontrib><creatorcontrib>Rezusta, A.</creatorcontrib><creatorcontrib>Zarazaga, M.</creatorcontrib><creatorcontrib>Torres, C.</creatorcontrib><title>Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecC in wild rabbits</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates.
Methods and Results
Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012–2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin‐resistant S. aureus (MRSA). Three MRSA were mecC‐positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA‐carrying isolate from European hedgehog, typed as ST1‐t386‐SCCmecIVa‐agrIII and it harboured the blaZ, erm(C), ant(6)‐Ia and aph(3´)‐IIIa resistance genes. A high diversity of spa‐types was detected among the 19 methicillin‐susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found.
Conclusions
Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC‐MRSA.
Significance and Impact of the Study
This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain.</description><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>CC130</subject><subject>Drug resistance</subject><subject>free‐living animals</subject><subject>Genes</subject><subject>Genotypes</subject><subject>Mammals</subject><subject>mecA‐MRSA</subject><subject>mecC‐MRSA</subject><subject>Methicillin</subject><subject>Pathogens</subject><subject>Penicillin</subject><subject>Phenotypes</subject><subject>Rabbits</subject><subject>Rectum</subject><subject>Resistance factors</subject><subject>Staphylococcal enterotoxin F</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kc1O3DAURq2qVYGhi75AZambViLg6zvOz3I0tNAKVImh68hxbopHSTy1E9Cs6CPwjDwJnhnKolK9-Xylo3Mtf4y9B3EM8ZwsdXcMUxTwiu0DpiqRaSZfb-_TRIlM7rGDEJZCAAqVvmV7CCLFVOI-uz-1t-SDHdbcNXwx6NXNunXGGTMGrkdPMUzregrc9vzOtjXvdNfpdjvPvP7l-iO-WGkb484ON7ymgcxgXb8RXl4tZnxxHfc-_nnoyMxfLF5XlR3CIXvTRBm9e84J-_n1y_X8PLn4cfZtPrtIDCqEhACwEqDyqc7AiCpTFZq8LgRARlNCibVUmFMhRK0M6IogN6JpSOWQNhXghH3aeVfe_R4pDGVng6G21T25MZRSIuR5gTKN6Md_0KUbfR9ft6FUgVhEcMI-7yjjXQiemnLlbaf9ugRRblopYyvltpXIfng2jlVH9Qv5t4YInOyA-DW0_r-p_D673CmfAGPrlWw</recordid><startdate>201907</startdate><enddate>201907</enddate><creator>Ruiz‐Ripa, L.</creator><creator>Alcalá, L.</creator><creator>Simón, C.</creator><creator>Gómez, P.</creator><creator>Mama, O.M.</creator><creator>Rezusta, A.</creator><creator>Zarazaga, M.</creator><creator>Torres, C.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3709-1690</orcidid><orcidid>https://orcid.org/0000-0002-8703-5489</orcidid></search><sort><creationdate>201907</creationdate><title>Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecC in wild rabbits</title><author>Ruiz‐Ripa, L. ; Alcalá, L. ; Simón, C. ; Gómez, P. ; Mama, O.M. ; Rezusta, A. ; Zarazaga, M. ; Torres, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-e113b01584a71c0b75b3c8d90117e4e323d2538e900d5c1abe18c0ffe5816fb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>CC130</topic><topic>Drug resistance</topic><topic>free‐living animals</topic><topic>Genes</topic><topic>Genotypes</topic><topic>Mammals</topic><topic>mecA‐MRSA</topic><topic>mecC‐MRSA</topic><topic>Methicillin</topic><topic>Pathogens</topic><topic>Penicillin</topic><topic>Phenotypes</topic><topic>Rabbits</topic><topic>Rectum</topic><topic>Resistance factors</topic><topic>Staphylococcal enterotoxin F</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus infections</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruiz‐Ripa, L.</creatorcontrib><creatorcontrib>Alcalá, L.</creatorcontrib><creatorcontrib>Simón, C.</creatorcontrib><creatorcontrib>Gómez, P.</creatorcontrib><creatorcontrib>Mama, O.M.</creatorcontrib><creatorcontrib>Rezusta, A.</creatorcontrib><creatorcontrib>Zarazaga, M.</creatorcontrib><creatorcontrib>Torres, C.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruiz‐Ripa, L.</au><au>Alcalá, L.</au><au>Simón, C.</au><au>Gómez, P.</au><au>Mama, O.M.</au><au>Rezusta, A.</au><au>Zarazaga, M.</au><au>Torres, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecC in wild rabbits</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-07</date><risdate>2019</risdate><volume>127</volume><issue>1</issue><spage>284</spage><epage>291</epage><pages>284-291</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates.
Methods and Results
Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012–2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin‐resistant S. aureus (MRSA). Three MRSA were mecC‐positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA‐carrying isolate from European hedgehog, typed as ST1‐t386‐SCCmecIVa‐agrIII and it harboured the blaZ, erm(C), ant(6)‐Ia and aph(3´)‐IIIa resistance genes. A high diversity of spa‐types was detected among the 19 methicillin‐susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found.
Conclusions
Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC‐MRSA.
Significance and Impact of the Study
This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31063623</pmid><doi>10.1111/jam.14301</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3709-1690</orcidid><orcidid>https://orcid.org/0000-0002-8703-5489</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2019-07, Vol.127 (1), p.284-291 |
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| source | Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Antiinfectives and antibacterials Antimicrobial agents Antimicrobial resistance CC130 Drug resistance free‐living animals Genes Genotypes Mammals mecA‐MRSA mecC‐MRSA Methicillin Pathogens Penicillin Phenotypes Rabbits Rectum Resistance factors Staphylococcal enterotoxin F Staphylococcus aureus Staphylococcus infections Virulence Virulence factors |
| title | Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130‐mecC in wild rabbits |
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