Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development
Present study analyses the co-localisation of RIP5 with FGFR1, FGFR2 and HIP2 in the developing kidney, as RIP5 is a major determinant of urinary tract development, downstream of FGF-signaling. Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were anal...
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| Veröffentlicht in: | Acta histochemica 2019-07, Vol.121 (5), p.531-538 |
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| creator | Racetin, Anita Raguž, Fila Durdov, Merica Glavina Kunac, Nenad Saraga, Marijan Sanna-Cherchi, Simone Šoljić, Violeta Martinović, Vlatka Petričević, Joško Kostić, Sandra Mardešić, Snježana Tomaš, Sandra Zekić Kablar, Boris Restović, Ivana Lozić, Mirela Filipović, Natalija Saraga-Babić, Mirna Vukojević, Katarina |
| description | Present study analyses the co-localisation of RIP5 with FGFR1, FGFR2 and HIP2 in the developing kidney, as RIP5 is a major determinant of urinary tract development, downstream of FGF-signaling.
Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were analysed using double-immunofluorescence method with RIP5/FGFR1/FGFR2 and HIP2 markers. Quantification of positive cells were performed using Kruskal–Wallis test.
In the 6th week of kidney development RIP5 (89.6%) and HIP2 (39.6%) are strongly expressed in the metanephric mesenchyme. FGFR1 shows moderate/strong expression in the developing nephrons (87.3%) and collecting ducts (70.5%) (p |
| doi_str_mv | 10.1016/j.acthis.2019.04.011 |
| format | Article |
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Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were analysed using double-immunofluorescence method with RIP5/FGFR1/FGFR2 and HIP2 markers. Quantification of positive cells were performed using Kruskal–Wallis test.
In the 6th week of kidney development RIP5 (89.6%) and HIP2 (39.6%) are strongly expressed in the metanephric mesenchyme. FGFR1 shows moderate/strong expression in the developing nephrons (87.3%) and collecting ducts (70.5%) (p < 0.05). RIP5/FGFR1 co-localized at the marginal zone and the ureteric bud with predominant FGFR1 expression. FGFR2 (26.1%) shows similar expression pattern as FGFR1 (70.5%) in the same kidney structures. RIP5/FGFR2 co-localized at the marginal zone and the collecting ducts (predominant expression of FGFR2). HIP2 is strongly expressed in collecting ducts (96.7%), and co-localized with RIP5. In 10th week, RIP5 expression decrease (74.2%), while the pattern of expression of RIP5 and FGFR1 in collecting ducts (33.4% and 91.9%) and developing nephrons (21.9% and 32.4%) (p < 0.05) is similar to that in the 6th developmental week. Ureter is moderately expressing RIP5 while FGFR1 is strongly expressed in the ureteric wall. FGFR2 is strongly expressed in the collecting ducts (84.3%) and ureter. HIP2 have 81.1% positive cells in the collecting duct. RIP5/FGFR1 co-localize in collecting ducts and Henley’s loop.
The expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the human kidney development might indicate their important roles in metanephric development and ureteric muscle layer differentiation through FGF signaling pathways.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1016/j.acthis.2019.04.011</identifier><identifier>PMID: 31047684</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>FGFR1 ; FGFR2 ; Human embryo ; Kidney development ; RIP5</subject><ispartof>Acta histochemica, 2019-07, Vol.121 (5), p.531-538</ispartof><rights>2019 Elsevier GmbH</rights><rights>Copyright © 2019 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-f65c11944b5d339d3110ebca668e34b27630d4414d61be4bf3f242252fb396ed3</citedby><cites>FETCH-LOGICAL-c362t-f65c11944b5d339d3110ebca668e34b27630d4414d61be4bf3f242252fb396ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0065128119300297$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31047684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Racetin, Anita</creatorcontrib><creatorcontrib>Raguž, Fila</creatorcontrib><creatorcontrib>Durdov, Merica Glavina</creatorcontrib><creatorcontrib>Kunac, Nenad</creatorcontrib><creatorcontrib>Saraga, Marijan</creatorcontrib><creatorcontrib>Sanna-Cherchi, Simone</creatorcontrib><creatorcontrib>Šoljić, Violeta</creatorcontrib><creatorcontrib>Martinović, Vlatka</creatorcontrib><creatorcontrib>Petričević, Joško</creatorcontrib><creatorcontrib>Kostić, Sandra</creatorcontrib><creatorcontrib>Mardešić, Snježana</creatorcontrib><creatorcontrib>Tomaš, Sandra Zekić</creatorcontrib><creatorcontrib>Kablar, Boris</creatorcontrib><creatorcontrib>Restović, Ivana</creatorcontrib><creatorcontrib>Lozić, Mirela</creatorcontrib><creatorcontrib>Filipović, Natalija</creatorcontrib><creatorcontrib>Saraga-Babić, Mirna</creatorcontrib><creatorcontrib>Vukojević, Katarina</creatorcontrib><title>Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>Present study analyses the co-localisation of RIP5 with FGFR1, FGFR2 and HIP2 in the developing kidney, as RIP5 is a major determinant of urinary tract development, downstream of FGF-signaling.
Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were analysed using double-immunofluorescence method with RIP5/FGFR1/FGFR2 and HIP2 markers. Quantification of positive cells were performed using Kruskal–Wallis test.
In the 6th week of kidney development RIP5 (89.6%) and HIP2 (39.6%) are strongly expressed in the metanephric mesenchyme. FGFR1 shows moderate/strong expression in the developing nephrons (87.3%) and collecting ducts (70.5%) (p < 0.05). RIP5/FGFR1 co-localized at the marginal zone and the ureteric bud with predominant FGFR1 expression. FGFR2 (26.1%) shows similar expression pattern as FGFR1 (70.5%) in the same kidney structures. RIP5/FGFR2 co-localized at the marginal zone and the collecting ducts (predominant expression of FGFR2). HIP2 is strongly expressed in collecting ducts (96.7%), and co-localized with RIP5. In 10th week, RIP5 expression decrease (74.2%), while the pattern of expression of RIP5 and FGFR1 in collecting ducts (33.4% and 91.9%) and developing nephrons (21.9% and 32.4%) (p < 0.05) is similar to that in the 6th developmental week. Ureter is moderately expressing RIP5 while FGFR1 is strongly expressed in the ureteric wall. FGFR2 is strongly expressed in the collecting ducts (84.3%) and ureter. HIP2 have 81.1% positive cells in the collecting duct. RIP5/FGFR1 co-localize in collecting ducts and Henley’s loop.
The expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the human kidney development might indicate their important roles in metanephric development and ureteric muscle layer differentiation through FGF signaling pathways.</description><subject>FGFR1</subject><subject>FGFR2</subject><subject>Human embryo</subject><subject>Kidney development</subject><subject>RIP5</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kMFq3DAURUVpaaZJ_qAULbuIXT1J1tibQgmZZCDQENq1kKVnRlNbciU7JH8fB6dZdvU2597LO4R8BlYCA_XtWBo7HXwuOYOmZLJkAO_IBhTUBRNb_p5sGFNVAbyGE_Ip5yNjrGGCfyQnApjcqlpuSL8fhjnEpWeK9oCDt6an-DgmzNnHQEczTZgCjR29399VF3R3vbuH9XBqgqM3-ztOfaDTAWmIaVjyh3kwgf7xLuATdfiAfRwHDNMZ-dCZPuP56z0lv3dXvy5vituf1_vLH7eFFYpPRacqC9BI2VZOiMYJAIatNUrVKGTLt0owJyVIp6BF2Xai45LzinetaBQ6cUq-rr1jin9nzJMefLbY9yZgnLPmnDdc1GwLCypX1KaYc8JOj8kPJj1pYPrFsz7q1bN-8ayZ1IvnJfbldWFuB3RvoX9iF-D7CuDy54PHpLP1GCw6n9BO2kX__4VnWeCOvg</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Racetin, Anita</creator><creator>Raguž, Fila</creator><creator>Durdov, Merica Glavina</creator><creator>Kunac, Nenad</creator><creator>Saraga, Marijan</creator><creator>Sanna-Cherchi, Simone</creator><creator>Šoljić, Violeta</creator><creator>Martinović, Vlatka</creator><creator>Petričević, Joško</creator><creator>Kostić, Sandra</creator><creator>Mardešić, Snježana</creator><creator>Tomaš, Sandra Zekić</creator><creator>Kablar, Boris</creator><creator>Restović, Ivana</creator><creator>Lozić, Mirela</creator><creator>Filipović, Natalija</creator><creator>Saraga-Babić, Mirna</creator><creator>Vukojević, Katarina</creator><general>Elsevier GmbH</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190701</creationdate><title>Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development</title><author>Racetin, Anita ; Raguž, Fila ; Durdov, Merica Glavina ; Kunac, Nenad ; Saraga, Marijan ; Sanna-Cherchi, Simone ; Šoljić, Violeta ; Martinović, Vlatka ; Petričević, Joško ; Kostić, Sandra ; Mardešić, Snježana ; Tomaš, Sandra Zekić ; Kablar, Boris ; Restović, Ivana ; Lozić, Mirela ; Filipović, Natalija ; Saraga-Babić, Mirna ; Vukojević, Katarina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-f65c11944b5d339d3110ebca668e34b27630d4414d61be4bf3f242252fb396ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>FGFR1</topic><topic>FGFR2</topic><topic>Human embryo</topic><topic>Kidney development</topic><topic>RIP5</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Racetin, Anita</creatorcontrib><creatorcontrib>Raguž, Fila</creatorcontrib><creatorcontrib>Durdov, Merica Glavina</creatorcontrib><creatorcontrib>Kunac, Nenad</creatorcontrib><creatorcontrib>Saraga, Marijan</creatorcontrib><creatorcontrib>Sanna-Cherchi, Simone</creatorcontrib><creatorcontrib>Šoljić, Violeta</creatorcontrib><creatorcontrib>Martinović, Vlatka</creatorcontrib><creatorcontrib>Petričević, Joško</creatorcontrib><creatorcontrib>Kostić, Sandra</creatorcontrib><creatorcontrib>Mardešić, Snježana</creatorcontrib><creatorcontrib>Tomaš, Sandra Zekić</creatorcontrib><creatorcontrib>Kablar, Boris</creatorcontrib><creatorcontrib>Restović, Ivana</creatorcontrib><creatorcontrib>Lozić, Mirela</creatorcontrib><creatorcontrib>Filipović, Natalija</creatorcontrib><creatorcontrib>Saraga-Babić, Mirna</creatorcontrib><creatorcontrib>Vukojević, Katarina</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Racetin, Anita</au><au>Raguž, Fila</au><au>Durdov, Merica Glavina</au><au>Kunac, Nenad</au><au>Saraga, Marijan</au><au>Sanna-Cherchi, Simone</au><au>Šoljić, Violeta</au><au>Martinović, Vlatka</au><au>Petričević, Joško</au><au>Kostić, Sandra</au><au>Mardešić, Snježana</au><au>Tomaš, Sandra Zekić</au><au>Kablar, Boris</au><au>Restović, Ivana</au><au>Lozić, Mirela</au><au>Filipović, Natalija</au><au>Saraga-Babić, Mirna</au><au>Vukojević, Katarina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>121</volume><issue>5</issue><spage>531</spage><epage>538</epage><pages>531-538</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>Present study analyses the co-localisation of RIP5 with FGFR1, FGFR2 and HIP2 in the developing kidney, as RIP5 is a major determinant of urinary tract development, downstream of FGF-signaling.
Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were analysed using double-immunofluorescence method with RIP5/FGFR1/FGFR2 and HIP2 markers. Quantification of positive cells were performed using Kruskal–Wallis test.
In the 6th week of kidney development RIP5 (89.6%) and HIP2 (39.6%) are strongly expressed in the metanephric mesenchyme. FGFR1 shows moderate/strong expression in the developing nephrons (87.3%) and collecting ducts (70.5%) (p < 0.05). RIP5/FGFR1 co-localized at the marginal zone and the ureteric bud with predominant FGFR1 expression. FGFR2 (26.1%) shows similar expression pattern as FGFR1 (70.5%) in the same kidney structures. RIP5/FGFR2 co-localized at the marginal zone and the collecting ducts (predominant expression of FGFR2). HIP2 is strongly expressed in collecting ducts (96.7%), and co-localized with RIP5. In 10th week, RIP5 expression decrease (74.2%), while the pattern of expression of RIP5 and FGFR1 in collecting ducts (33.4% and 91.9%) and developing nephrons (21.9% and 32.4%) (p < 0.05) is similar to that in the 6th developmental week. Ureter is moderately expressing RIP5 while FGFR1 is strongly expressed in the ureteric wall. FGFR2 is strongly expressed in the collecting ducts (84.3%) and ureter. HIP2 have 81.1% positive cells in the collecting duct. RIP5/FGFR1 co-localize in collecting ducts and Henley’s loop.
The expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the human kidney development might indicate their important roles in metanephric development and ureteric muscle layer differentiation through FGF signaling pathways.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>31047684</pmid><doi>10.1016/j.acthis.2019.04.011</doi><tpages>8</tpages></addata></record> |
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| subjects | FGFR1 FGFR2 Human embryo Kidney development RIP5 |
| title | Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development |
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