Use of green fluorescent protein for detection of transformed shoots and homozygous offspring

Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds a...

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Veröffentlicht in:	Plant cell reports 2000-01, Vol.19 (3), p.219-223
Hauptverfasser:	MOLINIER, J, HIMBER, C, HAHNE, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Nicotiana tabacum Offspring Proteins Seedlings Transgenic animals and transgenic plants Transgenic plants
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Beschreibung
Zusammenfassung:	Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds and seedlings of the T1 generation. These results were confirmed by genetic analysis. Optimized conditions for GFP analysis of stable transformants are presented.
ISSN:	0721-7714 1432-203X
DOI:	10.1007/s002990050002