Comparison of the immobilization of lipase from Pseudomonas fluorescens on divinylsulfone or p-benzoquinone activated support

NiZnFe2O4 superparamagnetic nanoparticles were coated with silica by impregnation with tetraethoxysilane (TEOS) and further activated with divinylsulfone (DVS) and p-benzoquinone (BQ) for covalent immobilization lipase from Pseudomonas fluorescens (PFL), producing the biocatalysts TEOS-NANO-DVS-PFL...

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Veröffentlicht in:	International journal of biological macromolecules 2019-08, Vol.134, p.936-945
Hauptverfasser:	Rios, Nathalia Saraiva, Neto, Davino M. Andrade, dos Santos, José Cleiton Sousa, Fechine, Pierre Basílio Almeida, Fernández-Lafuente, Roberto, Gonçalves, Luciana Rocha Barros
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Benzoquinones - chemistry Covalent binding Enzyme immobilization Enzyme Stability Enzyme stabilization Enzymes, Immobilized Hydrogen-Ion Concentration Lipase Lipase - chemistry Metal Nanoparticles - chemistry Models, Molecular Protein Conformation Pseudomonas fluorescens - enzymology Spectroscopy, Fourier Transform Infrared Sulfones - chemistry Thermodynamics
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