Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L
The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mM solution of the nuclease...
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| Veröffentlicht in: | Plant cell reports 1998-06, Vol.17 (9), p.737-741 |
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| creator | Barandiaran, X Di Pietro, A Martin, J |
| description | The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mM solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results. |
| doi_str_mv | 10.1007/s002990050475 |
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These tissues showed a high endogenous nuclease activity preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mM solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s002990050475</identifier><identifier>PMID: 30736535</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Allium sativum ; aurintricarboxylic acid ; Biological and medical sciences ; Biotechnology ; bulbs ; callus ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; gene expression ; gene transfer ; Genetic engineering ; Genetic technics ; genetic transformation ; Genetics ; inhibition ; leaves ; localization ; Methods. Procedures. Technologies ; Modification of gene expression level ; nucleases ; plasmids ; reporter genes ; Tissues ; transgenic plants ; tricarboxylic acids</subject><ispartof>Plant cell reports, 1998-06, Vol.17 (9), p.737-741</ispartof><rights>1998 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-4cd23ee99e1f9582d3c4b382052d46c0b0e86b38ab77ea9f5510d248bbd293713</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2306073$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30736535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barandiaran, X</creatorcontrib><creatorcontrib>Di Pietro, A</creatorcontrib><creatorcontrib>Martin, J</creatorcontrib><title>Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mM solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results.</description><subject>Allium sativum</subject><subject>aurintricarboxylic acid</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>bulbs</subject><subject>callus</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>gene transfer</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Genetics</subject><subject>inhibition</subject><subject>leaves</subject><subject>localization</subject><subject>Methods. Procedures. Technologies</subject><subject>Modification of gene expression level</subject><subject>nucleases</subject><subject>plasmids</subject><subject>reporter genes</subject><subject>Tissues</subject><subject>transgenic plants</subject><subject>tricarboxylic acids</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp90U2LFDEQBuAgijuuHr1qEBEvrZVK0ukcx8UvGPCgC96adFK9ZOnpjEn3ov_eLDMu6MFTQepJUcXL2FMBbwSAeVsA0FoADcroe2wjlMQGQX6_zzZgUDTGCHXGHpVyDVCbpn3IziQY2WqpN2x4F9MUyxI9X7Kby0iZuzlw-nnIVEpMM08jd3yNYcszHVJeqriimXiceYhj_UDzwpdYykrlFm-nKa57XtwSb2rdPWYPRjcVenKq5-zyw_tvF5-a3ZePny-2u8YrwKVRPqAkspbEaHWHQXo1yA5BY1CthwGoa-uDG4whZ0etBQRU3TAEtNIIec5eH-cecvpRd1n6fSyepsnNlNbSI6IFY42Wlb76LxVGtBWqCl_8A6_Tmud6Rt91qtPYWqyoOSKfUymZxv6Q497lX72A_jak_q-Qqn92GroOewp3-k8qFbw8AVe8m8YajI_lzqGEttLKnh_Z6FLvrnIll18RhATsrJAo5G_PAqDf</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Barandiaran, X</creator><creator>Di Pietro, A</creator><creator>Martin, J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L</title><author>Barandiaran, X ; Di Pietro, A ; Martin, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-4cd23ee99e1f9582d3c4b382052d46c0b0e86b38ab77ea9f5510d248bbd293713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Allium sativum</topic><topic>aurintricarboxylic acid</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>bulbs</topic><topic>callus</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>gene transfer</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Genetics</topic><topic>inhibition</topic><topic>leaves</topic><topic>localization</topic><topic>Methods. Procedures. Technologies</topic><topic>Modification of gene expression level</topic><topic>nucleases</topic><topic>plasmids</topic><topic>reporter genes</topic><topic>Tissues</topic><topic>transgenic plants</topic><topic>tricarboxylic acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barandiaran, X</creatorcontrib><creatorcontrib>Di Pietro, A</creatorcontrib><creatorcontrib>Martin, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barandiaran, X</au><au>Di Pietro, A</au><au>Martin, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>17</volume><issue>9</issue><spage>737</spage><epage>741</epage><pages>737-741</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mM solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>30736535</pmid><doi>10.1007/s002990050475</doi><tpages>5</tpages></addata></record> |
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| subjects | Allium sativum aurintricarboxylic acid Biological and medical sciences Biotechnology bulbs callus enzyme activity Fundamental and applied biological sciences. Psychology gene expression gene transfer Genetic engineering Genetic technics genetic transformation Genetics inhibition leaves localization Methods. Procedures. Technologies Modification of gene expression level nucleases plasmids reporter genes Tissues transgenic plants tricarboxylic acids |
| title | Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L |
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