Establishment, characterization and plant regeneration from highly chlorophyllous embryogenic cell cultures of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. ex Steud
A finely dispersed, homogeneous and highly chlorophyllous cell suspension (TIANSJ98 cell line) was obtained from shoot apices of Bouteloua gracilis (H.B.K.) Lag. ex Steud. cultured on MPC medium containing MS salts supplemented with 2,4-D (1 mg/l), BAP (2 mg/l) and adenine (40 mg/l). When the TIANSJ...
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| Veröffentlicht in: | Plant cell reports 2001-02, Vol.20 (2), p.131-136 |
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| creator | Aguado Santacruz, GA Cabrera Ponce, JL Ramirez Chavez, E Leon Ramirez, CG Rascon Cruz, Q Herrera Estrella, L Olalde-Portugal, V |
| description | A finely dispersed, homogeneous and highly chlorophyllous cell suspension (TIANSJ98 cell line) was obtained from shoot apices of Bouteloua gracilis (H.B.K.) Lag. ex Steud. cultured on MPC medium containing MS salts supplemented with 2,4-D (1 mg/l), BAP (2 mg/l) and adenine (40 mg/l). When the TIANSJ98 cell line was grown in this medium with shaking at 180 rpm it had doubling times of 7.2 and 3.7 days in terms of fresh and dry weight, respectively. Total chlorophyll content in this cell culture ranged from 121.6 to 18.3 μg/g FW at 12 and 21 days following culture initiation. Plants regenerated from the TIANSJ98 cell line, via somatic embryogenesis, were grown to maturity and produced seeds. Although different cell culture systems have been described for cereals and grasses, to the best of our knowledge this is the first report of a highly chlorophyllous and regenerable cell suspension in Poaceae. |
| doi_str_mv | 10.1007/s002990000293 |
| format | Article |
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Lag. ex Steud</title><source>SpringerLink Journals - AutoHoldings</source><creator>Aguado Santacruz, GA ; Cabrera Ponce, JL ; Ramirez Chavez, E ; Leon Ramirez, CG ; Rascon Cruz, Q ; Herrera Estrella, L ; Olalde-Portugal, V</creator><creatorcontrib>Aguado Santacruz, GA ; Cabrera Ponce, JL ; Ramirez Chavez, E ; Leon Ramirez, CG ; Rascon Cruz, Q ; Herrera Estrella, L ; Olalde-Portugal, V</creatorcontrib><description>A finely dispersed, homogeneous and highly chlorophyllous cell suspension (TIANSJ98 cell line) was obtained from shoot apices of Bouteloua gracilis (H.B.K.) Lag. ex Steud. cultured on MPC medium containing MS salts supplemented with 2,4-D (1 mg/l), BAP (2 mg/l) and adenine (40 mg/l). When the TIANSJ98 cell line was grown in this medium with shaking at 180 rpm it had doubling times of 7.2 and 3.7 days in terms of fresh and dry weight, respectively. Total chlorophyll content in this cell culture ranged from 121.6 to 18.3 μg/g FW at 12 and 21 days following culture initiation. Plants regenerated from the TIANSJ98 cell line, via somatic embryogenesis, were grown to maturity and produced seeds. Although different cell culture systems have been described for cereals and grasses, to the best of our knowledge this is the first report of a highly chlorophyllous and regenerable cell suspension in Poaceae.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990000293</identifier><identifier>PMID: 30759899</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Biological and medical sciences ; Biotechnology ; Bouteloua gracilis ; Cells ; Embryonic growth stage ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Grasses ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; Methods. Procedures. 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Lag. ex Steud</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>A finely dispersed, homogeneous and highly chlorophyllous cell suspension (TIANSJ98 cell line) was obtained from shoot apices of Bouteloua gracilis (H.B.K.) Lag. ex Steud. cultured on MPC medium containing MS salts supplemented with 2,4-D (1 mg/l), BAP (2 mg/l) and adenine (40 mg/l). When the TIANSJ98 cell line was grown in this medium with shaking at 180 rpm it had doubling times of 7.2 and 3.7 days in terms of fresh and dry weight, respectively. Total chlorophyll content in this cell culture ranged from 121.6 to 18.3 μg/g FW at 12 and 21 days following culture initiation. Plants regenerated from the TIANSJ98 cell line, via somatic embryogenesis, were grown to maturity and produced seeds. Although different cell culture systems have been described for cereals and grasses, to the best of our knowledge this is the first report of a highly chlorophyllous and regenerable cell suspension in Poaceae.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bouteloua gracilis</subject><subject>Cells</subject><subject>Embryonic growth stage</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Grasses</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>Methods. Procedures. 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Lag. ex Steud</title><author>Aguado Santacruz, GA ; Cabrera Ponce, JL ; Ramirez Chavez, E ; Leon Ramirez, CG ; Rascon Cruz, Q ; Herrera Estrella, L ; Olalde-Portugal, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-20990b528a293c238630a0387c14556ce1052a15fde44bbffb00868eca1b46ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Bouteloua gracilis</topic><topic>Cells</topic><topic>Embryonic growth stage</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Grasses</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>Methods. Procedures. Technologies</topic><topic>Plant cells and fungal cells</topic><topic>Poaceae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aguado Santacruz, GA</creatorcontrib><creatorcontrib>Cabrera Ponce, JL</creatorcontrib><creatorcontrib>Ramirez Chavez, E</creatorcontrib><creatorcontrib>Leon Ramirez, CG</creatorcontrib><creatorcontrib>Rascon Cruz, Q</creatorcontrib><creatorcontrib>Herrera Estrella, L</creatorcontrib><creatorcontrib>Olalde-Portugal, V</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aguado Santacruz, GA</au><au>Cabrera Ponce, JL</au><au>Ramirez Chavez, E</au><au>Leon Ramirez, CG</au><au>Rascon Cruz, Q</au><au>Herrera Estrella, L</au><au>Olalde-Portugal, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment, characterization and plant regeneration from highly chlorophyllous embryogenic cell cultures of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. ex Steud</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>20</volume><issue>2</issue><spage>131</spage><epage>136</epage><pages>131-136</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>A finely dispersed, homogeneous and highly chlorophyllous cell suspension (TIANSJ98 cell line) was obtained from shoot apices of Bouteloua gracilis (H.B.K.) Lag. ex Steud. cultured on MPC medium containing MS salts supplemented with 2,4-D (1 mg/l), BAP (2 mg/l) and adenine (40 mg/l). When the TIANSJ98 cell line was grown in this medium with shaking at 180 rpm it had doubling times of 7.2 and 3.7 days in terms of fresh and dry weight, respectively. Total chlorophyll content in this cell culture ranged from 121.6 to 18.3 μg/g FW at 12 and 21 days following culture initiation. Plants regenerated from the TIANSJ98 cell line, via somatic embryogenesis, were grown to maturity and produced seeds. Although different cell culture systems have been described for cereals and grasses, to the best of our knowledge this is the first report of a highly chlorophyllous and regenerable cell suspension in Poaceae.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>30759899</pmid><doi>10.1007/s002990000293</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Biotechnology Bouteloua gracilis Cells Embryonic growth stage Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Grasses In vitro propagation: entire plant regeneration from tissues and cell cultures Methods. Procedures. Technologies Plant cells and fungal cells Poaceae |
| title | Establishment, characterization and plant regeneration from highly chlorophyllous embryogenic cell cultures of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. ex Steud |
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