Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry
Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the othe...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2019-06, Vol.91 (12), p.7738-7743 |
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| creator | Szarka, Máte Szigeti, Márton Guttman, András |
| description | Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15% and 6.79%, respectively. |
| doi_str_mv | 10.1021/acs.analchem.9b01028 |
| format | Article |
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Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. 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Chem</addtitle><description>Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15% and 6.79%, respectively.</description><subject>Capillary electrophoresis</subject><subject>Carbohydrates</subject><subject>Chemistry</subject><subject>Electrophoresis</subject><subject>Electrospraying</subject><subject>Fluorescence</subject><subject>Glycan</subject><subject>Ionization</subject><subject>Ions</subject><subject>Laser induced fluorescence</subject><subject>Lasers</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Polysaccharides</subject><subject>Quantitation</subject><subject>Quantitative analysis</subject><subject>Scientific imaging</subject><subject>Spectroscopy</subject><subject>Sugar</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv2zAQhIkgRey6_QdFQSCXXOQuX3ocC9duDLjoob4LFLV0FEiiS0oH99eHfh5y6InA7jdDzA4hXxjMGXD2TZsw171uzQt286KCOMzvyJQpDkma5_yeTAFAJDwDmJCPIbwCMAYsfSATwZhImcympF13etf0O7rRAX2y7uvRYE1X7eg8BoO9QfoDBzRD43qqBzq8IN3qQ-s8XbgeqbN02ca1d2Hv9YGuXd_80yf6lw6B_tmflh0O_vCJfLC6Dfj58s7IdrXcLp6Tze-f68X3TaIlE0NiK1UVSklZMyuNVQUXNkWwNUcEgYYrwavcoAKdizRjMaFEtHWqRK4yLmbk6Wy79-7viGEouyZGaVvdoxtDyblguSx4XkT08R366kYfr3qkZMqVyk6UPFMmpgwebbn3Taf9oWRQHssoYxnltYzyUkaUfb2Yj1WH9U10vX4E4Awc5beP_-v5BtaimT4</recordid><startdate>20190618</startdate><enddate>20190618</enddate><creator>Szarka, Máte</creator><creator>Szigeti, Márton</creator><creator>Guttman, András</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2670-5554</orcidid></search><sort><creationdate>20190618</creationdate><title>Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry</title><author>Szarka, Máte ; Szigeti, Márton ; Guttman, András</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a413t-fb5b95544d1f4cf5923f6e0fd2ee03ec2532b8ce50a836712704eefd65385723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Capillary electrophoresis</topic><topic>Carbohydrates</topic><topic>Chemistry</topic><topic>Electrophoresis</topic><topic>Electrospraying</topic><topic>Fluorescence</topic><topic>Glycan</topic><topic>Ionization</topic><topic>Ions</topic><topic>Laser induced fluorescence</topic><topic>Lasers</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Polysaccharides</topic><topic>Quantitation</topic><topic>Quantitative analysis</topic><topic>Scientific imaging</topic><topic>Spectroscopy</topic><topic>Sugar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szarka, Máte</creatorcontrib><creatorcontrib>Szigeti, Márton</creatorcontrib><creatorcontrib>Guttman, András</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szarka, Máte</au><au>Szigeti, Márton</au><au>Guttman, András</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2019-06-18</date><risdate>2019</risdate><volume>91</volume><issue>12</issue><spage>7738</spage><epage>7743</epage><pages>7738-7743</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. 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| subjects | Capillary electrophoresis Carbohydrates Chemistry Electrophoresis Electrospraying Fluorescence Glycan Ionization Ions Laser induced fluorescence Lasers Mass spectrometry Mass spectroscopy Polysaccharides Quantitation Quantitative analysis Scientific imaging Spectroscopy Sugar |
| title | Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry |
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