In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota
Our previous study has proved that the three polysaccharide fractions from L. japonica (LP-A4, LP-A6, and LP-A8) had significantly different structure characterization. Herein, we conducted in vitro simulated digestion and fermentation to study the digestive mechanism of LP-As. The results of gastro...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2019-07, Vol.67 (26), p.7496-7505 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7505 |
|---|---|
| container_issue | 26 |
| container_start_page | 7496 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 67 |
| creator | Gao, Jie Lin, Lianzhu Chen, Zijie Cai, Yongjian Xiao, Chuqiao Zhou, Feibai Sun, Baoguo Zhao, Mouming |
| description | Our previous study has proved that the three polysaccharide fractions from L. japonica (LP-A4, LP-A6, and LP-A8) had significantly different structure characterization. Herein, we conducted in vitro simulated digestion and fermentation to study the digestive mechanism of LP-As. The results of gastrointestinal digestion indicated that LP-A6 and LP-A8 would be easier to trap the enzyme molecules for their denser interconnected macromolecule network compared with LP-A4. Fermentation of LP-As by human gut microbiota, especially for LP-A8, generated a large amount of short-chain fatty acids (SCFAs), which could upregulate the abundance of Firmicutes (Lachnoclostridium and Eubacterium). The high content of sulfate and highly branched sugar residue of LP-A8 might help it be easily used by Firmicutes in gut microbiota of hyperlipidemic patients. Functional analysis revealed that the increased metabolic activities of glycerophospholipid metabolism, ether lipid metabolism, and fatty acid metabolism induced by LP-A8 treatment were closely associated with metabolic syndromes and hyperlipidemia. |
| doi_str_mv | 10.1021/acs.jafc.9b00970 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2231850389</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2231850389</sourcerecordid><originalsourceid>FETCH-LOGICAL-a336t-e4d01e192833019a2680ec2325f19ab54169e13c8a7f5a2134f1a925e14f7233</originalsourceid><addsrcrecordid>eNp1kb1u2zAURomiQeOm3TsVHDtULi8pytIYpHFiwEE6GF2FK-qqpiGKKikNeZ0-aeifdutEkDzfwSU_xj6BWIKQ8A1NXB6wM8uqEaJaiTdsAVqKTAOUb9lCJCYrdQHX7H2MByFEqVfiHbtWADJXhV6wP5uB_7RT8Py7_UVxsn7gOLR8TcHRMOHpwHd8tw9E_IfvXyIas8dgW-LrgOYIRN4F7_gWnR3SDfIDjn6wBk-q3Z5s4Bs3Jpgn29aOtuVPNGHjextddhujNxYnavnj7HDgD_PEn6wJvrF-wg_sqsM-0sfLesN26_vd3WO2fX7Y3N1uM1SqmDLKWwEElSyVElChLEpBRiqpu7RrdA5FRaBMiatOowSVd4CV1AR5t5JK3bAvZ-0Y_O85fUXtbDTU9ziQn2MtpYJSC1VWCRVnNI0YY6CuHoN1GF5qEPWxmDoVUx-LqS_FpMjni31uHLX_An-bSMDXM3CK-jkM6a3_970CeJmbAg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2231850389</pqid></control><display><type>article</type><title>In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota</title><source>MEDLINE</source><source>American Chemical Society (ACS) Journals</source><creator>Gao, Jie ; Lin, Lianzhu ; Chen, Zijie ; Cai, Yongjian ; Xiao, Chuqiao ; Zhou, Feibai ; Sun, Baoguo ; Zhao, Mouming</creator><creatorcontrib>Gao, Jie ; Lin, Lianzhu ; Chen, Zijie ; Cai, Yongjian ; Xiao, Chuqiao ; Zhou, Feibai ; Sun, Baoguo ; Zhao, Mouming</creatorcontrib><description>Our previous study has proved that the three polysaccharide fractions from L. japonica (LP-A4, LP-A6, and LP-A8) had significantly different structure characterization. Herein, we conducted in vitro simulated digestion and fermentation to study the digestive mechanism of LP-As. The results of gastrointestinal digestion indicated that LP-A6 and LP-A8 would be easier to trap the enzyme molecules for their denser interconnected macromolecule network compared with LP-A4. Fermentation of LP-As by human gut microbiota, especially for LP-A8, generated a large amount of short-chain fatty acids (SCFAs), which could upregulate the abundance of Firmicutes (Lachnoclostridium and Eubacterium). The high content of sulfate and highly branched sugar residue of LP-A8 might help it be easily used by Firmicutes in gut microbiota of hyperlipidemic patients. Functional analysis revealed that the increased metabolic activities of glycerophospholipid metabolism, ether lipid metabolism, and fatty acid metabolism induced by LP-A8 treatment were closely associated with metabolic syndromes and hyperlipidemia.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.9b00970</identifier><identifier>PMID: 31124365</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bacteria - classification ; Bacteria - drug effects ; Bacteria - genetics ; Bacteria - metabolism ; Digestion ; Fatty Acids, Volatile - metabolism ; Feces - microbiology ; Female ; Fermentation ; Gastrointestinal Microbiome - drug effects ; Humans ; Laminaria - chemistry ; Lipid Metabolism - drug effects ; Male ; Middle Aged ; Phylogeny ; Plant Extracts - pharmacology ; Polysaccharides - pharmacology</subject><ispartof>Journal of agricultural and food chemistry, 2019-07, Vol.67 (26), p.7496-7505</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-e4d01e192833019a2680ec2325f19ab54169e13c8a7f5a2134f1a925e14f7233</citedby><cites>FETCH-LOGICAL-a336t-e4d01e192833019a2680ec2325f19ab54169e13c8a7f5a2134f1a925e14f7233</cites><orcidid>0000-0003-4657-1231 ; 0000-0003-0221-3838 ; 0000-0003-4326-8237</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.9b00970$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.9b00970$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31124365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Jie</creatorcontrib><creatorcontrib>Lin, Lianzhu</creatorcontrib><creatorcontrib>Chen, Zijie</creatorcontrib><creatorcontrib>Cai, Yongjian</creatorcontrib><creatorcontrib>Xiao, Chuqiao</creatorcontrib><creatorcontrib>Zhou, Feibai</creatorcontrib><creatorcontrib>Sun, Baoguo</creatorcontrib><creatorcontrib>Zhao, Mouming</creatorcontrib><title>In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Our previous study has proved that the three polysaccharide fractions from L. japonica (LP-A4, LP-A6, and LP-A8) had significantly different structure characterization. Herein, we conducted in vitro simulated digestion and fermentation to study the digestive mechanism of LP-As. The results of gastrointestinal digestion indicated that LP-A6 and LP-A8 would be easier to trap the enzyme molecules for their denser interconnected macromolecule network compared with LP-A4. Fermentation of LP-As by human gut microbiota, especially for LP-A8, generated a large amount of short-chain fatty acids (SCFAs), which could upregulate the abundance of Firmicutes (Lachnoclostridium and Eubacterium). The high content of sulfate and highly branched sugar residue of LP-A8 might help it be easily used by Firmicutes in gut microbiota of hyperlipidemic patients. Functional analysis revealed that the increased metabolic activities of glycerophospholipid metabolism, ether lipid metabolism, and fatty acid metabolism induced by LP-A8 treatment were closely associated with metabolic syndromes and hyperlipidemia.</description><subject>Bacteria - classification</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Digestion</subject><subject>Fatty Acids, Volatile - metabolism</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Fermentation</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Humans</subject><subject>Laminaria - chemistry</subject><subject>Lipid Metabolism - drug effects</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Phylogeny</subject><subject>Plant Extracts - pharmacology</subject><subject>Polysaccharides - pharmacology</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kb1u2zAURomiQeOm3TsVHDtULi8pytIYpHFiwEE6GF2FK-qqpiGKKikNeZ0-aeifdutEkDzfwSU_xj6BWIKQ8A1NXB6wM8uqEaJaiTdsAVqKTAOUb9lCJCYrdQHX7H2MByFEqVfiHbtWADJXhV6wP5uB_7RT8Py7_UVxsn7gOLR8TcHRMOHpwHd8tw9E_IfvXyIas8dgW-LrgOYIRN4F7_gWnR3SDfIDjn6wBk-q3Z5s4Bs3Jpgn29aOtuVPNGHjextddhujNxYnavnj7HDgD_PEn6wJvrF-wg_sqsM-0sfLesN26_vd3WO2fX7Y3N1uM1SqmDLKWwEElSyVElChLEpBRiqpu7RrdA5FRaBMiatOowSVd4CV1AR5t5JK3bAvZ-0Y_O85fUXtbDTU9ziQn2MtpYJSC1VWCRVnNI0YY6CuHoN1GF5qEPWxmDoVUx-LqS_FpMjni31uHLX_An-bSMDXM3CK-jkM6a3_970CeJmbAg</recordid><startdate>20190703</startdate><enddate>20190703</enddate><creator>Gao, Jie</creator><creator>Lin, Lianzhu</creator><creator>Chen, Zijie</creator><creator>Cai, Yongjian</creator><creator>Xiao, Chuqiao</creator><creator>Zhou, Feibai</creator><creator>Sun, Baoguo</creator><creator>Zhao, Mouming</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4657-1231</orcidid><orcidid>https://orcid.org/0000-0003-0221-3838</orcidid><orcidid>https://orcid.org/0000-0003-4326-8237</orcidid></search><sort><creationdate>20190703</creationdate><title>In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota</title><author>Gao, Jie ; Lin, Lianzhu ; Chen, Zijie ; Cai, Yongjian ; Xiao, Chuqiao ; Zhou, Feibai ; Sun, Baoguo ; Zhao, Mouming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-e4d01e192833019a2680ec2325f19ab54169e13c8a7f5a2134f1a925e14f7233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacteria - classification</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Digestion</topic><topic>Fatty Acids, Volatile - metabolism</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Fermentation</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>Humans</topic><topic>Laminaria - chemistry</topic><topic>Lipid Metabolism - drug effects</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Phylogeny</topic><topic>Plant Extracts - pharmacology</topic><topic>Polysaccharides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Jie</creatorcontrib><creatorcontrib>Lin, Lianzhu</creatorcontrib><creatorcontrib>Chen, Zijie</creatorcontrib><creatorcontrib>Cai, Yongjian</creatorcontrib><creatorcontrib>Xiao, Chuqiao</creatorcontrib><creatorcontrib>Zhou, Feibai</creatorcontrib><creatorcontrib>Sun, Baoguo</creatorcontrib><creatorcontrib>Zhao, Mouming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Jie</au><au>Lin, Lianzhu</au><au>Chen, Zijie</au><au>Cai, Yongjian</au><au>Xiao, Chuqiao</au><au>Zhou, Feibai</au><au>Sun, Baoguo</au><au>Zhao, Mouming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2019-07-03</date><risdate>2019</risdate><volume>67</volume><issue>26</issue><spage>7496</spage><epage>7505</epage><pages>7496-7505</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Our previous study has proved that the three polysaccharide fractions from L. japonica (LP-A4, LP-A6, and LP-A8) had significantly different structure characterization. Herein, we conducted in vitro simulated digestion and fermentation to study the digestive mechanism of LP-As. The results of gastrointestinal digestion indicated that LP-A6 and LP-A8 would be easier to trap the enzyme molecules for their denser interconnected macromolecule network compared with LP-A4. Fermentation of LP-As by human gut microbiota, especially for LP-A8, generated a large amount of short-chain fatty acids (SCFAs), which could upregulate the abundance of Firmicutes (Lachnoclostridium and Eubacterium). The high content of sulfate and highly branched sugar residue of LP-A8 might help it be easily used by Firmicutes in gut microbiota of hyperlipidemic patients. Functional analysis revealed that the increased metabolic activities of glycerophospholipid metabolism, ether lipid metabolism, and fatty acid metabolism induced by LP-A8 treatment were closely associated with metabolic syndromes and hyperlipidemia.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>31124365</pmid><doi>10.1021/acs.jafc.9b00970</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4657-1231</orcidid><orcidid>https://orcid.org/0000-0003-0221-3838</orcidid><orcidid>https://orcid.org/0000-0003-4326-8237</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2019-07, Vol.67 (26), p.7496-7505 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2231850389 |
| source | MEDLINE; American Chemical Society (ACS) Journals |
| subjects | Bacteria - classification Bacteria - drug effects Bacteria - genetics Bacteria - metabolism Digestion Fatty Acids, Volatile - metabolism Feces - microbiology Female Fermentation Gastrointestinal Microbiome - drug effects Humans Laminaria - chemistry Lipid Metabolism - drug effects Male Middle Aged Phylogeny Plant Extracts - pharmacology Polysaccharides - pharmacology |
| title | In Vitro Digestion and Fermentation of Three Polysaccharide Fractions from Laminaria japonica and Their Impact on Lipid Metabolism-Associated Human Gut Microbiota |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T14%3A03%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20Vitro%20Digestion%20and%20Fermentation%20of%20Three%20Polysaccharide%20Fractions%20from%20Laminaria%20japonica%20and%20Their%20Impact%20on%20Lipid%20Metabolism-Associated%20Human%20Gut%20Microbiota&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Gao,%20Jie&rft.date=2019-07-03&rft.volume=67&rft.issue=26&rft.spage=7496&rft.epage=7505&rft.pages=7496-7505&rft.issn=0021-8561&rft.eissn=1520-5118&rft_id=info:doi/10.1021/acs.jafc.9b00970&rft_dat=%3Cproquest_cross%3E2231850389%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2231850389&rft_id=info:pmid/31124365&rfr_iscdi=true |