Direct somatic embryogenesis in a selected tea clone, 'TRI-2025' (Camellia sinensis (L.) O. Kuntze) from nodal explants
An efficient method for the in vitro propagation of a tea (Camellia sinensis (L) O. Kuntze) clone, 'TRI-2025;, from somatic embryos is described. This technique involves two phases; the induction of adventitious buds from nodal cuttings followed by the development of somatic embryos. Single nod...
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| Veröffentlicht in: | Plant cell reports 1998-07, Vol.17 (10), p.804-809 |
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| description | An efficient method for the in vitro propagation of a tea (Camellia sinensis (L) O. Kuntze) clone, 'TRI-2025;, from somatic embryos is described. This technique involves two phases; the induction of adventitious buds from nodal cuttings followed by the development of somatic embryos. Single nodal cuttings were excised from 1-year-old in vitro axenic cultures and inoculated on MS medium with different combinations of IBA/BAP/GA3. Induction of multiple shoots from nodal explants occurred on MS medium with 0.5 mg l-1 BAP, 0.1 mg l-1 IBA and 0.0 mg l-1 GA3 within 6 weeks of incubation. The cultures with multiple shoots were transferred to fresh medium, incubated for 120 days and transferred to MS medium with half-strength macro nutrients, full-strength micronutrients and vitamins and no growth regulator. The direct induction of somatic embryos without callus formation occurred on this medium at 60% frequency within 4 weeks. The production of embryos continued upon transfer of the cultures to fresh medium and a four- to eightfold multiplication rate was obtained during each 6-week culture cycle. The plantlets from these embryos were acclimatised with a 90% success rate. All plants were vigorous and hardy, with well-developed tap-root systems. |
| doi_str_mv | 10.1007/s002990050487 |
| format | Article |
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O. Kuntze) from nodal explants</title><source>SpringerNature Journals</source><creator>Akula, A ; Dodd, W.A</creator><creatorcontrib>Akula, A ; Dodd, W.A</creatorcontrib><description>An efficient method for the in vitro propagation of a tea (Camellia sinensis (L) O. Kuntze) clone, 'TRI-2025;, from somatic embryos is described. This technique involves two phases; the induction of adventitious buds from nodal cuttings followed by the development of somatic embryos. Single nodal cuttings were excised from 1-year-old in vitro axenic cultures and inoculated on MS medium with different combinations of IBA/BAP/GA3. Induction of multiple shoots from nodal explants occurred on MS medium with 0.5 mg l-1 BAP, 0.1 mg l-1 IBA and 0.0 mg l-1 GA3 within 6 weeks of incubation. The cultures with multiple shoots were transferred to fresh medium, incubated for 120 days and transferred to MS medium with half-strength macro nutrients, full-strength micronutrients and vitamins and no growth regulator. The direct induction of somatic embryos without callus formation occurred on this medium at 60% frequency within 4 weeks. The production of embryos continued upon transfer of the cultures to fresh medium and a four- to eightfold multiplication rate was obtained during each 6-week culture cycle. The plantlets from these embryos were acclimatised with a 90% success rate. All plants were vigorous and hardy, with well-developed tap-root systems.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990050487</identifier><identifier>PMID: 30736596</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>benzyladenine ; Biological and medical sciences ; Biotechnology ; Camellia sinensis ; clones ; Cloning ; culture media ; Cuttings ; developmental stages ; dose response ; embryo (plant) ; Embryonic growth stage ; Embryos ; Eukaryotic cell cultures ; explants ; Fundamental and applied biological sciences. Psychology ; gibberellic acid ; Growth regulators ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; indole butyric acid ; methodology ; Methods. Procedures. Technologies ; Micronutrients ; micropropagation ; murashige and skoog medium ; Plant cells and fungal cells ; plant cuttings ; plant morphology ; Shoots ; somatic embryogenesis ; Vitamins</subject><ispartof>Plant cell reports, 1998-07, Vol.17 (10), p.804-809</ispartof><rights>1998 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-b81af26a374cd93c87b1bf4e5714cff6f006d96d2790a0fe2dc7e5b760b8fdfa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2308164$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30736596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akula, A</creatorcontrib><creatorcontrib>Dodd, W.A</creatorcontrib><title>Direct somatic embryogenesis in a selected tea clone, 'TRI-2025' (Camellia sinensis (L.) O. Kuntze) from nodal explants</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>An efficient method for the in vitro propagation of a tea (Camellia sinensis (L) O. Kuntze) clone, 'TRI-2025;, from somatic embryos is described. This technique involves two phases; the induction of adventitious buds from nodal cuttings followed by the development of somatic embryos. Single nodal cuttings were excised from 1-year-old in vitro axenic cultures and inoculated on MS medium with different combinations of IBA/BAP/GA3. Induction of multiple shoots from nodal explants occurred on MS medium with 0.5 mg l-1 BAP, 0.1 mg l-1 IBA and 0.0 mg l-1 GA3 within 6 weeks of incubation. The cultures with multiple shoots were transferred to fresh medium, incubated for 120 days and transferred to MS medium with half-strength macro nutrients, full-strength micronutrients and vitamins and no growth regulator. The direct induction of somatic embryos without callus formation occurred on this medium at 60% frequency within 4 weeks. The production of embryos continued upon transfer of the cultures to fresh medium and a four- to eightfold multiplication rate was obtained during each 6-week culture cycle. The plantlets from these embryos were acclimatised with a 90% success rate. All plants were vigorous and hardy, with well-developed tap-root systems.</description><subject>benzyladenine</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Camellia sinensis</subject><subject>clones</subject><subject>Cloning</subject><subject>culture media</subject><subject>Cuttings</subject><subject>developmental stages</subject><subject>dose response</subject><subject>embryo (plant)</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Eukaryotic cell cultures</subject><subject>explants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gibberellic acid</subject><subject>Growth regulators</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>indole butyric acid</subject><subject>methodology</subject><subject>Methods. Procedures. Technologies</subject><subject>Micronutrients</subject><subject>micropropagation</subject><subject>murashige and skoog medium</subject><subject>Plant cells and fungal cells</subject><subject>plant cuttings</subject><subject>plant morphology</subject><subject>Shoots</subject><subject>somatic embryogenesis</subject><subject>Vitamins</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90c2L1DAUAPAgijuOHr1qEHFnwY4vSZukRxm_FgcWdBe8lTR9Wbq0yZi06PrXm2HGBT1IDjnkl_dJyFMGawag3iQAXtcAFZRa3SMLVgpecBDf7pMFKM4KpVh5Qh6ldAOQH5V8SE4EKCGrWi7Ij3d9RDvRFEYz9Zbi2MbbcI0eU59o76mhCYcssKMTGmqH4PE1Pb38cp6z8OqUrjZmxGHoM-w9-v231XZ9Ri_W9PPsp194Rl0MI_WhMwPFn7vB-Ck9Jg-cGRI-Od5LcvXh_eXmU7G9-Hi-ebstbCn1VLSaGcelEaq0XS2sVi1rXYlVbso6Jx2A7GrZcVWDAYe8swqrVkloteucEUuyOsTdxfB9xjQ1Y59srtd4DHNqOOc16DqPMtNX_6VM5SNFleGLf-BNmKPPbTRal7riZZ7zkhQHZGNIKaJrdrEfTbxtGDT7zTV_bS77Z8egcztid6f_rCqDl0dgkjWDi8bbPt05LkAzuc_7_MCcCY25jplcfeXABPDcZgWV-A2AY6aC</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Akula, A</creator><creator>Dodd, W.A</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>19980701</creationdate><title>Direct somatic embryogenesis in a selected tea clone, 'TRI-2025' (Camellia sinensis (L.) O. Kuntze) from nodal explants</title><author>Akula, A ; Dodd, W.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-b81af26a374cd93c87b1bf4e5714cff6f006d96d2790a0fe2dc7e5b760b8fdfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>benzyladenine</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Camellia sinensis</topic><topic>clones</topic><topic>Cloning</topic><topic>culture media</topic><topic>Cuttings</topic><topic>developmental stages</topic><topic>dose response</topic><topic>embryo (plant)</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Eukaryotic cell cultures</topic><topic>explants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gibberellic acid</topic><topic>Growth regulators</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>indole butyric acid</topic><topic>methodology</topic><topic>Methods. Procedures. Technologies</topic><topic>Micronutrients</topic><topic>micropropagation</topic><topic>murashige and skoog medium</topic><topic>Plant cells and fungal cells</topic><topic>plant cuttings</topic><topic>plant morphology</topic><topic>Shoots</topic><topic>somatic embryogenesis</topic><topic>Vitamins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akula, A</creatorcontrib><creatorcontrib>Dodd, W.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akula, A</au><au>Dodd, W.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct somatic embryogenesis in a selected tea clone, 'TRI-2025' (Camellia sinensis (L.) O. Kuntze) from nodal explants</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>17</volume><issue>10</issue><spage>804</spage><epage>809</epage><pages>804-809</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>An efficient method for the in vitro propagation of a tea (Camellia sinensis (L) O. Kuntze) clone, 'TRI-2025;, from somatic embryos is described. This technique involves two phases; the induction of adventitious buds from nodal cuttings followed by the development of somatic embryos. Single nodal cuttings were excised from 1-year-old in vitro axenic cultures and inoculated on MS medium with different combinations of IBA/BAP/GA3. Induction of multiple shoots from nodal explants occurred on MS medium with 0.5 mg l-1 BAP, 0.1 mg l-1 IBA and 0.0 mg l-1 GA3 within 6 weeks of incubation. The cultures with multiple shoots were transferred to fresh medium, incubated for 120 days and transferred to MS medium with half-strength macro nutrients, full-strength micronutrients and vitamins and no growth regulator. The direct induction of somatic embryos without callus formation occurred on this medium at 60% frequency within 4 weeks. The production of embryos continued upon transfer of the cultures to fresh medium and a four- to eightfold multiplication rate was obtained during each 6-week culture cycle. The plantlets from these embryos were acclimatised with a 90% success rate. All plants were vigorous and hardy, with well-developed tap-root systems.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>30736596</pmid><doi>10.1007/s002990050487</doi><tpages>6</tpages></addata></record> |
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| ispartof | Plant cell reports, 1998-07, Vol.17 (10), p.804-809 |
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| subjects | benzyladenine Biological and medical sciences Biotechnology Camellia sinensis clones Cloning culture media Cuttings developmental stages dose response embryo (plant) Embryonic growth stage Embryos Eukaryotic cell cultures explants Fundamental and applied biological sciences. Psychology gibberellic acid Growth regulators In vitro propagation: entire plant regeneration from tissues and cell cultures indole butyric acid methodology Methods. Procedures. Technologies Micronutrients micropropagation murashige and skoog medium Plant cells and fungal cells plant cuttings plant morphology Shoots somatic embryogenesis Vitamins |
| title | Direct somatic embryogenesis in a selected tea clone, 'TRI-2025' (Camellia sinensis (L.) O. Kuntze) from nodal explants |
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