Probing membrane enhanced protein-protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase
Investigating the interplay in a minimal redox complex of cytochrome-P450 and its reductase is crucial for understanding cytochrome-P450's enzymatic activity. Probing the hotspots of dynamic structural interactions using NMR revealed the engagement of loop residues from P450-reductase to be res...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2019-05, Vol.55 (41), p.5777-578 |
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| container_issue | 41 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Mahajan, Mukesh Ravula, Thirupathi Prade, Elke Anantharamaiah, G. M Ramamoorthy, Ayyalusamy |
| description | Investigating the interplay in a minimal redox complex of cytochrome-P450 and its reductase is crucial for understanding cytochrome-P450's enzymatic activity. Probing the hotspots of dynamic structural interactions using NMR revealed the engagement of loop residues from P450-reductase to be responsible for the enhanced affinity of CYP450 towards its obligate redox partner.
Investigating the interplay in a minimal redox complex of cytochrome-P450 and its reductase is crucial for understanding cytochrome-P450's enzymatic activity. |
| doi_str_mv | 10.1039/c9cc01630a |
| format | Article |
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| ispartof | Chemical communications (Cambridge, England), 2019-05, Vol.55 (41), p.5777-578 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Animals Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - metabolism Humans Models, Molecular NADPH-Ferrihemoprotein Reductase - chemistry NADPH-Ferrihemoprotein Reductase - metabolism Nuclear Magnetic Resonance, Biomolecular - methods Oxidation-Reduction Protein Interaction Mapping - methods Protein Interaction Maps Rabbits |
| title | Probing membrane enhanced protein-protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase |
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