Phytoplasmas Associated with Grapevine Yellows Disease in Chile
An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction...
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| Veröffentlicht in: | Plant disease 2009-08, Vol.93 (8), p.789-796 |
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| creator | Gajardo, A Fiore, N Prodan, S Botti, S Paltrinieri, S Pino, A.M Zamorano, A Montealegre, J Bertaccini, A |
| description | An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses, cloning, and sequencing allowed identification of phytoplasmas belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A, and 16SrXII-A. The 16SrVII-A phytoplasma represents a new finding in grapevine; moreover, variability of the RFLP profile was observed in some of the 16SrXII-A phytoplasmas, indicating possible new ribosomal subgroups. Mixed phytoplasma infections and infections of phytoplasmas together with one or more viruses also occurred. |
| doi_str_mv | 10.1094/PDIS-93-8-0789 |
| format | Article |
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Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses, cloning, and sequencing allowed identification of phytoplasmas belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A, and 16SrXII-A. The 16SrVII-A phytoplasma represents a new finding in grapevine; moreover, variability of the RFLP profile was observed in some of the 16SrXII-A phytoplasmas, indicating possible new ribosomal subgroups. Mixed phytoplasma infections and infections of phytoplasmas together with one or more viruses also occurred.</description><identifier>ISSN: 0191-2917</identifier><identifier>EISSN: 1943-7692</identifier><identifier>DOI: 10.1094/PDIS-93-8-0789</identifier><identifier>PMID: 30764324</identifier><identifier>CODEN: PLDIDE</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>16SrI (Aster yellows group) ; 16SrVII (Ash yellows group) ; 16SrXII (Stolbur group) ; Bacterial plant pathogens ; Biological and medical sciences ; disease detection ; disease surveillance ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control ; grapes ; mixed infection ; molecular sequence data ; new host records ; nucleotide sequences ; pathogen identification ; Phytopathology. Animal pests. Plant and forest protection ; phytoplasmal diseases ; plant pathogenic bacteria ; plant viruses ; restriction fragment length polymorphism ; Vitaceae ; Vitis vinifera</subject><ispartof>Plant disease, 2009-08, Vol.93 (8), p.789-796</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-3ee7a4cbbc841ff17f273ec92844553e27fad97b0b27d4321315a933ee765f053</citedby><cites>FETCH-LOGICAL-c420t-3ee7a4cbbc841ff17f273ec92844553e27fad97b0b27d4321315a933ee765f053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3722,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21770913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30764324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gajardo, A</creatorcontrib><creatorcontrib>Fiore, N</creatorcontrib><creatorcontrib>Prodan, S</creatorcontrib><creatorcontrib>Botti, S</creatorcontrib><creatorcontrib>Paltrinieri, S</creatorcontrib><creatorcontrib>Pino, A.M</creatorcontrib><creatorcontrib>Zamorano, A</creatorcontrib><creatorcontrib>Montealegre, J</creatorcontrib><creatorcontrib>Bertaccini, A</creatorcontrib><title>Phytoplasmas Associated with Grapevine Yellows Disease in Chile</title><title>Plant disease</title><addtitle>Plant Dis</addtitle><description>An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses, cloning, and sequencing allowed identification of phytoplasmas belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A, and 16SrXII-A. The 16SrVII-A phytoplasma represents a new finding in grapevine; moreover, variability of the RFLP profile was observed in some of the 16SrXII-A phytoplasmas, indicating possible new ribosomal subgroups. Mixed phytoplasma infections and infections of phytoplasmas together with one or more viruses also occurred.</description><subject>16SrI (Aster yellows group)</subject><subject>16SrVII (Ash yellows group)</subject><subject>16SrXII (Stolbur group)</subject><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>disease detection</subject><subject>disease surveillance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</subject><subject>grapes</subject><subject>mixed infection</subject><subject>molecular sequence data</subject><subject>new host records</subject><subject>nucleotide sequences</subject><subject>pathogen identification</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>phytoplasmal diseases</subject><subject>plant pathogenic bacteria</subject><subject>plant viruses</subject><subject>restriction fragment length polymorphism</subject><subject>Vitaceae</subject><subject>Vitis vinifera</subject><issn>0191-2917</issn><issn>1943-7692</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp90E1LAzEQBuAgitbq1aPuRfGydfKxm81JpH4VCgrag6eQTRMb2XbrztbSf29Ka4_CwFyedxheQs4o9CgocfN6P3hLFU-LFGSh9kiHKsFTmSu2TzpAFU2ZovKIHCN-AYAQeXFIjjjIXHAmOuT2dbJq63llcGowuUOsbTCtGyfL0E6Sp8bM3U-YueTDVVW9xOQ-oDPokjBL-pNQuRNy4E2F7nS7u2T0-PDef06HL0-D_t0wtYJBm3LnpBG2LG0hqPdUeia5s4oVQmQZd0x6M1ayhJLJcfyMcpoZxdexPPOQ8S653tydN_X3wmGrpwFtfMrMXL1AzRhToAopZKRX_1OQQIGpCHsbaJsasXFez5swNc1KU9DrdvW6Xa24LvS63Rg4315elFM33vG_OiO43AKD1lS-MTMbcOcYlRIU5dFdbJw3tTafTTSjNwaUA80LFof_AuEXifA</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Gajardo, A</creator><creator>Fiore, N</creator><creator>Prodan, S</creator><creator>Botti, S</creator><creator>Paltrinieri, S</creator><creator>Pino, A.M</creator><creator>Zamorano, A</creator><creator>Montealegre, J</creator><creator>Bertaccini, A</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>Phytoplasmas Associated with Grapevine Yellows Disease in Chile</title><author>Gajardo, A ; Fiore, N ; Prodan, S ; Botti, S ; Paltrinieri, S ; Pino, A.M ; Zamorano, A ; Montealegre, J ; Bertaccini, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-3ee7a4cbbc841ff17f273ec92844553e27fad97b0b27d4321315a933ee765f053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>16SrI (Aster yellows group)</topic><topic>16SrVII (Ash yellows group)</topic><topic>16SrXII (Stolbur group)</topic><topic>Bacterial plant pathogens</topic><topic>Biological and medical sciences</topic><topic>disease detection</topic><topic>disease surveillance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</topic><topic>grapes</topic><topic>mixed infection</topic><topic>molecular sequence data</topic><topic>new host records</topic><topic>nucleotide sequences</topic><topic>pathogen identification</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>phytoplasmal diseases</topic><topic>plant pathogenic bacteria</topic><topic>plant viruses</topic><topic>restriction fragment length polymorphism</topic><topic>Vitaceae</topic><topic>Vitis vinifera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gajardo, A</creatorcontrib><creatorcontrib>Fiore, N</creatorcontrib><creatorcontrib>Prodan, S</creatorcontrib><creatorcontrib>Botti, S</creatorcontrib><creatorcontrib>Paltrinieri, S</creatorcontrib><creatorcontrib>Pino, A.M</creatorcontrib><creatorcontrib>Zamorano, A</creatorcontrib><creatorcontrib>Montealegre, J</creatorcontrib><creatorcontrib>Bertaccini, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gajardo, A</au><au>Fiore, N</au><au>Prodan, S</au><au>Botti, S</au><au>Paltrinieri, S</au><au>Pino, A.M</au><au>Zamorano, A</au><au>Montealegre, J</au><au>Bertaccini, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytoplasmas Associated with Grapevine Yellows Disease in Chile</atitle><jtitle>Plant disease</jtitle><addtitle>Plant Dis</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>93</volume><issue>8</issue><spage>789</spage><epage>796</epage><pages>789-796</pages><issn>0191-2917</issn><eissn>1943-7692</eissn><coden>PLDIDE</coden><abstract>An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses, cloning, and sequencing allowed identification of phytoplasmas belonging to ribosomal subgroups 16SrI-B, 16SrI-C, 16SrVII-A, and 16SrXII-A. The 16SrVII-A phytoplasma represents a new finding in grapevine; moreover, variability of the RFLP profile was observed in some of the 16SrXII-A phytoplasmas, indicating possible new ribosomal subgroups. Mixed phytoplasma infections and infections of phytoplasmas together with one or more viruses also occurred.</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><pmid>30764324</pmid><doi>10.1094/PDIS-93-8-0789</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | 16SrI (Aster yellows group) 16SrVII (Ash yellows group) 16SrXII (Stolbur group) Bacterial plant pathogens Biological and medical sciences disease detection disease surveillance Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control grapes mixed infection molecular sequence data new host records nucleotide sequences pathogen identification Phytopathology. Animal pests. Plant and forest protection phytoplasmal diseases plant pathogenic bacteria plant viruses restriction fragment length polymorphism Vitaceae Vitis vinifera |
| title | Phytoplasmas Associated with Grapevine Yellows Disease in Chile |
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