Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens
An efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segments of Astragalus adsurgens. The combinations and concentrations of different growth regulators were shown to be critical factors for both the frequency and the type of callus formation as well as fo...
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| Veröffentlicht in: | Plant cell reports 1998-04, Vol.17 (6-7), p.567-570 |
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| creator | LUO, J.-P JIA, J.-F |
| description | An efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segments of Astragalus adsurgens. The combinations and concentrations of different growth regulators were shown to be critical factors for both the frequency and the type of callus formation as well as for the potential of callus differentiation. Of the four morphologically distinct types of calli that were induced, a friable, yellow callus, i.e. type I, induced on MS medium supplemented with 9.0 µM 2,4-dichlorophenoxyacetic acid and 2.2 µM N
-benzylaminopurine (BA), and then transferred to MS medium containing 0.5 µM α-naphthaleneacetic acid and 8.9 µM BA, exhibited the maximum frequency of shoot regeneration (75%). After regenerated shoots were transferred onto half-strength MS medium without growth regulators, they rooted and complete plants were obtained. Plantlet regeneration from callus cultures required 7-8 weeks. |
| doi_str_mv | 10.1007/s002990050443 |
| format | Article |
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-benzylaminopurine (BA), and then transferred to MS medium containing 0.5 µM α-naphthaleneacetic acid and 8.9 µM BA, exhibited the maximum frequency of shoot regeneration (75%). After regenerated shoots were transferred onto half-strength MS medium without growth regulators, they rooted and complete plants were obtained. Plantlet regeneration from callus cultures required 7-8 weeks.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990050443</identifier><identifier>PMID: 30736637</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Astragalus adsurgens ; Biological and medical sciences ; Biotechnology ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Growth regulators ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; Methods. Procedures. Technologies ; Plant cells and fungal cells</subject><ispartof>Plant cell reports, 1998-04, Vol.17 (6-7), p.567-570</ispartof><rights>1998 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-4c0923d6c497765790b53bd069e54cde4772a680a8c0ea62d5fe822a1c86bebd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2196896$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30736637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LUO, J.-P</creatorcontrib><creatorcontrib>JIA, J.-F</creatorcontrib><title>Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>An efficient procedure was developed for inducing callus and plant regeneration using hypocotyl segments of Astragalus adsurgens. The combinations and concentrations of different growth regulators were shown to be critical factors for both the frequency and the type of callus formation as well as for the potential of callus differentiation. Of the four morphologically distinct types of calli that were induced, a friable, yellow callus, i.e. type I, induced on MS medium supplemented with 9.0 µM 2,4-dichlorophenoxyacetic acid and 2.2 µM N
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The combinations and concentrations of different growth regulators were shown to be critical factors for both the frequency and the type of callus formation as well as for the potential of callus differentiation. Of the four morphologically distinct types of calli that were induced, a friable, yellow callus, i.e. type I, induced on MS medium supplemented with 9.0 µM 2,4-dichlorophenoxyacetic acid and 2.2 µM N
-benzylaminopurine (BA), and then transferred to MS medium containing 0.5 µM α-naphthaleneacetic acid and 8.9 µM BA, exhibited the maximum frequency of shoot regeneration (75%). After regenerated shoots were transferred onto half-strength MS medium without growth regulators, they rooted and complete plants were obtained. Plantlet regeneration from callus cultures required 7-8 weeks.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>30736637</pmid><doi>10.1007/s002990050443</doi><tpages>4</tpages></addata></record> |
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| subjects | Astragalus adsurgens Biological and medical sciences Biotechnology Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Growth regulators In vitro propagation: entire plant regeneration from tissues and cell cultures Methods. Procedures. Technologies Plant cells and fungal cells |
| title | Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens |
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