Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results
The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes....
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| Veröffentlicht in: | Lasers in medical science 2019-08, Vol.34 (6), p.1253-1260 |
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| creator | Filipini, Silvana Martins Rodrigues Campagnolo, Cibele Bruno Dutra, Danilo Antônio Milbradt Maciel, Roberto Marinho Danesi, Cristiane Cadermatori Kantorski, Karla Zanini |
| description | The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment,
n
= 5), SRPG (SRP,
n
= 10), aPDTW (SRP+aPDT-MB/water,
n
= 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose,
n
= 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (
p
0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics. |
| doi_str_mv | 10.1007/s10103-019-02733-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2229092042</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2177442944</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-cafa668ed9a42c67773a2553b8f61ae539e2c26ccacf3af886ed253007d958e43</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi0EokPhBVggS2zYhPoWO2FXVVCQKrEBiZ3lOCcdjxI7-FIxj8Lb4nQKSCxY-XL-8x37_xF6SclbSoi6SJRQwhtC-4YwxXkjHqEdFbxtJBHfHqMdYbJrup7RM_QspQMhVEnKn6IzThRXbad26OfleCjeZncH2PjsFmdjGJyZ8boPOYxHb-oVznuIZj3ikpy_xQvk_XEGD3iYC1zUk_FhxlOIS5lNdsFj5zH8WCG6BXzeaHUbxlAnZJe26ujMALmio8npHU77EHOTIS44QipzTs_Rk8nMCV48rOfo64f3X64-Njefrz9dXd40tn4hN9ZMRsoOxt4IZqVSihvWtnzoJkkNtLwHZpm01tiJm6nrJIys5dW_sW87EPwcvTlx1xi-F0hZLy5ZmGfjIZSkGWM96RkRrEpf_yM9hBJ9fZ1mVCkhWC82IDupqpMpRZj0Wm0w8agp0Vtw-hScrsHp--D01vTqAV2GBcY_Lb-TqgJ-EqRa8rcQ_87-D_YXooSoRQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2177442944</pqid></control><display><type>article</type><title>Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results</title><source>SpringerLink Journals - AutoHoldings</source><creator>Filipini, Silvana Martins Rodrigues ; Campagnolo, Cibele Bruno ; Dutra, Danilo Antônio Milbradt ; Maciel, Roberto Marinho ; Danesi, Cristiane Cadermatori ; Kantorski, Karla Zanini</creator><creatorcontrib>Filipini, Silvana Martins Rodrigues ; Campagnolo, Cibele Bruno ; Dutra, Danilo Antônio Milbradt ; Maciel, Roberto Marinho ; Danesi, Cristiane Cadermatori ; Kantorski, Karla Zanini</creatorcontrib><description>The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment,
n
= 5), SRPG (SRP,
n
= 10), aPDTW (SRP+aPDT-MB/water,
n
= 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose,
n
= 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (
p
< 0.05). Percentage of animals that presented mild and severe inflammatory infiltrate was 10% and 40% for SRPG, 20% and 30% for aPDTW, and 50% and 0% for aPDTEt, respectively. BL area (mm
2
) was statistically higher in the LG (0.39 ± 0.15) than NLG (0.05 ± 0.02). aPDTEt showed the lowest value of BL (0.08 ± 0.03), followed by aPDTW (0.21 ± 0.15) and SRPG (0.31 ± 0.18). Statistical differences were verified between aPDTEt and SRPG. In relation to the LG, aPDTEt, aPDTW, and SRPG recovered the equivalent 80%, 46%, and 20% of the BL. aPDTEt showed collagen content statistically higher than SRPG and LG, and presented higher mean values than NLG (
p
> 0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-019-02733-4</identifier><identifier>PMID: 30737587</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Animals ; Antimicrobial agents ; Bone loss ; Carboxymethylcellulose ; Collagen ; Dentistry ; Diabetes ; Diabetes mellitus ; Ethanol ; Gum disease ; Inflammation ; Lasers ; Mandible ; Medicine ; Medicine & Public Health ; Methylene blue ; Optical Devices ; Optics ; Original Article ; Periodontitis ; Photodynamic therapy ; Photonics ; Planing ; Quantum Optics ; Rodents ; Scaling ; Streptozocin</subject><ispartof>Lasers in medical science, 2019-08, Vol.34 (6), p.1253-1260</ispartof><rights>Springer-Verlag London Ltd., part of Springer Nature 2019</rights><rights>Lasers in Medical Science is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-cafa668ed9a42c67773a2553b8f61ae539e2c26ccacf3af886ed253007d958e43</citedby><cites>FETCH-LOGICAL-c375t-cafa668ed9a42c67773a2553b8f61ae539e2c26ccacf3af886ed253007d958e43</cites><orcidid>0000-0002-6219-0255</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-019-02733-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-019-02733-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30737587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filipini, Silvana Martins Rodrigues</creatorcontrib><creatorcontrib>Campagnolo, Cibele Bruno</creatorcontrib><creatorcontrib>Dutra, Danilo Antônio Milbradt</creatorcontrib><creatorcontrib>Maciel, Roberto Marinho</creatorcontrib><creatorcontrib>Danesi, Cristiane Cadermatori</creatorcontrib><creatorcontrib>Kantorski, Karla Zanini</creatorcontrib><title>Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment,
n
= 5), SRPG (SRP,
n
= 10), aPDTW (SRP+aPDT-MB/water,
n
= 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose,
n
= 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (
p
< 0.05). Percentage of animals that presented mild and severe inflammatory infiltrate was 10% and 40% for SRPG, 20% and 30% for aPDTW, and 50% and 0% for aPDTEt, respectively. BL area (mm
2
) was statistically higher in the LG (0.39 ± 0.15) than NLG (0.05 ± 0.02). aPDTEt showed the lowest value of BL (0.08 ± 0.03), followed by aPDTW (0.21 ± 0.15) and SRPG (0.31 ± 0.18). Statistical differences were verified between aPDTEt and SRPG. In relation to the LG, aPDTEt, aPDTW, and SRPG recovered the equivalent 80%, 46%, and 20% of the BL. aPDTEt showed collagen content statistically higher than SRPG and LG, and presented higher mean values than NLG (
p
> 0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.</description><subject>Animals</subject><subject>Antimicrobial agents</subject><subject>Bone loss</subject><subject>Carboxymethylcellulose</subject><subject>Collagen</subject><subject>Dentistry</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Ethanol</subject><subject>Gum disease</subject><subject>Inflammation</subject><subject>Lasers</subject><subject>Mandible</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Methylene blue</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Periodontitis</subject><subject>Photodynamic therapy</subject><subject>Photonics</subject><subject>Planing</subject><subject>Quantum Optics</subject><subject>Rodents</subject><subject>Scaling</subject><subject>Streptozocin</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kctu1DAUhi0EokPhBVggS2zYhPoWO2FXVVCQKrEBiZ3lOCcdjxI7-FIxj8Lb4nQKSCxY-XL-8x37_xF6SclbSoi6SJRQwhtC-4YwxXkjHqEdFbxtJBHfHqMdYbJrup7RM_QspQMhVEnKn6IzThRXbad26OfleCjeZncH2PjsFmdjGJyZ8boPOYxHb-oVznuIZj3ikpy_xQvk_XEGD3iYC1zUk_FhxlOIS5lNdsFj5zH8WCG6BXzeaHUbxlAnZJe26ujMALmio8npHU77EHOTIS44QipzTs_Rk8nMCV48rOfo64f3X64-Njefrz9dXd40tn4hN9ZMRsoOxt4IZqVSihvWtnzoJkkNtLwHZpm01tiJm6nrJIys5dW_sW87EPwcvTlx1xi-F0hZLy5ZmGfjIZSkGWM96RkRrEpf_yM9hBJ9fZ1mVCkhWC82IDupqpMpRZj0Wm0w8agp0Vtw-hScrsHp--D01vTqAV2GBcY_Lb-TqgJ-EqRa8rcQ_87-D_YXooSoRQ</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Filipini, Silvana Martins Rodrigues</creator><creator>Campagnolo, Cibele Bruno</creator><creator>Dutra, 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antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results</title><author>Filipini, Silvana Martins Rodrigues ; Campagnolo, Cibele Bruno ; Dutra, Danilo Antônio Milbradt ; Maciel, Roberto Marinho ; Danesi, Cristiane Cadermatori ; Kantorski, Karla Zanini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-cafa668ed9a42c67773a2553b8f61ae539e2c26ccacf3af886ed253007d958e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antimicrobial agents</topic><topic>Bone loss</topic><topic>Carboxymethylcellulose</topic><topic>Collagen</topic><topic>Dentistry</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Ethanol</topic><topic>Gum disease</topic><topic>Inflammation</topic><topic>Lasers</topic><topic>Mandible</topic><topic>Medicine</topic><topic>Medicine & 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Zanini</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>34</volume><issue>6</issue><spage>1253</spage><epage>1260</epage><pages>1253-1260</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment,
n
= 5), SRPG (SRP,
n
= 10), aPDTW (SRP+aPDT-MB/water,
n
= 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose,
n
= 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (
p
< 0.05). Percentage of animals that presented mild and severe inflammatory infiltrate was 10% and 40% for SRPG, 20% and 30% for aPDTW, and 50% and 0% for aPDTEt, respectively. BL area (mm
2
) was statistically higher in the LG (0.39 ± 0.15) than NLG (0.05 ± 0.02). aPDTEt showed the lowest value of BL (0.08 ± 0.03), followed by aPDTW (0.21 ± 0.15) and SRPG (0.31 ± 0.18). Statistical differences were verified between aPDTEt and SRPG. In relation to the LG, aPDTEt, aPDTW, and SRPG recovered the equivalent 80%, 46%, and 20% of the BL. aPDTEt showed collagen content statistically higher than SRPG and LG, and presented higher mean values than NLG (
p
> 0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.</abstract><cop>London</cop><pub>Springer London</pub><pmid>30737587</pmid><doi>10.1007/s10103-019-02733-4</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-6219-0255</orcidid></addata></record> |
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| ispartof | Lasers in medical science, 2019-08, Vol.34 (6), p.1253-1260 |
| issn | 0268-8921 1435-604X |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Animals Antimicrobial agents Bone loss Carboxymethylcellulose Collagen Dentistry Diabetes Diabetes mellitus Ethanol Gum disease Inflammation Lasers Mandible Medicine Medicine & Public Health Methylene blue Optical Devices Optics Original Article Periodontitis Photodynamic therapy Photonics Planing Quantum Optics Rodents Scaling Streptozocin |
| title | Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results |
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