Engineering of multiple modular pathways for high-yield production of 5-aminolevulinic acid in Escherichia coli

•Balancing the expression of hemA and hemL by mutating RBS improved ALA synthesis.•ALA catabolism was weakened by substituting the promoter of hemB with fliCp.•Strengthening the native synthesis pathway of PLP improved GST-AT activity.•ALA production increased to 5.25 g/L with a pH two-stage strateg...

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Veröffentlicht in:	Bioresource technology 2019-02, Vol.274, p.353-360
Hauptverfasser:	Zhang, Junli, Weng, Huanjiao, Zhou, Zhengxiong, Du, Guocheng, Kang, Zhen
Format:	Artikel
Sprache:	eng
Schlagworte:	5-aminolevulinic acid ALA dehydratase Aminolevulinic Acid biosynthesis Biosynthetic Pathways catabolism Cloning, Molecular Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism fermenters Intramolecular Transferases - metabolism Levulinic Acids - metabolism medicine Metabolic Engineering mutation plasmids pyridoxal Pyridoxal 5′-phosphate technology
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